PRODUCTION OF THROMBOXANE B2 BY INTRA-UTERINE TISSUES FROM LATE PREGNANT RHESUS MONKEYS (MACACA MULATTA) IN VITRO

1978 ◽  
Vol 79 (1) ◽  
pp. 103-106 ◽  
Author(s):  
M. D. MITCHELL ◽  
B. R. HICKS ◽  
G. D. THORBURN ◽  
J. S. ROBINSON
Keyword(s):  
Caesarean Section ◽  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Late Pregnancy ◽  
Significant Trend ◽  
Thromboxane B2 ◽  
Decidua Basalis

The rates of production of thromboxane B2 in vitro by intra-uterine tissues obtained from late pregnant monkeys by Caesarean section have been determined. The general quantitative order of rates of production was decidua basalis = decidua parietalis > placenta > chorion > amnion = myometrium. Myometrial production of thromboxane B2 was greater at term than during late pregnancy; no other tissue showed a significant trend with advancing gestation. These data demonstrate that the production of thromboxane B2 by intra-uterine tissues from late pregnant monkeys is both qualitatively and quantitatively different from the production of prostaglandins described previously. It is suggested that prostaglandins rather than thromboxanes are more intimately involved in the onset of labour in the rhesus monkey.

INTRA-UTERINE TISSUES FROM LATE-PREGNANT RHESUS MONKEYS (MACACA MULATTA) PRODUCE 6-OXO-PROSTAGLANDIN F1α IN VITRO

1979 ◽  
Vol 81 (3) ◽  
pp. 339-343 ◽  
Author(s):  
M. D. MITCHELL ◽  
B. R. HICKS ◽  
G. D. THORBURN ◽  
J. S. ROBINSON
Keyword(s):  
Caesarean Section ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Late Pregnancy ◽  
Unit Weight ◽  
Decidua Basalis ◽  
Prostaglandin F ◽  
Near Term

The rates of production of 6-oxo-prostaglandin F1α (6-oxo-PGF1α) in vitro by intra-uterine tissues taken from late-pregnant monkeys at Caesarean section have been determined. For tissues obtained between days 140 and 149 of pregnancy (late pregnancy) the general quantitative order of rates of production (per unit weight) was decidua basalis> placenta > decidua parietalis>amnion>chorion = myometrium. When tissues were taken between days 160 and 168 of pregnancy (near term) this order was placenta > decidua parietalis = amnion> myometrium = decidua basalis > chorion. There was a significant reduction near term in the rate of production of 6-oxo-PGF1α by decidua basalis; all other tissues exhibited similar rates of production at the two gestational periods investigated.

SPECIFIC CHANGE IN THE DIRECTION OF PROSTAGLANDIN SYNTHESIS BY INTRA-UTERINE TISSUES OF THE RHESUS MONKEY (MACACA MULATTA) DURING LATE PREGNANCY

1978 ◽  
Vol 78 (3) ◽  
pp. 343-350 ◽  
Author(s):  
M. D. MITCHELL ◽  
L. CLOVER ◽  
G. D. THORBURN ◽  
J. S. ROBINSON
Keyword(s):  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Late Pregnancy ◽  
Prostaglandin Synthesis ◽  
Unit Weight ◽  
Prostaglandin E ◽  
Specific Change ◽  
Decidua Basalis ◽  
Prostaglandin F

The rates of production of prostaglandin E (PGE), prostaglandin F (PGF) and 13,14-dihydro-15-oxo-prostaglandin F (PGFM) by intra-uterine tissues from pregnant monkeys in vitro have been determined using a method of tissue superfusion. The amnion, chorion, placenta, decidua basalis, decidua parietalis and myometrium were obtained at Caesarean section during late pregnancy. Production of PGE by all tissues was significantly lower at term than during late pregnancy, whereas production of PGF by the amnion, chorion, decidua parietalis and myometrium was significantly greater. All tissues produced significantly more PGE than PGF and also, excepting the decidua basalis and decidua parietalis, more PGFM than PGF. Close to parturition the amnion was quantitatively (per unit weight) the major source of prostaglandins. It is suggested that a specific change in the direction of prostaglandin synthesis by intra-uterine tissues occurs near parturition in the rhesus monkey.

