Fed-batch culture ofEscherichia coli W by exponential feeding of sucrose as a carbon source

1997 ◽  
Vol 11 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Jeongseok Lee ◽  
Sang Yup Lee ◽  
Sunwon Park
Keyword(s):  
Carbon Source ◽  
Batch Culture ◽  
Ofescherichia Coli ◽  
Fed Batch ◽  
Exponential Feeding ◽  
Fed Batch Culture

Independent Exponential Feeding of Glycerol and Methanol for Fed-Batch Culture of Recombinant Hansenula polymorpha DL-1

2003 ◽  
Vol 111 (2) ◽  
pp. 65-80 ◽  
Author(s):  
H. Moon ◽  
S. W. Kim ◽  
J. Lee ◽  
S. K. Rhee ◽  
E. S. Choi ◽  
...  
Keyword(s):  
Batch Culture ◽  
Hansenula Polymorpha ◽  
Fed Batch ◽  
Exponential Feeding ◽  
Fed Batch Culture

Fed-batch culture of hairy root using fructose as a carbon source

1991 ◽  
Vol 72 (6) ◽  
pp. 457-460 ◽  
Author(s):  
Nobuyuki Uozumi ◽  
Katsuhito Kohketsu ◽  
Osamu Kondo ◽  
Hiroyuki Honda ◽  
Takeshi Kobayashi
Keyword(s):  
Carbon Source ◽  
Batch Culture ◽  
Hairy Root ◽  
Fed Batch ◽  
Fed Batch Culture

Yield Production of Recombinant Plasmid DNA withEscherichia Coliin Fed-Batch Culture by Pseudo-Exponential Feeding

2013 ◽  
Vol 27 (2) ◽  
pp. 3709-3715 ◽  
Author(s):  
Mounir M. Salem-Bekhit ◽  
Mohsen Bayomi ◽  
Fares Al-Anazi ◽  
Hesham Radawan
Keyword(s):  
Batch Culture ◽  
Plasmid Dna ◽  
Recombinant Plasmid ◽  
Fed Batch ◽  
Exponential Feeding ◽  
Yield Production ◽  
Fed Batch Culture

scholarly journals Bacterial Cellulose Synthesis by Gluconacetobacter xylinus: Enhancement via Fed-batch Fermentation Strategies in Glycerol Media

2021 ◽  
Vol 18 (22) ◽  
pp. 453
Author(s):  
Azila Adnan ◽  
Giridhar Nair ◽  
Mark Lay ◽  
Janis Swan
Keyword(s):  
Carbon Source ◽  
Bacterial Cellulose ◽  
Batch Culture ◽  
Batch Fermentation ◽  
Operation Mode ◽  
Volumetric Productivity ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Pulse Feed ◽  
Fed Batch Culture

Bacterial cellulose (BC) is an abundant polysaccharide, which is secreted by several genera of bacteria. It has remarkable characteristics, which include high purity, high tensile strength, high biocompatibility and non-toxic. The main feature that differentiates BC and plant cellulose (PC) is the absence of contaminants such as lignin, hemicellulose and pectin. However, the main drawbacks in producing BC are low yield and expensive carbon source. Due to that, this study was carried out to enhance BC volumetric productivity in fed-batch operation mode using glycerol as a carbon source. BC was produced in fill-and-draw and pulse-feed fed-batch cultures of Gluconacetobacter xylinus DSM 46604 in a 3-L bench-top bioreactor. The fed-batch fermentation trials were conducted in agitated and aerobic conditions at 30 ºC. For fill-and-draw fed-batch culture, a total of 24.2 g/L of BC accumulated in the bioreactor after 9 days, which corresponded to a yield and productivity of 0.2 g/g and 2.69 g/L/day, respectively. Pulse-feed fed-batch fermentation resulted in a yield and volumetric productivity of 0.38 g/g and 2.71 g/L/day, respectively. The pulse-feed fed-batch culture proved to be a better fermentation system for utilizing glycerol, which is a low-cost and abundant carbon source. HIGHLIGHTS Komagataeibacter species, which were formerly known as Acetobacter or Gluconacetobacter is one of the Gram-negative BC producers that secretes a large quantity of BC microfibrils extracellularly One of the main challenges in bacterial cellulose (BC) production is low productivity and high processing cost As fed-batch fermentation is one of the operation modes in bioprocess that can control the microbial growth rate, this operation mode is conducted to enhance the yield of BC, substrate consumption and also volumetric productivity Fill-and-draw and pulse feed fed-batch culture were conducted to enhance yield and volumetric productivity. The pulse-feed fed-batch culture resulted to be a favorable operation mode for utilizing glycerol, which is a low-cost and abundant carbon source GRAPHICAL ABSTRACT

High-Density pH-Auxostat Fed-Batch Culture of Schizochytrium limacinum SR21 with Acetic Acid as a Carbon Source

2020 ◽  
Vol 192 (4) ◽  
pp. 1163-1175
Author(s):  
Muhammad Shafiq ◽  
Liaqat Zeb ◽  
Guannan Cui ◽  
Muhammad Jawad ◽  
Zhanyou Chi
Keyword(s):  
Acetic Acid ◽  
Carbon Source ◽  
Batch Culture ◽  
High Density ◽  
Fed Batch ◽  
Fed Batch Culture ◽  
Schizochytrium Limacinum
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