The chromosome complement of the Rhesus monkey (Macaca mulatta) determined in kidney cells cultivated in vitro

Chromosoma ◽  
1957 ◽  
Vol 9 (1) ◽  
pp. 163-175 ◽  
Author(s):  
K. H. Rothfels ◽  
L. Siminovitch
Keyword(s):  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Chromosome Complement ◽  
Kidney Cells

65 BLASTOCYSTS DERIVED FROM ADULT FIBROBLASTS OF RHESUS MONKEY (MACACA MULATTA) USING INTERSPECIES SOMATIC CELL NUCLEAR TRANSFER

2010 ◽  
Vol 22 (1) ◽  
pp. 191
Author(s):  
D. K. Kwon ◽  
J. T. Kang ◽  
S. J. Park ◽  
M. N. L. Gomez ◽  
S. J. Kim ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Somatic Cell ◽  
Macaca Mulatta ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Developmental Rate ◽  
Cell Stage ◽  
Nuclear Number

Interspecies somatic cell nuclear transfer (iSCNT) has alternatively chosen in primate SCNT because of the difficulty in collecting enough oocytes for research. The purpose of this experiment is to produce iSCNT-derived blastocysts using enucleated cow (Bos taurus) metaphase II oocytes and adult rhesus monkey (Macaca mulatta) fibroblasts. Ear skin tissueofrhesus monkey (male, 6 years old) was collected by biopsy and fibroblasts were isolated. Immature COCs from cow ovaries were collected and matured in vitro in TCM-199. Squish enucleation was done in the presence of bisbenzimide and cytochalasin B. After enucleation, a single rhesus monkey somatic cell was injected into the perivitelline space of an enucleated oocyte through the slit in the zona pellucida made during enucleation. Subsequently, the rhesus monkey somatic cell and cow oocyte membranes were electrically fused. The nonactivated interspecies cloned couplets were cultured for 2 h to allow reprogramming to occur. Then, couplets were activated using a 2-step protocol consisting of treatment with 5 μM ionomycin for 4 to 5 min and subsequently with 2mM 6-DMAP for 4 h. Activated iSCNT embryos were cultured for 10 days inmodified SOF with various conditions (at 37 to39°C, 5 to 5.5% CO2 and 5 to 20% O2) to examine the effects ofIVC conditions. As a results, most embryos were arrested at the 8- to 16-cell stage and only 3 blastocysts were derived from rhesus monkey iSCNT. The blastocyst developmental rate was 0.26% generated from the total IVC activated interspecies embryos (n = 1153). Among the 3 blastocysts, 2 of them were used for counting nuclear number using bisbenzimide staining. The nuclear number of the 2 iSCNT-derived blastocysts was 51 and 24, respectively. The other iSCNT-derived blastocyst was used for analyzing mitochondrial (mt)DNAto confirm that it contained both cow and rhesus monkey mtDNA. As a result, mtDNA from both rhesus monkey and cow were detected inPCR analysis. The band intensity was more dominant for cow mtDNA than for rhesus monkey mtDNA. Although the blastocyst developmental rate is extremely low, it is confirmed that two phylogenetically distant species including primate could develop in vitro until the blastocyst stage by iSCNT. The in vitro developmental system of this rhesus monkey iSCNT-derived blastocysts provides a platform for further improvement of developmental rate and quality of rhesus monkey iSCNT-derived blastocysts. It also provides an opportunity to establish rhesus monkey iSCNT-derived embryonic stem cell lines for study of rhesus monkey nucleus and cow mitochondria interaction mechanisms during early developmental stages. This study was financially supported by the Korean MEST, through the BK21 program for Veterinary Science, and SNU foundation (Benefactor; RNL Bio).

Blastocysts derived from adult fibroblasts of a rhesus monkey (Macaca mulatta) using interspecies somatic cell nuclear transfer

Zygote ◽  
2011 ◽  
Vol 19 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Dae Kee Kwon ◽  
Jung Taek Kang ◽  
Sol Ji Park ◽  
Ma Ninia Limas Gomez ◽  
Su Jin Kim ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Somatic Cell ◽  
Macaca Mulatta ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Development Rate ◽  
Attractive Alternative ◽  
Cell Stage

