Fed-batch culture of insect cells with exponential feeding of amino acid and yeastolate solution

2000 ◽  
Vol 23 (4) ◽  
pp. 367-370 ◽  
Author(s):  
J. H. Kim ◽  
E. J. Kim ◽  
T. H. Park
Keyword(s):  
Amino Acid ◽  
Batch Culture ◽  
Insect Cells ◽  
Fed Batch ◽  
Exponential Feeding ◽  
Fed Batch Culture

Independent Exponential Feeding of Glycerol and Methanol for Fed-Batch Culture of Recombinant Hansenula polymorpha DL-1

2003 ◽  
Vol 111 (2) ◽  
pp. 65-80 ◽  
Author(s):  
H. Moon ◽  
S. W. Kim ◽  
J. Lee ◽  
S. K. Rhee ◽  
E. S. Choi ◽  
...  
Keyword(s):  
Batch Culture ◽  
Hansenula Polymorpha ◽  
Fed Batch ◽  
Exponential Feeding ◽  
Fed Batch Culture

Optimization of a feed medium for fed-batch culture of insect cells using a genetic algorithm

2002 ◽  
Vol 81 (3) ◽  
pp. 269-278 ◽  
Author(s):  
R. C. L. Marteijn ◽  
O. Jurrius ◽  
J. Dhont ◽  
C. D. de Gooijer ◽  
J. Tramper ◽  
...  
Keyword(s):  
Genetic Algorithm ◽  
Batch Culture ◽  
Insect Cells ◽  
Fed Batch ◽  
Feed Medium ◽  
Fed Batch Culture

scholarly journals Reduced Folate Supply as a Key to Enhanced l-Serine Production by Corynebacterium glutamicum

2006 ◽  
Vol 73 (3) ◽  
pp. 750-755 ◽  
Author(s):  
Michael Stolz ◽  
Petra Peters-Wendisch ◽  
Helga Etterich ◽  
Tanja Gerharz ◽  
Robert Faurie ◽  
...  
Keyword(s):  
Amino Acid ◽  
Corynebacterium Glutamicum ◽  
Batch Culture ◽  
Serine Hydroxymethyltransferase ◽  
Dependent Manner ◽  
Fed Batch ◽  
Microbial Process ◽  
Expression Control ◽  
Fed Batch Culture ◽  
Dose Dependent

ABSTRACT The amino acid l-serine is required for pharmaceutical purposes, and the availability of a sugar-based microbial process for its production is desirable. However, a number of intracellular utilization routes prevent overproduction of l-serine, with the essential serine hydroxymethyltransferase (SHMT) (glyA) probably occupying a key position. We found that constructs of Corynebacterium glutamicum strains where chromosomal glyA expression is dependent on P tac and lacI Q are unstable, acquiring mutations in lacI Q, for instance. To overcome the inconvenient glyA expression control, we instead considered controlling SHMT activity by the availability of 5,6,7,8-tetrahydrofolate (THF). The pabAB and pabC genes of THF synthesis were identified and deleted in C. glutamicum, and the resulting strains were shown to require folate or 4-aminobenzoate for growth. Whereas the C. glutamicum ΔsdaA strain (pserACB) accumulates only traces of l-serine, with the C. glutamicum ΔpabABCΔsdaA strain (pserACB), l-serine accumulation and growth responded in a dose-dependent manner to an external folate supply. At 0.1 mM folate, 81 mM l-serine accumulated. In a 20-liter controlled fed-batch culture, a 345 mM l-serine accumulation was achieved. Thus, an efficient and highly competitive process for microbial l-serine production is available.

Sign in / Sign up

Export Citation Format

Share Document