PROSTAGLANDIN E, THROMBOXANE B2 AND 6-OXO-PROSTAGLANDIN F1α IN AMNIOTIC FLUID AND MATERNAL PLASMA OF RHESUS MONKEYS (MACACA MULATTA) DURING THE LATTER THIRD OF GESTATION

1979 ◽  
Vol 81 (3) ◽  
pp. 345-349 ◽  
Author(s):  
J. S. ROBINSON ◽  
R. NATALE ◽  
L. CLOVER ◽  
M. D. MITCHELL
Keyword(s):  
Rhesus Monkey ◽  
Amniotic Fluid ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Maternal Plasma ◽  
Thromboxane B2 ◽  
Prostaglandin E ◽  
Peripheral Plasma ◽  
Prostaglandin F ◽  
Serial Samples

The concentrations of prostaglandin E (PGE), thromboxane B2 (TXB2) and 6-oxo-prostaglandin F1α (6-oxo-PGF1α) were measured by radioimmunoassay in serial samples of amniotic fluid and maternal peripheral plasma in the latter third of pregnancy in rhesus monkeys (Macaca mulatta). The samples were collected under ketamine-induced anaesthesia. The concentration of PGE was undetectable in amniotic fluid until a few days before delivery when a large increase was observed in three of the five animals. There were small increases of TXB2 and 6-oxo-PGF1α in amniotic fluid before delivery. In maternal plasma the concentrations of PGE, TXB2 and 6-oxo-PGF1α were generally higher and more variable than in amniotic fluid and did not increase with advancing gestation. It is suggested that increased production of primary prostaglandins occurs before, and is involved in, the onset of parturition in the rhesus monkey.

STEROIDS IN THE AMNIOTIC FLUID OF THE RHESUS MONKEY (MACACA MULATTA)

1977 ◽  
Vol 73 (2) ◽  
pp. 355-363 ◽  
Author(s):  
J. R. G. CHALLIS ◽  
PHILLIDA HARTLEY ◽  
P. JOHNSON ◽  
J. E. PATRICK ◽  
J. S. ROBINSON ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Amniotic Fluid ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Plasma Cortisol ◽  
Late Pregnancy ◽  
Maternal Plasma ◽  
Steroid Secretion ◽  
Adrenal Steroid ◽  
Oestrone Sulphate

SUMMARY Representative C21, C19 and C18 steroids have been measured in the amniotic fluid and maternal femoral plasma of rhesus monkeys during the last 50 days of pregnancy. The concentrations of cortisol, progesterone, androstenedione, oestrone and oestrone sulphate all increased in amniotic fluid before spontaneous parturition. In contrast, amniotic fluid oestradiol levels remained low and unchanged, and were always less than the concentrations in the maternal plasma. The changes in progesterone, cortisol and oestrone concentrations in amniotic fluid occurred independently of significant changes in the concentration of these steroids in the maternal plasma. Betamethasone administered to monkeys after day 150 of pregnancy suppressed amniotic fluid and maternal plasma cortisol. This treatment markedly suppressed the concentration of progesterone in amniotic fluid, but did not alter its concentration in maternal plasma. It is concluded that during late pregnancy in the rhesus monkey there is an increase in the concentration of some steroids in amniotic fluid independent of alterations in their concentrations in maternal plasma. These changes could reflect an increase in foetal adrenal steroid secretion.

65 BLASTOCYSTS DERIVED FROM ADULT FIBROBLASTS OF RHESUS MONKEY (MACACA MULATTA) USING INTERSPECIES SOMATIC CELL NUCLEAR TRANSFER

2010 ◽  
Vol 22 (1) ◽  
pp. 191
Author(s):  
D. K. Kwon ◽  
J. T. Kang ◽  
S. J. Park ◽  
M. N. L. Gomez ◽  
S. J. Kim ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Somatic Cell ◽  
Macaca Mulatta ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Developmental Rate ◽  
Cell Stage ◽  
Nuclear Number

Interspecies somatic cell nuclear transfer (iSCNT) has alternatively chosen in primate SCNT because of the difficulty in collecting enough oocytes for research. The purpose of this experiment is to produce iSCNT-derived blastocysts using enucleated cow (Bos taurus) metaphase II oocytes and adult rhesus monkey (Macaca mulatta) fibroblasts. Ear skin tissueofrhesus monkey (male, 6 years old) was collected by biopsy and fibroblasts were isolated. Immature COCs from cow ovaries were collected and matured in vitro in TCM-199. Squish enucleation was done in the presence of bisbenzimide and cytochalasin B. After enucleation, a single rhesus monkey somatic cell was injected into the perivitelline space of an enucleated oocyte through the slit in the zona pellucida made during enucleation. Subsequently, the rhesus monkey somatic cell and cow oocyte membranes were electrically fused. The nonactivated interspecies cloned couplets were cultured for 2 h to allow reprogramming to occur. Then, couplets were activated using a 2-step protocol consisting of treatment with 5 μM ionomycin for 4 to 5 min and subsequently with 2mM 6-DMAP for 4 h. Activated iSCNT embryos were cultured for 10 days inmodified SOF with various conditions (at 37 to39°C, 5 to 5.5% CO2 and 5 to 20% O2) to examine the effects ofIVC conditions. As a results, most embryos were arrested at the 8- to 16-cell stage and only 3 blastocysts were derived from rhesus monkey iSCNT. The blastocyst developmental rate was 0.26% generated from the total IVC activated interspecies embryos (n = 1153). Among the 3 blastocysts, 2 of them were used for counting nuclear number using bisbenzimide staining. The nuclear number of the 2 iSCNT-derived blastocysts was 51 and 24, respectively. The other iSCNT-derived blastocyst was used for analyzing mitochondrial (mt)DNAto confirm that it contained both cow and rhesus monkey mtDNA. As a result, mtDNA from both rhesus monkey and cow were detected inPCR analysis. The band intensity was more dominant for cow mtDNA than for rhesus monkey mtDNA. Although the blastocyst developmental rate is extremely low, it is confirmed that two phylogenetically distant species including primate could develop in vitro until the blastocyst stage by iSCNT. The in vitro developmental system of this rhesus monkey iSCNT-derived blastocysts provides a platform for further improvement of developmental rate and quality of rhesus monkey iSCNT-derived blastocysts. It also provides an opportunity to establish rhesus monkey iSCNT-derived embryonic stem cell lines for study of rhesus monkey nucleus and cow mitochondria interaction mechanisms during early developmental stages. This study was financially supported by the Korean MEST, through the BK21 program for Veterinary Science, and SNU foundation (Benefactor; RNL Bio).

