scholarly journals Responsiveness of the corpus luteum of the rhesus monkey (Macaca mulatta) to gonadotrophin in vitro during spontaneous and prostaglandin F2 -induced luteolysis

Reproduction ◽  
1995 ◽  
Vol 103 (1) ◽  
pp. 107-113 ◽  
Author(s):  
F. J. Auletta ◽  
L. B. Kelm ◽  
M. J. Schofield
Keyword(s):  
Rhesus Monkey ◽  
Corpus Luteum ◽  
Macaca Mulatta

65 BLASTOCYSTS DERIVED FROM ADULT FIBROBLASTS OF RHESUS MONKEY (MACACA MULATTA) USING INTERSPECIES SOMATIC CELL NUCLEAR TRANSFER

2010 ◽  
Vol 22 (1) ◽  
pp. 191
Author(s):  
D. K. Kwon ◽  
J. T. Kang ◽  
S. J. Park ◽  
M. N. L. Gomez ◽  
S. J. Kim ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Somatic Cell ◽  
Macaca Mulatta ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Developmental Rate ◽  
Cell Stage ◽  
Nuclear Number

Interspecies somatic cell nuclear transfer (iSCNT) has alternatively chosen in primate SCNT because of the difficulty in collecting enough oocytes for research. The purpose of this experiment is to produce iSCNT-derived blastocysts using enucleated cow (Bos taurus) metaphase II oocytes and adult rhesus monkey (Macaca mulatta) fibroblasts. Ear skin tissueofrhesus monkey (male, 6 years old) was collected by biopsy and fibroblasts were isolated. Immature COCs from cow ovaries were collected and matured in vitro in TCM-199. Squish enucleation was done in the presence of bisbenzimide and cytochalasin B. After enucleation, a single rhesus monkey somatic cell was injected into the perivitelline space of an enucleated oocyte through the slit in the zona pellucida made during enucleation. Subsequently, the rhesus monkey somatic cell and cow oocyte membranes were electrically fused. The nonactivated interspecies cloned couplets were cultured for 2 h to allow reprogramming to occur. Then, couplets were activated using a 2-step protocol consisting of treatment with 5 μM ionomycin for 4 to 5 min and subsequently with 2mM 6-DMAP for 4 h. Activated iSCNT embryos were cultured for 10 days inmodified SOF with various conditions (at 37 to39°C, 5 to 5.5% CO2 and 5 to 20% O2) to examine the effects ofIVC conditions. As a results, most embryos were arrested at the 8- to 16-cell stage and only 3 blastocysts were derived from rhesus monkey iSCNT. The blastocyst developmental rate was 0.26% generated from the total IVC activated interspecies embryos (n = 1153). Among the 3 blastocysts, 2 of them were used for counting nuclear number using bisbenzimide staining. The nuclear number of the 2 iSCNT-derived blastocysts was 51 and 24, respectively. The other iSCNT-derived blastocyst was used for analyzing mitochondrial (mt)DNAto confirm that it contained both cow and rhesus monkey mtDNA. As a result, mtDNA from both rhesus monkey and cow were detected inPCR analysis. The band intensity was more dominant for cow mtDNA than for rhesus monkey mtDNA. Although the blastocyst developmental rate is extremely low, it is confirmed that two phylogenetically distant species including primate could develop in vitro until the blastocyst stage by iSCNT. The in vitro developmental system of this rhesus monkey iSCNT-derived blastocysts provides a platform for further improvement of developmental rate and quality of rhesus monkey iSCNT-derived blastocysts. It also provides an opportunity to establish rhesus monkey iSCNT-derived embryonic stem cell lines for study of rhesus monkey nucleus and cow mitochondria interaction mechanisms during early developmental stages. This study was financially supported by the Korean MEST, through the BK21 program for Veterinary Science, and SNU foundation (Benefactor; RNL Bio).

Blastocysts derived from adult fibroblasts of a rhesus monkey (Macaca mulatta) using interspecies somatic cell nuclear transfer

Zygote ◽  
2011 ◽  
Vol 19 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Dae Kee Kwon ◽  
Jung Taek Kang ◽  
Sol Ji Park ◽  
Ma Ninia Limas Gomez ◽  
Su Jin Kim ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Somatic Cell ◽  
Macaca Mulatta ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Development Rate ◽  
Attractive Alternative ◽  
Cell Stage

