Biotransformation of trichloroethene by pure bacterial cultures

Abstract Activated sludge samples from two Toronto sewage treatment plants were subjected to the extraction of exocellular material by means of 9 different methods suggested for this purpose. Some of those methods, originally developed for pure bacterial cultures, were modified for the application to activated sludge. The amount of exocellular material obtained varied for Lakeview sludges from 0.4 to 3.2% of their dry volatile solids, and for Humber sludges from 0.3 to 5.3%. It has been found that extractions by the use of sulphuric acid, high-speed centrifugation and sodium hydroxide, were not suitable for the studied material. Especially surprising was the ineffectiveness of high-speed centrifugation to yield any measurable amounts of extract. The boiling water extraction is recommended for further studies on activated sludge exocellular material. The material extracted from activated sludge is very complex in nature. Generally more polysaccharide than protein was extracted, but the remaining volatile material may form up to 70% of the dry weight.

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PCBs and PAHs Restrain the Use of Sludge as a Renewable Resource

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1001.162 ◽

2014 ◽

Vol 1001 ◽

pp. 162-170

Author(s):

Radmila Kučerová ◽

Tomáš Sezima ◽

Eugen Sikora ◽

Ivana Truxová ◽

Lucie Kučerová ◽

...

Keyword(s):

Sewage Sludge ◽

Polychlorinated Biphenyls ◽

Laboratory Experiment ◽

Pseudomonas Putida ◽

Polyaromatic Hydrocarbons ◽

Renewable Resource ◽

Bacterial Cultures ◽

Pure Bacterial Cultures

The aim of this research is to reduce the quantities of polyaromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) in the samples of non-hygienized sewage sludge via laboratory biodegradation. Pure bacterial cultures of Pseudomonas putida, Rhodococcus sp. and their mixture in 1:1 proportion have been used. The laboratory experiment lasted for 28 days and the acquired values were compared with Decree 294/2005 Coll. The obtained results imply that biodegradation of such contaminated samples is practicable. Using the bacterial mixture, over 85 % Σ of PCBs were degraded, and applying Rhodococcus sp. as much as 95.86 % of the original concentration of PAHs were removed.

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Examination of enzymes concentration in the blood of rats with sepsis caused by mixed and pure bacterial cultures

Acta veterinaria ◽

10.2298/avb1306609s ◽

2013 ◽

Vol 63 (5-6) ◽

pp. 609-620

Author(s):

Dragica Stojanovic ◽

Zorana Kovacevic ◽

Marina Zekic ◽

Jelena Asanin ◽

R. Ratajac ◽

...

Keyword(s):

Bacterial Cultures ◽

Pure Bacterial Cultures

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Improved XLT4 Agar: Small Addition of Peptone to Promote Stronger Production of Hydrogen-Sulfide by Salmonellae

Journal of Food Protection ◽

10.4315/0362-028x-57.10.854 ◽

1994 ◽

Vol 57 (10) ◽

pp. 854-858 ◽

Cited By ~ 3

Author(s):

R. G. MILLER ◽

C. R. TATE ◽

E. T. MALLINSON

Keyword(s):

Hydrogen Sulfide ◽

Clinical Samples ◽

Pork Sausage ◽

Bacterial Cultures ◽

Proteose Peptone ◽

Low Concentrations ◽

Production Of Hydrogen ◽

Hydrogen Sulfide Production ◽

Selective Plating ◽

Pure Bacterial Cultures

Xylose lysine tergitol4 agar (XLT4) is a highly selective plating medium used for isolating salmonellae. Studies have shown that XLT4 increases the recovery of salmonellae found in food, environmental and clinical samples. Further testing demonstrated that the addition of low concentrations of proteose peptone No. 3 (pp3) to XLT4 produced blacker Salmonella colonies in shorter incubation times (increased hydrogen sulfide production), while still maintaining strong inhibition of competing bacteria. The increased black colony formation facilitates prompt recognition of the weaker hydrogen sulfide-producing Salmonella strains. Test concentrations of pp3 at 0.5, 1.2 and 1.8 g/L were added to XLT4 and compared with plain XLT4 using pure bacterial cultures. In addition, these four plating media, plus xylose lysine desoxycholate agar (XLD) were evaluated using nonspiked chicken liver and pork sausage samples. The concentration of 1.2 g/L of pp3 in XLT4 gave the best overall results. In virtually all cases, the Salmonella colonies were larger and more black than on plain XLT4 without pp3. The improved XLT4 is recommended for more reliable detection of salmonellae from food, environmental and clinical samples.