SummaryIn non-human primates, it is difficult to collect sufficient numbers of oocytes for producing identical embryos by somatic cell nuclear transfer (SCNT). Because of this factor, inter-species SCNT (iSCNT) using heterospecific oocytes is an attractive alternative approach. The objective of this study was to produce iSCNT-derived blastocysts using enucleated cow (Bos taurus) metaphase II oocytes and adult rhesus monkey (Macaca mulatta) fibroblasts. Ear skin tissue from a 6-year-old male rhesus monkey was collected by biopsy and fibroblasts were isolated. Immature cumulus–oocyte complexes from cow ovaries were collected and matured in vitro in Medium 199. The enucleated oocytes were reconstructed with rhesus monkey fibroblasts and iSCNT embryos were cultured in modified synthetic oviduct fluid in an atmosphere of 5–5.5% CO2 under various conditions (37–39 °C and 5–20% O2) to examine the effects of in vitro culture conditions. Most embryos were arrested at the 8- or 16-cell stage and only three blastocysts were derived in this way using iSCNT from a total of 1153 cultured activated embryos (0.26% production rate). Two of the three blastocysts were used for counting nuclear numbers using bisbenzimide staining, which were 51 and 24. The other iSCNT-derived blastocyst was used to analyse mitochondrial DNA (mtDNA) by PCR, and both rhesus monkey and cow mtDNA were detected. Although the development rate was extremely low, this study established that iSCNT using two phylogenetically distant species, including a primate, could produce blastocysts. With improvements in the development rate, it may be possible to produce rhesus monkey iSCNT-derived embryonic stem cell lines for studies on primate nucleus and cow mitochondria interaction mechanisms.

PRODUCTION OF THROMBOXANE B2 BY INTRA-UTERINE TISSUES FROM LATE PREGNANT RHESUS MONKEYS (MACACA MULATTA) IN VITRO

1978 ◽  
Vol 79 (1) ◽  
pp. 103-106 ◽  
Author(s):  
M. D. MITCHELL ◽  
B. R. HICKS ◽  
G. D. THORBURN ◽  
J. S. ROBINSON
Keyword(s):  
Caesarean Section ◽  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Late Pregnancy ◽  
Significant Trend ◽  
Thromboxane B2 ◽  
Decidua Basalis

The rates of production of thromboxane B2 in vitro by intra-uterine tissues obtained from late pregnant monkeys by Caesarean section have been determined. The general quantitative order of rates of production was decidua basalis = decidua parietalis > placenta > chorion > amnion = myometrium. Myometrial production of thromboxane B2 was greater at term than during late pregnancy; no other tissue showed a significant trend with advancing gestation. These data demonstrate that the production of thromboxane B2 by intra-uterine tissues from late pregnant monkeys is both qualitatively and quantitatively different from the production of prostaglandins described previously. It is suggested that prostaglandins rather than thromboxanes are more intimately involved in the onset of labour in the rhesus monkey.

In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta)

2011 ◽  
Vol 47 (5-6) ◽  
pp. 383-390 ◽  
Author(s):  
Shaohui Ji ◽  
Xihong Wang ◽  
Jianhong Shu ◽  
Aijing Sun ◽  
Wei Si ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Progenitor Cells ◽  
Macaca Mulatta ◽  
Epithelial Progenitor Cells

SPECIFIC CHANGE IN THE DIRECTION OF PROSTAGLANDIN SYNTHESIS BY INTRA-UTERINE TISSUES OF THE RHESUS MONKEY (MACACA MULATTA) DURING LATE PREGNANCY

1978 ◽  
Vol 78 (3) ◽  
pp. 343-350 ◽  
Author(s):  
M. D. MITCHELL ◽  
L. CLOVER ◽  
G. D. THORBURN ◽  
J. S. ROBINSON
Keyword(s):  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Late Pregnancy ◽  
Prostaglandin Synthesis ◽  
Unit Weight ◽  
Prostaglandin E ◽  
Specific Change ◽  
Decidua Basalis ◽  
Prostaglandin F

The rates of production of prostaglandin E (PGE), prostaglandin F (PGF) and 13,14-dihydro-15-oxo-prostaglandin F (PGFM) by intra-uterine tissues from pregnant monkeys in vitro have been determined using a method of tissue superfusion. The amnion, chorion, placenta, decidua basalis, decidua parietalis and myometrium were obtained at Caesarean section during late pregnancy. Production of PGE by all tissues was significantly lower at term than during late pregnancy, whereas production of PGF by the amnion, chorion, decidua parietalis and myometrium was significantly greater. All tissues produced significantly more PGE than PGF and also, excepting the decidua basalis and decidua parietalis, more PGFM than PGF. Close to parturition the amnion was quantitatively (per unit weight) the major source of prostaglandins. It is suggested that a specific change in the direction of prostaglandin synthesis by intra-uterine tissues occurs near parturition in the rhesus monkey.

scholarly journals 95FIRST REPORT ON CLEAVAGE DEVELOPMENT FOLLOWING CRYOPRESERVATION OF ADULT AND PREPUBERTAL RHESUS MONKEY (MACACA MULATTA) OOCYTES

2004 ◽  
Vol 16 (2) ◽  
pp. 169 ◽  
Author(s):  
A. Dinnyes ◽  
S. Wei ◽  
Y. Li ◽  
P. Zheng ◽  
W. Ji
Keyword(s):  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Cleavage Stage ◽  
Chi Square ◽  
Cell Stage ◽  
Chilling Sensitivity ◽  
Stage Development ◽  
Vitrification Solution ◽  
Human Primate