SummaryIn non-human primates, it is difficult to collect sufficient numbers of oocytes for producing identical embryos by somatic cell nuclear transfer (SCNT). Because of this factor, inter-species SCNT (iSCNT) using heterospecific oocytes is an attractive alternative approach. The objective of this study was to produce iSCNT-derived blastocysts using enucleated cow (Bos taurus) metaphase II oocytes and adult rhesus monkey (Macaca mulatta) fibroblasts. Ear skin tissue from a 6-year-old male rhesus monkey was collected by biopsy and fibroblasts were isolated. Immature cumulus–oocyte complexes from cow ovaries were collected and matured in vitro in Medium 199. The enucleated oocytes were reconstructed with rhesus monkey fibroblasts and iSCNT embryos were cultured in modified synthetic oviduct fluid in an atmosphere of 5–5.5% CO2 under various conditions (37–39 °C and 5–20% O2) to examine the effects of in vitro culture conditions. Most embryos were arrested at the 8- or 16-cell stage and only three blastocysts were derived in this way using iSCNT from a total of 1153 cultured activated embryos (0.26% production rate). Two of the three blastocysts were used for counting nuclear numbers using bisbenzimide staining, which were 51 and 24. The other iSCNT-derived blastocyst was used to analyse mitochondrial DNA (mtDNA) by PCR, and both rhesus monkey and cow mtDNA were detected. Although the development rate was extremely low, this study established that iSCNT using two phylogenetically distant species, including a primate, could produce blastocysts. With improvements in the development rate, it may be possible to produce rhesus monkey iSCNT-derived embryonic stem cell lines for studies on primate nucleus and cow mitochondria interaction mechanisms.

PRODUCTION OF THROMBOXANE B2 BY INTRA-UTERINE TISSUES FROM LATE PREGNANT RHESUS MONKEYS (MACACA MULATTA) IN VITRO

1978 ◽  
Vol 79 (1) ◽  
pp. 103-106 ◽  
Author(s):  
M. D. MITCHELL ◽  
B. R. HICKS ◽  
G. D. THORBURN ◽  
J. S. ROBINSON
Keyword(s):  
Caesarean Section ◽  
Rhesus Monkey ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Late Pregnancy ◽  
Significant Trend ◽  
Thromboxane B2 ◽  
Decidua Basalis

The rates of production of thromboxane B2 in vitro by intra-uterine tissues obtained from late pregnant monkeys by Caesarean section have been determined. The general quantitative order of rates of production was decidua basalis = decidua parietalis > placenta > chorion > amnion = myometrium. Myometrial production of thromboxane B2 was greater at term than during late pregnancy; no other tissue showed a significant trend with advancing gestation. These data demonstrate that the production of thromboxane B2 by intra-uterine tissues from late pregnant monkeys is both qualitatively and quantitatively different from the production of prostaglandins described previously. It is suggested that prostaglandins rather than thromboxanes are more intimately involved in the onset of labour in the rhesus monkey.

In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta)

2011 ◽  
Vol 47 (5-6) ◽  
pp. 383-390 ◽  
Author(s):  
Shaohui Ji ◽  
Xihong Wang ◽  
Jianhong Shu ◽  
Aijing Sun ◽  
Wei Si ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Progenitor Cells ◽  
Macaca Mulatta ◽  
Epithelial Progenitor Cells

Expression of gelatinases and tissue inhibitors of metalloproteinases in the rhesus monkey (Macaca mulatta) corpus luteum

2001 ◽  
Vol 46 (15) ◽  
pp. 1292-1296 ◽  
Author(s):  
Qinglei Li ◽  
Hongmei Wang ◽  
Longjiang Shao ◽  
Xiaoguang Yu ◽  
Jiang Ni ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Corpus Luteum ◽  
Macaca Mulatta ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Tissue Inhibitors

Gonadotropin-Sensitive Progesterone Production by Rhesus Monkey Luteal Cells in Vitro: A Function of Age of the Corpus Luteum During the Menstrual Cycle

Endocrinology ◽  
1977 ◽  
Vol 100 (2) ◽  
pp. 506-512 ◽  
Author(s):  
RICHARD L. STOUFFER ◽  
WILBERT E. NIXON ◽  
BELA J. GULYAS ◽  
GARY D. HODGEN
Keyword(s):  
Menstrual Cycle ◽  
Rhesus Monkey ◽  
Corpus Luteum ◽  
Luteal Cells ◽  
Progesterone Production

A Tubal Pregnancy in a Rhesus Monkey (Macaca mulatta)

1982 ◽  
Vol 19 (3) ◽  
pp. 239-245 ◽  
Author(s):  
C. P. Jerome ◽  
A. G. Hendrickx
Keyword(s):  
Weight Loss ◽  
Rhesus Monkey ◽  
Corpus Luteum ◽  
Macaca Mulatta ◽  
Clinical Signs ◽  
Histological Section ◽  
Tubal Pregnancy ◽  
Uterine Tube

Tubal pregnancy was diagnosed on histological section of a nodule within the uterine tube of a multiparous rhesus monkey. Clinical signs were anorexia and weight loss. The tube contained a placenta of approximately 35 days gestation, but no recognizable embryo or amnion. The ipsilateral ovary contained a degenerating corpus luteum.

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