scholarly journals Cultivation ofMycobacterium leprae in artificial culture medium

1997 ◽  
Vol 12 (S1) ◽  
pp. 97-99
Author(s):  
S. K. Biswas
Keyword(s):  
Culture Medium ◽  
Artificial Culture ◽  
Artificial Culture Medium

scholarly journals A novel experimental approach for studying life-history traits of phytophagous arthropods utilizing an artificial culture medium

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kamila Karpicka-Ignatowska ◽  
Alicja Laska ◽  
Lechosław Kuczyński ◽  
Brian G. Rector ◽  
Mariusz Lewandowski ◽  
...  
Keyword(s):  
Life History ◽  
Host Plant ◽  
Life History Traits ◽  
Culture Medium ◽  
Interspecific Interactions ◽  
Suitable Condition ◽  
Mite Species ◽  
Artificial Culture ◽  
Wide Range ◽  
Artificial Culture Medium

AbstractExperimental approaches to studying life-history traits in minute herbivorous arthropods are hampered by the need to work with detached host plant material and the difficulty of maintaining that material in a suitable condition to support the animal throughout the duration of the test. In order to address this shortcoming, we developed a customizable agar-based medium modified from an established plant cell-culture medium to nourish detached leaves laid atop it while also preventing arthropods from escaping the experimental arena. The artificial culture medium was tested with two herbivorous mite species: the wheat curl mite (Aceria tosichella; Eriophyidae) and two-spotted spider mite (Tetranychus urticae; Tetranychidae). The proposed approach was a major improvement over a standard protocol for prolonged studies of individual eriophyid mites and also provided some benefits for experiments with spider mites. Moreover, the described method can be easily modified according to the requirements of host plant species and applied to a wide range of microherbivore species. Such applications include investigations of life-history traits and other ecological and evolutionary questions, e.g. mating or competitive behaviours or interspecific interactions, assessing invasiveness potential and predicting possible outbreaks. The approach presented here should have a significant impact on the advancement of evolutionary and ecological research on microscopic herbivores.

scholarly journals Design of an artificial culture medium to optimize Haslea ostrearia biomass and marennine production

2020 ◽  
Vol 45 ◽  
pp. 101653 ◽  
Author(s):  
R. Nghiem Xuan ◽  
I. Safitri ◽  
J.L. Mouget ◽  
J. Pruvost ◽  
V. Turpin ◽  
...  
Keyword(s):  
Culture Medium ◽  
Artificial Culture ◽  
Haslea Ostrearia ◽  
Artificial Culture Medium

scholarly journals Overproduction of Some Plant Growth Promoters by Rhizospheric Microorganisms on Natural Medium

2020 ◽  
pp. 7-21
Author(s):  
Shimaa E. Ibrahim ◽  
Heba Sh. Shehata ◽  
Hala F. Mohamed ◽  
Rawheya A. Salah El Din
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Aloe Vera ◽  
Growth Promoters ◽  
Artificial Culture ◽  
16S Rrna Analysis ◽  
Phosphorus Solubilization ◽  
Pseudomonas Monteilii ◽  
Synthetic Media ◽  
The Cost

Considering the nutritional values of Mentha viridis. L and Aloe veraplants, these plants can be utilized for the production of alternative cultivation media. The cost of artificial culture media is very high, and some components may be unavailable. Use of the plant-based culture media would drastically reduce the expense of the synthetic media. Fifteen bacterial isolates were isolated from Aloe vera rhizosphere, nine bacterial and nine actinomycetes isolates were isolated from Mentha viridis rhizosphere both cultivated in Sirs EL- Layan, El-Menoufia governorate, Egypt. In-vitroscreening was done for the production of indole acetic acid (IAA) and phosphorus solubilization. Results revealed that bacterial isolate No. MB4 produced a high amount of IAA(36.51 μg/ml) on the Mentha-based culture medium, No. A6 showed maximum IAA production (16.25μg/ml) on the Aloe vera-based culture medium and isolate No. MA6 was efficient in phosphorus solubilization (867.85μg/ml) that was isolated from the Mentha viridis. L rhizosphere.16s rRNA analysis of these isolates revealed they are (Pseudomonas monteilii strain CIP 104883, Streptomyces rochei strain DW3 and Kosakonia radicincitans strain DSM 16656 respectively.

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

Structural aspects of osmoregulation in porphyra

1978 ◽  
Vol 36 (2) ◽  
pp. 412-413
Author(s):  
C. Wiencke ◽  
A. Lauchli
Keyword(s):  
Culture Medium ◽  
Point Of View ◽  
Chemical Fixation ◽  
Cellular Organization ◽  
Osmotic Adaptation ◽  
Osmotic Balance ◽  
Activity Of Enzymes ◽  
Osmotic Agents ◽  
Vacuolar System ◽  
Structural Aspects

Osmoregulatory mechanisms in algae were investigated mainly from a physiological point of view (KAUSS 1977, HELLEBUST 1976). In Porphyra two osmotic agents, i. e. floridoside/isofloridoside (KAUSS 1968) and certain ions, such as K+ and Na+(EPPLEY et al. 1960) are considered for osmotic balance. Accumulations of ions (particularly Na+) in the cytoplasm during osmotic adaptation is improbable, because the activity of enzymes is generally inhibited by high ionic concentrations (FLOWERS et al. 1977).The cellular organization of Porphyra was studied with special emphasis on the development of the vacuolar system under different hyperosmotic conditions. Porphyra was cultivated at various strengths of the culture medium ASP 12 (PROVASOLI 1961) ranging from normal to 6 times concentrated (6x) culture medium. Por electron microscopy freeze fracturing was used (specimens fixed in 2% glutaraldehyde and incubated in 30% glycerol, preparation in a BALZERS BA 360 M apparatus), because chemical fixation gave poor results.

The significance of SEM in the diagnosis of haematopoietic malignancies

1978 ◽  
Vol 36 (2) ◽  
pp. 314-315
Author(s):  
Etienne de Harven ◽  
Nina Lampen
Keyword(s):  
Room Temperature ◽  
Culture Medium ◽  
Cell Attachment ◽  
Ethanol Dehydration ◽  
Moist Chamber ◽  
Efficient Alternative ◽  
Mononuclear Leucocytes ◽  
Fast Quenching ◽  
Freeze Dryer ◽  
Scanning Electron

Samples of heparinized blood, or bone marrow aspirates, or cell suspensions prepared from biopsied tissues (nodes, spleen, etc. ) are routinely prepared, after Ficoll-Hypaque concentration of the mononuclear leucocytes, for scanning electron microscopy. One drop of the cell suspension is placed in a moist chamber on a poly-l-lysine pretreated plastic coverslip (Mazia et al., J. Cell Biol. 66:198-199, 1975) and fifteen minutes allowed for cell attachment. Fixation, started in 2. 5% glutaraldehyde in culture medium at room temperature for 30 minutes, is continued in the same fixative at 4°C overnight or longer. Ethanol dehydration is immediately followed by drying at the critical point of CO2 or of Freon 13. An efficient alternative method for ethanol dehydrated cells is to dry the cells at low temperature (-75°C) under vacuum (10-2 Torr) for 30 minutes in an Edwards-Pearse freeze-dryer (de Harven et al., SEM/IITRI/1977, 519-524). This is preceded by fast quenching in supercooled ethanol (between -90 and -100°C).

Sign in / Sign up

Export Citation Format

Share Document