Studies on screening and isolation of α-amylase inhibitors of soil microorganisms (I)

1985 ◽  
Vol 8 (2) ◽  
pp. 67-75
Author(s):  
Jin Hwan Kwak ◽  
Eung Chil Choi ◽  
Byong Kak Kim
Keyword(s):  
Soil Microorganisms ◽  
Amylase Inhibitors

Soil response to chemicals used in a field experiment

2013 ◽  
Vol 27 (2) ◽  
pp. 151-158 ◽  
Author(s):  
S. Jezierska-Tys ◽  
A. Rutkowska
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Field Experiment ◽  
Soil Microorganisms ◽  
Block Design ◽  
Soil Samples ◽  
Alkaline Phosphatases ◽  
Soil Response ◽  
Negative Effect ◽  
Set Up

Abstract The effect of chemicals (Reglone 200 SL and Elastiq 550 EC) on soil microorganisms and their enzymatic activity was estimated. The study was conducted in a field experiment which was set up in the split-block design and comprised three treatments. Soil samples were taken six times, twice in each year of study. The results showed that the application of chemicals generally had no negative effect on the number of soil microorganisms. The application of Reglone 200 SL caused an increase of proteolytic and ureolytic activity and affected the activity of dehydrogenases, acid and alkaline phosphatases in the soil. The soil subjected of Elastiq 550 EC was characterized by lower activity of dehydrogenases, protease, urease and alkaline phosphatase.

ISOLATION of a AMYLASE INHIBITORS from MUNGBEAN and SOYBEAN and INHIBITORY EFFECT on HUMAN SALIVARY and PORCINE PANCREATIC AMYLASE

2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Budiasih Wahyuntari ◽  
Martinus Nicoadi Tekol Tekol
Keyword(s):  
Inhibitory Effect ◽  
Pancreatic Amylase ◽  
Sds Page ◽  
Amylase Inhibitors

Penghambat alfa amilase adalah salah satu komponen dalam suplemen makanan yang telah lama digunakan untuk terapi kegemukan karena penghambat amilase mempengaruhi metabolisme karbohidrat dalam sistem pencernaan. Sejumlah peneliti melaporkan terdapat dua grup penghambat amilase, yaitu protein dan non protein. Penghambat protein dilaporkan terdapat dalam kelompok kacang-kacangan dan biji-bijian. Tujuan penelitian ini adalah untuk mengisolasi penghambat protein yang terdapat dalam kacang hijau dan kedele. Kacang hijau dan kedele merupakan kacang-kacangan yang penting dalam makanan popular di Indonesia. Penghambat protein diendapkan dengan konsentrasi bertingkat garam ammonium sulfat [(NH)4SO4] dari 30-70%. Penghambat protein diuji terhadap amilase saliva manusia (HSA) danamilase pankreas babi (PPA), serta kestabilannya terhadap pemanasan pada 100oC selama 30 menit. Hasil penelitian menunjukkan bahwa semua endapan jenuh dari semua konsentrasi amonium sulfat yang diuji menghambat PPA, tetapi tidak semua endapan jenuh tersebut dapatmenghambat HSA. Hanya semua endapan jenuh (NH)4SO4 dari kacang hijau yang dapat menghambat HSA, dan penghambatan tertinggi terhadap HSA adalah endapan jenuh (NH)4SO4 50%. Endapan jenuh (NH)4SO4 40 % dari kedele putih dan endapan jenuh (NH)4SO4 60% dari kedele hitam dengan masing-masing penghambatan 98.67; 26.86 and27.63%. Endapan jenuh (NH)4SO4 60-70% dari kacang hijau, 50% dari kedele putih dan 50% dari kedele hitam menghambat PPA 100%. Pemanasan penghambat pada 100oC selama 30 menit hampir tidak mempengaruhi penghambatannya terhadap PPA. Profil protein juga diamati menggunakan analisis SDS/PAGE.

Problems in the Use of Agar for the Enumeration of Soil Microorganisms

1960 ◽  
Vol 24 (1) ◽  
pp. 61-62 ◽  
Author(s):  
K. C. Marshall ◽  
Jean S. Whiteside ◽  
M. Alexander
Keyword(s):  
Soil Microorganisms

Screening of Actinomycetes For α-amylase Inhibitors Production

2019 ◽  
Vol 15 (1) ◽  
pp. 41-45
Author(s):  
Shivabai Chandwad ◽  
Sudhakar Gutte
Keyword(s):  
Inhibitory Activity ◽  
Enzyme Inhibitors ◽  
Spectroscopic Method ◽  
Solid Material ◽  
Soil Samples ◽  
Assay Method ◽  
Novel Drugs ◽  
Pre Treatment ◽  
Diabetic Treatment ◽  
Amylase Inhibitors

Background:Diabetes mellitus is the most common and fastest growing disease in the world. One of the therapies to treat diabetes is the inhibition of α-amylase activity by inhibitors from microbial and plant source. Actinomycetes are potential sources of enzyme inhibitors, drugs, amino acids, vitamins etc.Objective:Our work mainly highlights the isolation of actinomycetes from soil samples of different habitats and screening of α -amylase inhibitors.Methods:Actinomycetes were isolated from soil samples of different habitats by different methods; these include a variety of pre-treatment of soil samples in combination with an appropriate supplement medium with selective antibacterial agents. Isolated actinomycetes grown in fermentation condition and metabolites were extracted with Isopropyl alcohol and concentrated to obtain solid material. The extract of each isolate was tested for α -amylase inhibition using starch Iodine plate method and DNS- spectroscopic method.Results:Total 110 actinomycetes strains were isolated from various sources. Among 110 extracts of actinomycetes, eight extracts have shown positive results for α-amylase inhibition in starch Iodine plate assay method. Extracts selected from primary results were used for the confirmation of inhibitory activity using DNS- spectroscopic method. Out of eight extracts, six extracts showed Porcine pancreatic α -amylase inhibitory activity ranging from 40-86%. The actinomycetes strains that produce α -amylase inhibitory activity are A-24, A-29, B-5, B-18, C-15 and D-24.Conclusion:These results show that actinomycetes are a potential source for α -amylase inhibitors, which may lead to valuable novel drugs for diabetic treatment.

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