Biohydrogen fermentation from Pistia stratiotes (aquatic weed) using mixed and pure bacterial cultures

2019 ◽  
Vol 44 (33) ◽  
pp. 17720-17731 ◽  
Author(s):  
Nonsikelelo Precios Mthethwa ◽  
Mahmoud Nasr ◽  
Sammy Lewis Kiambi ◽  
Faizal Bux ◽  
Sheena Kumari
Keyword(s):  
Pistia Stratiotes ◽  
Aquatic Weed ◽  
Bacterial Cultures ◽  
Pure Bacterial Cultures

Activated Sludge Exocellular Material Extraction Methods and Problems

1974 ◽  
Vol 9 (1) ◽  
pp. 250-261
Author(s):  
D.F. Carr ◽  
J. Ganczarczyk
Keyword(s):  
Activated Sludge ◽  
High Speed ◽  
Sewage Treatment ◽  
Dry Weight ◽  
Extraction Methods ◽  
Volatile Material ◽  
Bacterial Cultures ◽  
Volatile Solids ◽  
Pure Bacterial Cultures ◽  
Material Extraction

Abstract Activated sludge samples from two Toronto sewage treatment plants were subjected to the extraction of exocellular material by means of 9 different methods suggested for this purpose. Some of those methods, originally developed for pure bacterial cultures, were modified for the application to activated sludge. The amount of exocellular material obtained varied for Lakeview sludges from 0.4 to 3.2% of their dry volatile solids, and for Humber sludges from 0.3 to 5.3%. It has been found that extractions by the use of sulphuric acid, high-speed centrifugation and sodium hydroxide, were not suitable for the studied material. Especially surprising was the ineffectiveness of high-speed centrifugation to yield any measurable amounts of extract. The boiling water extraction is recommended for further studies on activated sludge exocellular material. The material extracted from activated sludge is very complex in nature. Generally more polysaccharide than protein was extracted, but the remaining volatile material may form up to 70% of the dry weight.

scholarly journals GC-MS ANALYSIS OF INVASIVE AQUATIC WEED, PISTIA STRATIOTES L. AND EICHHORNIA CRASSIPES (MART.) SOLMS

2017 ◽  
Vol 9 (3) ◽  
pp. 111 ◽  
Author(s):  
Tulika Tyagi ◽  
Mala Agarwal
Keyword(s):  
Methyl Ester ◽  
Ethyl Ester ◽  
Eichhornia Crassipes ◽  
Acid Methyl Ester ◽  
Acid Ethyl Ester ◽  
Pistia Stratiotes ◽  
Aquatic Weed ◽  
Hexadecenoic Acid ◽  
Hexadecanoic Acid ◽  
Acid Methyl

Objective: To investigate the bioactive components of an invasive aquatic weed, Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms vegetative parts by using Gas Chromatography-Mass Spectrometer (GC-MS).Methods: The chemical compositions of the ethanol extract of whole plant Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms was investigated using Agilent Technologies GC-MS (GC-7890A, MS 5975C).Results: The results of GC-MS analysis of the ethanolic extract revealed the existence of 28 phytochemical compounds in Pistia stratiotes L. n-Hexadecanoic acid,-11-Hexadecenoic acid, ethyl ester, Hexadecanoic acid, ethyl ester, Octadecanoic acid, ethyl ester, 2-Cyclopenten-1-one, 5-hydroxy-2,3-dimethyl, L-Glutamine, 2-Pentadecanone, 6,10,14-trimethyl, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, methyl ester,(Z,Z,Z), Nonadecane, 12,15-Octadecadiynoic acid, methyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, Diisooctyl phthalate, Docosanoic acid, ethyl ester, Stigmasterol, Bis(2-ethylhexyl) phthalate, 1-Monolinoleoylglycerol trimethylsilyl ether, Ethyl iso-allocholate are the major compound.The ethanolic extract of Eichhornia crassipes (Mart.) Solms contains 43 phytochemical compounds of high and low molecular weight n-Hexadecanoic acid, E-11-Hexadecenoic acid, ethyl ester, Palmitic acid, Phytol, 9,12,15-Octadecatrienal, 9,12-Octadecadienoic acid, ethyl ester, Linolenic acid, ethyl ester, Stearic acid, ethyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, α-Glyceryl linolenate, 1-Monolinoleoylglycerol trimethylsilyl ether, Linoleic acid, 2,3-bis-(O-TMS)-propyl ester, Stigmasterol, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z), Ethyl iso-allocholate, Cholesta-22,24-dien-5-ol, 4,4-dimethyl are the major compounds.These results indicates Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms possess potent antioxidant, anti-inflammatory, anticancer, antitumour, antiarthritic, cancer preventive, antibacterial effects so can be recommended as a plant of phytopharmaceutical importance.Conclusion: The ethanol extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms proves as a potential source of bioactive compounds of pharmacological importance.

