Organ-specific change inDolichos biflorus lectin binding by myocardial endothelial cells during in vitro cultivation

1993 ◽  
Vol 29 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Johanna Plendl ◽  
Laura Hartwell ◽  
Robert Auerbach
Keyword(s):  
Endothelial Cells ◽  
Lectin Binding ◽  
In Vitro Cultivation ◽  
Specific Change ◽  
Organ Specific

Assessment of cytocompatibility and mechanical properties of detergent-decellularized ovine pericardial tissue

2019 ◽  
Vol 42 (11) ◽  
pp. 628-635
Author(s):  
Alexandru Mogaldea ◽  
Karolina Theodoridis ◽  
Tobias Goecke ◽  
Igor Tudorache ◽  
Axel Haverich ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Endothelial Cells ◽  
Fluorescent Protein ◽  
Fluorescent Microscopy ◽  
Burst Pressure ◽  
In Vitro Cultivation ◽  
Detergent Treatment ◽  
Decellularized Tissue ◽  
Retention Strength

Background: Autologous pericardium is widely used for the repair of different sized cardiovascular defects. However, its use is limited especially in redo cardiac surgery. We developed an engineered tissue based on decellularized pericardium reseeded with blood-derived endothelial cells. Materials and Methods: Decellularization of ovine pericardium was performed using detergent treatment. Ovine outgrowth blood-derived and green fluorescent protein–labeled endothelial cells were used to reseed the decellularized ovine pericardium on the mesothelial side. The cell adhesion was assessed using fluorescent microscopy up to 15 days of in vitro cultivation. The mechanical properties of the pericardium were evaluated using suturability, burst pressure, and suture retention strength tests. Results: After decellularization the pericardial sheets appeared cell-free and repopulation using ovine blood-derived endothelial cells was successful by forming a robust monolayer. Detergent treatment did not affect the extracellular matrix. The thickness of decellularized tissue was similar to native ovine pericardium (285.3 ± 28.2 µm, respective 276.9 ± 23.8 µm, p = 0.48). Decellularized patch showed similar suturability comparable to the native ovine pericardium. Resulted burst pressure was not significantly different (native/decellularized: 312.5 ± 13.6/304.2 ± 16, p = 0.35). The suture retention strength of native pericardium was 638.33 ± 90.2 gr and comparable to decellularized tissue (622.2 ± 89.9 gr, p = 0.76). No differences were observed concerning elongation of native and decellularized pericardium (8.33 ± 1.5 and 8.5 ± 0.84 mm, respectively; p = 0.82). Conclusion: Mesothelial surface of decellularized ovine pericardium is suitable for reseeding with ovine blood-derived endothelial cells. The mechanical properties of detergent-treated pericardium were comparable to native tissue.

Biochemical characterization of some raptor trypanosomes. I. In vitro cultivation and lectin-binding studies

1986 ◽  
Vol 64 (1) ◽  
pp. 189-194 ◽  
Author(s):  
Carl E. Kirkpatrick ◽  
Cynthia A. Terway-Thompson
Keyword(s):  
Biochemical Characterization ◽  
Ricinus Communis ◽  
Lectin Binding ◽  
The Other ◽  
In Vitro Cultivation ◽  
Type I ◽  
Binding Studies ◽  
Binding Characteristics ◽  
American Kestrels

Nine trypanosome strains from five species of raptors were cultivated in vitro in a monophasic medium. Two morphologically distinct trypanosomes were observed in culture: those from American kestrels (Falco sparverius) were smaller than the other strains. The two kestrel (KT) trypanosome strains showed in vitro growth kinetics that differed from the larger trypanosomes, and the KT strains, unlike the others, required hemin in the medium for growth. The effectiveness of eight plant lectins to induce the agglutination of cultured trypanosomes was studied as a means of differentiating the various strains. It was found that lectins from Lens culinaris and Ricinus communis (type I) were particularly effective in distinguishing the KT strains from the other raptor trypanosome strains. Based on the results of experiments in which lectin-mediated trypanosome agglutination was inhibited by the addition of various monosaccharides, it is concluded that all of the avian trypanosomes studied express surface methyl α-D-mannoside, D(+)-galactose, and (or) α-lactose. Only the relatively large raptor trypanosome isolates expressed N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, and α-L(−)-fucose on their surfaces. The differences in lectin-binding characteristics between the two morphologic types of raptor trypanosome were as great as those among each of the avian trypanosomes and the mammalian trypanosomatids Leishmania chagasi and Trypanosoma rhodesiense.