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An assessment of the quality and yield of flax fiber produced by the use of pure bacterial cultures in flax rets

Enzyme and Microbial Technology ◽

10.1016/0141-0229(92)90170-s ◽

1992 ◽

Vol 14 (2) ◽

pp. 131-134 ◽

Cited By ~ 8

Author(s):

J.A. Donaghy ◽

J.H. Boomer ◽

R.W. Haylock

Keyword(s):

Flax Fiber ◽

Bacterial Cultures ◽

Pure Bacterial Cultures

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Improved XLT4 Agar: Small Addition of Peptone to Promote Stronger Production of Hydrogen-Sulfide by Salmonellae

Journal of Food Protection ◽

10.4315/0362-028x-58.1.115 ◽

1995 ◽

Vol 58 (1) ◽

pp. 115-119 ◽

Cited By ~ 4

Author(s):

R. G. MILLER ◽

C. R. TATE ◽

E. T. MALLINSON

Keyword(s):

Hydrogen Sulfide ◽

Clinical Samples ◽

Pork Sausage ◽

Bacterial Cultures ◽

Proteose Peptone ◽

Low Concentrations ◽

Production Of Hydrogen ◽

Hydrogen Sulfide Production ◽

Selective Plating ◽

Pure Bacterial Cultures

Xylose lysine tergitol4 agar (XLT4) is a highly selective plating medium used for isolating salmonellae. Studies have shown that XLT4 increases the recovery of salmonellae found in food, environmental and clinical samples. Further testing demonstrated that the addition of low concentrations of proteose peptone No. 3 (pp3) to XLT4 produced blacker Salmonella colonies in shorter incubation times (increased hydrogen sulfide production), while still maintaining strong inhibition of competing bacteria. The increased black colony formation facilitates prompt recognition of the weaker hydrogen sulfide-producing Salmonella strains. Test concentrations of pp3 at 0.5, 1.2 and 1.8 g/L were added to XLT4 and compared with plain XLT4 using pure bacterial cultures. In addition, these four plating media, plus xylose lysine desoxycholate agar (XLD) were evaluated using nonspiked chicken liver and pork sausage samples. The concentration of 1.2 g/L of pp3 in XLT4 gave the best overall results. In virtually all cases, the Salmonella colonies were larger and more black than on plain XLT4 without pp3. The improved XLT4 is recommended for more reliable detection of salmonellae from food, environmental and clinical samples.

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Enantioselective degradation of the herbicide mecoprop [2-(2-methyl-4-chlorophenoxy) propionic acid] by mixed and pure bacterial cultures

FEMS Microbiology Ecology ◽

10.1111/j.1574-6941.1994.tb00105.x ◽

1994 ◽

Vol 14 (3) ◽

pp. 191-199 ◽

Cited By ~ 53

Author(s):

V.A. Tett ◽

A.J. Willetts ◽

H.M. Lappin-Scott

Keyword(s):

Propionic Acid ◽

Bacterial Cultures ◽

Enantioselective Degradation ◽

Pure Bacterial Cultures

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Degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures

Applied Microbiology and Biotechnology ◽

10.1007/bf00903787 ◽

1990 ◽

Vol 32 (4) ◽

pp. 479-484 ◽

Cited By ~ 140

Author(s):

Walter D. Weissenfels ◽

Michael Beyer ◽

J�rgen Klein

Keyword(s):

Bacterial Cultures ◽

Pure Bacterial Cultures

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Impact of low-concentrated acidic electrolysed water obtained by membrane electrolysis on the decontamination of meat microbiota

Bulletin of the Veterinary Institute in Pulawy ◽

10.1515/bvip-2015-0055 ◽

2015 ◽

Vol 59 (3) ◽

pp. 369-376 ◽

Cited By ~ 3

Author(s):

Ewa Brychcy ◽

Andrzej Jarmoluk ◽

Krzysztof Marycz

Keyword(s):

Micrococcus Luteus ◽

Bactericidal Effect ◽

Plate Count ◽

E Coli ◽

Log Reduction ◽

Bacterial Cultures ◽

Number Of Microorganisms ◽

Plate Count Method ◽

Bacterial Cell Morphology ◽

Pure Bacterial Cultures

AbstractThe influence of acidic electrolysed water (AEW) treatment on inactivation of pure bacterial cultures inoculated onto the surface of agarised media and surface microbiota of pork meat were examined. Low-concentrated AEW (low concentration of sodium chloride and low current electrolysis) was generated by electrolysis (5 or 10 min) of 0.001% or 0.01% NaCl solution. The number of viable microorganisms was determined using a plate count method. The effect of AEW on bacterial cell morphology were investigated using scanning electron microscopy (SEM). After treatment with AEW, a significant, about 3.00 log reduction ofPseudomonas fluorescens, Yersinia enterocolitica, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Listeria monocytogenes,andMicrococcus luteuspopulations was observed. In the AEW treatment of pork, the highest reduction of total number of microorganisms (2.1 log reduction), yeast and moulds (2.5-2.6 log reduction), and psychrotrophs (more than 1 log reduction) was observed after spraying with 0.001% NaCl subjected to 10 min electrolysis. SEM revealed disruption and lysis ofE. coliandS. aureuscells treated with AEW, suggesting a bactericidal effect. Higher available chlorine concentration (0.37-8.45 mg/L), redox potential (863.1-1049.8 mV), and lower pH (2.73-3.70) had an influence on the shape of bacteria and the number of breaks in the bacterial membrane.

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