Analysis of male sterility-related proteins of young panicle in rice (Oryza sativa L.) by two-dimensional electrophoresis

2005 ◽  
Vol 10 (3) ◽  
pp. 597-601 ◽  
Author(s):  
Hu Yao-jun ◽  
Wei Lei ◽  
Liu Shu-nan ◽  
Yu Jin-hong ◽  
Ding Yi
Keyword(s):  
Oryza Sativa ◽  
Male Sterility ◽  
Oryza Sativa L ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Young Panicle ◽  
Related Proteins ◽  
Dimensional Electrophoresis

scholarly journals Application of two-dimensional electrophoresis and mass spectrometry to screen endometriosis-related proteins

2014 ◽  
Vol 10 (1) ◽  
pp. 95-100
Author(s):  
HANIKEZI TUERXUN ◽  
YANMEI ZHANG ◽  
FEI JI ◽  
AIXINGZI AILI ◽  
XINHUA YANG ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Related Proteins ◽  
Dimensional Electrophoresis

Proteome analysis and identification of symbiosis-related proteins from Medicago truncatula Gaertn. by two-dimensional electrophoresis and mass spectrometry

2002 ◽  
Vol 23 (1) ◽  
pp. 122 ◽  
Author(s):  
Gwénaëlle Bestel-Corre ◽  
Eliane Dumas-Gaudot ◽  
Véréna Poinsot ◽  
Marc Dieu ◽  
Jean-François Dierick ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Medicago Truncatula ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Related Proteins ◽  
Dimensional Electrophoresis

scholarly journals GENETIC VARIATION IN PROTEINS: COMPARISON OF ONE-DIMENSIONAL AND TWO-DIMENSIONAL GEL ELECTROPHORESIS

Genetics ◽  
1983 ◽  
Vol 104 (2) ◽  
pp. 381-390
Author(s):  
Tracy McLellan ◽  
Giovanna Ferro-Luzzi Ames ◽  
Kishiko Nikaido
Keyword(s):  
Genetic Variation ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
One Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Related Proteins ◽  
Dimensional Electrophoresis ◽  
Denaturation Of Proteins ◽  
New Mutations ◽  
Limited Sensitivity

ABSTRACT Two proteins with known characteristics on one-dimensional gels were studied by two-dimensional electrophoresis to compare the sensitivities of the two methods in detecting genetic variation. Two-dimensional electrophoresis was found to be less sensitive than several types of one-dimensional gels in distinguishing variants of both proteins. Denaturation of proteins in urea in the two-dimensional method makes it possible to distinguish closely related proteins that differ from each other by units of charge. Many more types of variation in protein sequences can be distinguished on one-dimensional gels in the absence of denaturants. The estimates of heterozygosity based on two-dimensional gels are lower than those based on other methods, at least in part, because of the limited types of sequence differences that can be detected on two-dimensional gels. The application of two-dimensional electrophoresis to the measurement of genetic variation and to the detection of new mutations should be made carefully, in view of the limited sensitivity of the method in finding differences in sequence.

Detection of Cancer-related Proteins by Two-dimensional Electrophoresis

1984 ◽  
Vol 428 (1 Technology Im) ◽  
pp. 173-185 ◽  
Author(s):  
EGIL JELLUM ◽  
ANNE KARINE THORSRUD ◽  
MORTEN H. VATN ◽  
IVAR A. GRIMSTAD ◽  
IVAR BRENNHOVD ◽  
...  
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Related Proteins ◽  
Dimensional Electrophoresis

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

Improvement of Two-dimensional Electrophoresis of Rice (Oryza sativaL.) Proteomics

2012 ◽  
Vol 18 (5) ◽  
pp. 819 ◽  
Author(s):  
Yanhua YANG ◽  
Weitong CUI ◽  
Xiaoyong LIU ◽  
Keming ZHU ◽  
Keping CHEN
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
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