In vitro cultivation of Anaplasma marginale in bovine erythrocytes co-cultured with endothelial cells

1997 ◽  
Vol 73 (1-2) ◽  
pp. 43-52 ◽  
Author(s):  
S.D. Waghela ◽  
D. Cruz ◽  
R.E. Droleskey ◽  
J.R. DeLoach ◽  
G.G. Wagner
Keyword(s):  
Endothelial Cells ◽  
Anaplasma Marginale ◽  
In Vitro Cultivation ◽  
Bovine Erythrocytes

Abstract 44: Organ-specific Stochastic Phenotype Switching is Required for Endothelial Health

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Lei Yuan ◽  
Gary C Chan ◽  
David Beeler ◽  
Lauren Janes ◽  
Katherine C Spokes ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Damage ◽  
Bet Hedging ◽  
Organ Specific ◽  
Capillary Endothelial Cells ◽  
Unicellular Organisms ◽  
Phenotype Switching ◽  
Von Willebrand ◽  
Willebrand Factor

Among unicellular organisms, stochastic phenotype switching is a documented strategy for survival. These populations "hedge their bets": while the majority of their cells are adapted to their present environment, a minority remains poised to thrive under drastically different conditions. Bet hedging has also been described in metazoan cells, primarily in vitro. However, its role in tissue homeostasis has yet to be established. Here, we show that von Willebrand factor (vWF) is expressed in a spatially heterogeneous manner in a small fraction of capillary endothelial cells in the heart, skeletal muscle, lung and brain. Moreover, these mosaic patterns are dynamic, in that vWF expression stochastically toggles ON/OFF over time. By contrast, expression of vWF in the aorta and liver is static in time. In cultured primary endothelial cells, biological noise resulted in mosaic vWF heterogeneity through a promoter-level DNA methylation switch. Finally, vWF-/- mice demonstrated extensive endothelial cell damage in capillaries of the heart and impaired cardiac function, but not kidney or aorta. Taken together, these findings suggest that dynamic mosaicism of vWF expression is functionally relevant and that bet hedging represents a previously unrecognized strategy for adaptive, organ-specific homeostasis.

In vitro cultivation of the vascular phase of Sarcocystis hirsuta (Apicomplexa)

1990 ◽  
Vol 68 (5) ◽  
pp. 1068-1070 ◽  
Author(s):  
R. J. Cawthorn ◽  
R. J. F. Markham ◽  
N. D. Hitt ◽  
D. Despres
Keyword(s):  
Endothelial Cells ◽  
Pulmonary Artery ◽  
In Vitro Cultivation ◽  
Sarcocystis Hirsuta

Sporozoites of Sarcocystis hirsuta (Apicomplexa), although they also penetrated into bovine monocytes, developed to the schizogonous phase only in bovine pulmonary artery endothelial cells. Schizonts were evident beginning 14 days after sporozoite inoculation (DAI) and persisted to 62 DAI, when experiments were terminated. Merozoites and schizonts were most numerous 35–53 DAI. The number of schizogonous generations was not determined. In vitro cultivation of schizonts of S. hirsuta will facilitate comparisons with development of S. cruzi, and this will aid elucidation of mechanisms of pathogenesis and immunologic responses caused by these two important cattle parasites.

scholarly journals Plasmodium chabaudi-Infected Erythrocytes Adhere to CD36 and Bind to Microvascular Endothelial Cells in an Organ-Specific Way

2000 ◽  
Vol 68 (7) ◽  
pp. 4135-4144 ◽  
Author(s):  
Maria M. Mota ◽  
William Jarra ◽  
Elizabeth Hirst ◽  
Pradeep K. Patnaik ◽  
Anthony A. Holder
Keyword(s):  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
In Vivo Model ◽  
Dependent Manner ◽  
Plasmodium Chabaudi ◽  
Major Barrier ◽  
Organ Specific ◽  
Microvascular Endothelial