Cryopreservation of non-human primate oocytes would allow a more efficient management of biological resources for medical research and endangered species preservation. Despite previous attempts, no cleavage-stage development has been reported following cryopreservation of rhesus monkey (Macaca mulatta) or other non-human primate oocytes. The extreme chilling sensitivity of rhesus monkey oocytes (Songsasen N et al., 2002 Fertil. Steril. 77, 818–825) might be overcomed by vitrification methods. Our aim was to test the Solid Surface Vitrification (SSV, Dinnyes A et al., 2000 Biol. Reprod. 63, 513–518) method on metaphase II (MII)-stage rhesus monkey oocytes and to achieve successful fertilization of warmed oocytes. Oocytes were obtained from hormonally stimulated adult and unstimulated prepubertal females and matured in vitro for 36 to 48h as described by Zhang P et al. (2001 Biol. Reprod. 64, 1417–1421). The vitrification solution contained 35% ethylene glycol (EG), 5% polyvinyl pyrrolidone, and 0.4M trehalose in Tyrode-lactate (TL)-HEPES medium with 3mgmL−1 BSA added. Oocytes at MII-stage were equilibrated in 4% EG in TL-HEPES at 37°C for 10 to 12min and then exposed to the vitrification solution at 37°C for about 20s. Oocytes in 2-μL droplets of vitrification solution were dropped onto a metal surface at about −180°C where they were vitrified instantaneously. Warming was performed by moving the vitrified droplets into 0.3M trehalose at 37°C. Recovered oocytes were exposed to 0.15M and then 0.075M trehalose for 2min each and then rinsed three times in TL-HEPES. Warmed oocytes were fertilized in vitro and then cultured for 96h in 50-μL drops of mCMRL-1066 containing 20% FCS at 37°C in a humidified atmosphere of 5% CO2, 5% O2, and 90% N2, as described in details by Zhang (see above). A total of 21 MII oocytes were collected from 2 adult and 55 MII oocytes from 14 prepubertal animals. No difference has been found between the rate of adult-origin (15/19; 79%) v. prepubertal-origin (37/52; 71%) oocytes surviving the vitrification process without lysis (P>0.05, chi-square analyses). Following subsequent IVF, 1/15 (7%) adult-origin and 4/23 (17%) prepubertal-origin oocytes cleaved further, which was lower than that of the corresponding controls (1/2 (50%) and 1/3 (33%), respectively). The furthest development observed following cryopreservation was 16-cell stage, verified by counting of stained nuclei. This is the first report on cleavage-stage development of cryopreserved rhesus monkey oocytes, demonstrating the feasibility of vitrification and the potential for gene banking of non-human primate oocytes, even from prepubertal animals. Further experiments are needed to achieve higher rates of cryosurvival and progeny development. This research was supported by a Chinese-Hungarian Bilateral Technological and Scientific Collaboration Project (No. CHN14/02).

scholarly journals Post-translational modifications in glycosylation status during epididymal passage and significance in fertility of a 33 kDa glycoprotein (MEF3) of rhesus monkey (Macaca mulatta)

Reproduction ◽  
2008 ◽  
Vol 135 (6) ◽  
pp. 761-770 ◽  
Author(s):  
Abhishek Chandra ◽  
Kokattam Rama Srinivasan ◽  
Farrukh Jamal ◽  
Puroshottam Kumar Mehrotra ◽  
Ram Lakhan Singh ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Human Spermatozoa ◽  
Sperm Function ◽  
Dependent Manner ◽  
Western Blots ◽  
Post Translational Modifications ◽  
Epididymal Fluid

The present study reports data on post-translational modifications in the glycosylation status during epididymal passage and significance in fertility of a 33 kDa glycoprotein of rhesus monkey (Macaca mulatta), designated as MEF3 (monkey epididymal fluid protein 3). MEF3 exhibited strong affinity for N-linked α-d-mannose groups and O-linkedN-Ac-galactosamine linkages in epididymal fluids and exhibited moderate affinity forN-Ac-glucosaminylated (wheat germ agglutinin), fucosylated (Tetragonolotus purpurea), andN-Ac-galactosamine (peanut agglutinin) residues on more mature corpus and caudal spermatozoa in a maturation-dependent manner on Western blots probed with specific biotinylated lectins. Polyclonal antiserum raised against affinity-purified MEF3 from caudal epididymal fluid (CEF) cross-reacted specifically with CEF and caudal sperm membrane of macaque and with Triton X-100 extract of ejaculated human spermatozoa, suggesting the existence of antigenically related components in both species. The tangled agglutination caused by anti-33 kDa serum of human spermatozoa, along with localization of MEF3 on entire sperm surface of epididymal and testicular sperm of monkey and human spermatozoa, suggest the significance of MEF3 in sperm function. The 100% inhibition of fertility of immunized female rabbits with this proteinin vivoand inhibition of human sperm penetration in zona-free hamster eggsin vitrosuggests the functional significance of MEF3 in fertility. Together, these results clearly indicate that MEF3 has potential significance as a target for antibodies that inhibit sperm function and fertility.

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