PCBs and PAHs Restrain the Use of Sludge as a Renewable Resource

2014 ◽  
Vol 1001 ◽  
pp. 162-170
Author(s):  
Radmila Kučerová ◽  
Tomáš Sezima ◽  
Eugen Sikora ◽  
Ivana Truxová ◽  
Lucie Kučerová ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Polychlorinated Biphenyls ◽  
Laboratory Experiment ◽  
Pseudomonas Putida ◽  
Polyaromatic Hydrocarbons ◽  
Renewable Resource ◽  
Bacterial Cultures ◽  
Pure Bacterial Cultures

The aim of this research is to reduce the quantities of polyaromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) in the samples of non-hygienized sewage sludge via laboratory biodegradation. Pure bacterial cultures of Pseudomonas putida, Rhodococcus sp. and their mixture in 1:1 proportion have been used. The laboratory experiment lasted for 28 days and the acquired values were compared with Decree 294/2005 Coll. The obtained results imply that biodegradation of such contaminated samples is practicable. Using the bacterial mixture, over 85 % Σ of PCBs were degraded, and applying Rhodococcus sp. as much as 95.86 % of the original concentration of PAHs were removed.

Biotransformation of trichloroethene by pure bacterial cultures

2002 ◽  
Vol 47 (5) ◽  
pp. 467-472
Author(s):  
J. Růžička ◽  
J. Müller ◽  
D. Vít ◽  
V. Hutěčka ◽  
J. Hoffmann ◽  
...  
Keyword(s):  
Bacterial Cultures ◽  
Pure Bacterial Cultures

scholarly journals Examination of enzymes concentration in the blood of rats with sepsis caused by mixed and pure bacterial cultures

2013 ◽  
Vol 63 (5-6) ◽  
pp. 609-620
Author(s):  
Dragica Stojanovic ◽  
Zorana Kovacevic ◽  
Marina Zekic ◽  
Jelena Asanin ◽  
R. Ratajac ◽  
...  
Keyword(s):  
Bacterial Cultures ◽  
Pure Bacterial Cultures

Improved XLT4 Agar: Small Addition of Peptone to Promote Stronger Production of Hydrogen-Sulfide by Salmonellae

1994 ◽  
Vol 57 (10) ◽  
pp. 854-858 ◽  
Author(s):  
R. G. MILLER ◽  
C. R. TATE ◽  
E. T. MALLINSON
Keyword(s):  
Hydrogen Sulfide ◽  
Clinical Samples ◽  
Pork Sausage ◽  
Bacterial Cultures ◽  
Proteose Peptone ◽  
Low Concentrations ◽  
Production Of Hydrogen ◽  
Hydrogen Sulfide Production ◽  
Selective Plating ◽  
Pure Bacterial Cultures

Xylose lysine tergitol4 agar (XLT4) is a highly selective plating medium used for isolating salmonellae. Studies have shown that XLT4 increases the recovery of salmonellae found in food, environmental and clinical samples. Further testing demonstrated that the addition of low concentrations of proteose peptone No. 3 (pp3) to XLT4 produced blacker Salmonella colonies in shorter incubation times (increased hydrogen sulfide production), while still maintaining strong inhibition of competing bacteria. The increased black colony formation facilitates prompt recognition of the weaker hydrogen sulfide-producing Salmonella strains. Test concentrations of pp3 at 0.5, 1.2 and 1.8 g/L were added to XLT4 and compared with plain XLT4 using pure bacterial cultures. In addition, these four plating media, plus xylose lysine desoxycholate agar (XLD) were evaluated using nonspiked chicken liver and pork sausage samples. The concentration of 1.2 g/L of pp3 in XLT4 gave the best overall results. In virtually all cases, the Salmonella colonies were larger and more black than on plain XLT4 without pp3. The improved XLT4 is recommended for more reliable detection of salmonellae from food, environmental and clinical samples.

Improved XLT4 Agar: Small Addition of Peptone to Promote Stronger Production of Hydrogen-Sulfide by Salmonellae

1995 ◽  
Vol 58 (1) ◽  
pp. 115-119 ◽  
Author(s):  
R. G. MILLER ◽  
C. R. TATE ◽  
E. T. MALLINSON
Keyword(s):  
Hydrogen Sulfide ◽  
Clinical Samples ◽  
Pork Sausage ◽  
Bacterial Cultures ◽  
Proteose Peptone ◽  
Low Concentrations ◽  
Production Of Hydrogen ◽  
Hydrogen Sulfide Production ◽  
Selective Plating ◽  
Pure Bacterial Cultures

Xylose lysine tergitol4 agar (XLT4) is a highly selective plating medium used for isolating salmonellae. Studies have shown that XLT4 increases the recovery of salmonellae found in food, environmental and clinical samples. Further testing demonstrated that the addition of low concentrations of proteose peptone No. 3 (pp3) to XLT4 produced blacker Salmonella colonies in shorter incubation times (increased hydrogen sulfide production), while still maintaining strong inhibition of competing bacteria. The increased black colony formation facilitates prompt recognition of the weaker hydrogen sulfide-producing Salmonella strains. Test concentrations of pp3 at 0.5, 1.2 and 1.8 g/L were added to XLT4 and compared with plain XLT4 using pure bacterial cultures. In addition, these four plating media, plus xylose lysine desoxycholate agar (XLD) were evaluated using nonspiked chicken liver and pork sausage samples. The concentration of 1.2 g/L of pp3 in XLT4 gave the best overall results. In virtually all cases, the Salmonella colonies were larger and more black than on plain XLT4 without pp3. The improved XLT4 is recommended for more reliable detection of salmonellae from food, environmental and clinical samples.

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