ABSTRACT Adherence of erythrocytes infected with Plasmodium falciparum to microvascular endothelial cells (sequestration) is considered to play an important role in parasite virulence and pathogenesis. However, the real importance of sequestration for infection and disease has never been fully assessed. The absence of an appropriate in vivo model for sequestration has been a major barrier. We have examined the rodent malaria parasite Plasmodium chabaudi chabaudi AS in mice as a potential model. Erythrocytes infected with this parasite adhere in vitro to purified CD36, a critical endothelium receptor for binding P. falciparum-infected erythrocytes. P. c. chabaudi-infected erythrocytes adhere in vitro to endothelial cells in a gamma interferon-dependent manner, suggesting the involvement of additional adhesion molecules in the binding process, as is also the case with P. falciparum-infected cells. Furthermore, plasma or sera from infected and hyperimmune mice, respectively, have the ability to block binding of infected erythrocytes to endothelial cells. In vivo, erythrocytes containing mature P. c. chabaudi parasites are sequestered from the peripheral circulation. Sequestration is organ specific, occurring primarily in the liver, although intimate contact between infected erythrocytes and endothelial cells is also observed in the spleen and brain. The results are discussed in the context of the use of this model to study (i) the relationship between endothelial cell activation and the level of sequestration and (ii) the primary function of sequestration in malaria infection.

scholarly journals Differentiation of human intestinal organoids with endogenous vascular endothelial cells

2020 ◽  
Author(s):  
Emily M. Holloway ◽  
Joshua H. Wu ◽  
Michael Czerwinkski ◽  
Caden W. Sweet ◽  
Angeline Wu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Specific Gene ◽  
Gene Signatures ◽  
Transcriptional Signature ◽  
Intestinal Organoids ◽  
Organ Systems ◽  
Organ Specific ◽  
And Function

SUMMARYHuman pluripotent stem cell (hPSC)-derived intestinal organoids (HIOs) generated using directed differentiation lack some cellular populations found in the native organ, including vasculature. Using single cell RNA sequencing (scRNAseq), we have identified a transient population of endothelial cells (ECs) present early in HIO differentiation that are lost over time in culture. Here, we have developed a method to enhance co-differentiation and maintenance of ECs within HIOs (vHIOs). Given that ECs are known to possess organ specific gene expression, morphology and function, we used bulk RNAseq and scRNAseq to interrogate the developing human intestine, lung, and kidney in order to identify organ-enriched EC-gene signatures in these organ systems. By comparing organ-specific gene signatures along with markers validated by fluorescent in situ hybridization to HIO ECs, we find that HIO ECs grown in vitro share the highest similarity with native intestinal ECs relative to kidney and lung. Together, these data show that HIOs can co-differentiate a native EC population that are properly patterned with an intestine-specific EC transcriptional signature in vitro.

Ultrastructural studies on the interaction of haemophilus influenzae with human endothelial cells in vitro

1990 ◽  
Vol 48 (3) ◽  
pp. 636-637
Author(s):  
D.J.P. Ferguson ◽  
M. Virji ◽  
H. Kayhty ◽  
E.R. Moxon
Keyword(s):  
Endothelial Cells ◽  
Haemophilus Influenzae ◽  
Intercellular Junctions ◽  
Blood Stream ◽  
Ultrastructural Studies ◽  
Endothelial Barriers ◽  
Serotype B ◽  
Meningitis In Children ◽  
Respiratory Epithelial

Haemophilus influenzae is a human pathogen which causes meningitis in children. Systemic H. influenzae infection is largely confined to encapsulated serotype b organisms and is a major cause of meningitis in the U.K. and elsewhere. However, the pathogenesis of the disease is still poorly understood. Studies in the infant rat model, in which intranasal challenge results in bacteraemia, have shown that H. influenzae enters submucosal tissues and disseminates to the blood stream within minutes. The rapidity of these events suggests that H. influenzae penetrates both respiratory epithelial and endothelial barriers with great efficiency. It is not known whether the bacteria penetrate via the intercellular junctions, are translocated within the cells or carried across the cellular barrier in 'trojan horse' fashion within phagocytes. In the present studies, we have challenged cultured human umbilical cord_vein endothelial cells (HUVECs) with both capsulated (b+) and capsule-deficient (b-) isogenic variants of one strain of H. influenzae in order to investigate the interaction between the bacteria and HUVEC and the effect of the capsule.

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