An enzyme-linked immunosorbent assay to study human relaxin in human pregnancy and in pregnant rhesus monkeys

1989 ◽  
Vol 120 (3) ◽  
pp. 449-457 ◽  
Author(s):  
C. Lucas ◽  
L. N. Bald ◽  
M. C. Martin ◽  
R. B. Jaffe ◽  
D. W. Drolet ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Pregnant Women ◽  
Human Insulin ◽  
Rhesus Monkeys ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Corpora Lutea ◽  
Synthetic Analogue ◽  
Human Pregnancy

ABSTRACT A sensitive and specific double-antibody enzyme-linked immunoassay, using a synthetic analogue of human relaxin for standard and immunogen, was developed for the measurement of human relaxin (hRLX) in serum and plasma. No cross-reactivity was observed for human insulin, human insulin-like growth factor-I, hGH, human chorionic gonadotropin, hFSH, hLH or human prolactin. The assay was used to monitor RLX concentrations in samples from men, non-pregnant and pregnant women, and in pregnant rhesus monkeys infused with hRLX. RLX was not detected in serum from men nor from non-pregnant women, while a concentration of 600 ng/l was measured in pooled sera from two pregnant women (pregnancies achieved by in-vitro fertilization). Immunoreactive RLX (1·1 μg/g) was found in human corpora lutea taken from ectopic pregnancies at 7 weeks. In an experiment with a pregnant rhesus monkey infused with human RLX analogue, less than 1·5% of the maternal concentration was measured in the fetal circulation. Even though preliminary, these data suggest a low level of transfer of human analogue relaxin across the placenta in a rhesus monkey. Further studies of the physiology of RLX in human pregnancy will be facilitated by the availability of this immunoassay. Journal of Endocrinology (1989) 120, 449–457

scholarly journals Selection for Urease Activity duringHelicobacter pylori Infection of Rhesus Macaques (Macaca mulatta)

2001 ◽  
Vol 69 (5) ◽  
pp. 3519-3522 ◽  
Author(s):  
Lori M. Hansen ◽  
Jay V. Solnick
Keyword(s):  
Helicobacter Pylori ◽  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Urease Activity ◽  
Rhesus Macaques ◽  
Selection For ◽  
H Pylori ◽  
Selection Of

ABSTRACT Helicobacter pylori strain J166 recovered from experimentally inoculated rhesus monkeys had up to a 250-fold-increased urease activity over that before inoculation. This was found to result from the selection of urease positive J166 clones from a heterogenous inoculum, which was predominantly urease negative due to a 1-bp insertion in the ureA gene. These results confirm the importance of urease for H. pylori colonization. Strain J166 is particularly well adapted to the rhesus monkey, since it colonized preferentially despite the fact that less than 0.1% of the inoculum was urease positive.

THE NUMBER OF OOCYTES IN THE MATURE RHESUS MONKEY (MACACA MULATTA)

1951 ◽  
Vol 7 (2) ◽  
pp. 194-202 ◽  
Author(s):  
S. H. GREEN ◽  
S. ZUCKERMAN
Keyword(s):  
Statistical Analysis ◽  
Menstrual Cycle ◽  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Significant Difference ◽  
Average Total Number ◽  
Sexually Mature

Estimates have been made of the total number of oocytes in ovaries removed on different days of the menstrual cycle from twelve sexually mature rhesus monkeys. They have been analysed on two assumptions: (a) that no relation exists over the range of age studied between age and the number of oocytes present; and (b) that such a relation exists. The data were grouped in the following three temporal phases of the menstrual cycle: days 1–9, days 10–18, days 19–27. Statistical analysis failed to reveal any significant difference between the average total number of oocytes present at the beginning, the middle, or the end of the menstrual cycle.

Sign in / Sign up

Export Citation Format

Share Document