Ethanol production from spent sulfite liquor fortified by hydrolysis of pulp mill primary clarifier sludge

Abstract Eucalyptus residues from pulp mill were pretreated with aqueous ammonia soaking (AAS) method to improve the efficiency of enzymatic hydrolysis. The optimized condition of AAS was obtained by response surface methodology. Meanwhile, hydrogen peroxide was introduced into the AAS system to modify the AAS pretreatment (AASP). The results showed that a fermentable sugar yield of 64.96 % was obtained when the eucalypt fibers were pretreated at the optimal conditions, with 80 % of ammonia (w/w) for 11 h and keeping the temperature at 90 °C. In further research it was found that the addition of H2O2 to the AAS could improve the pretreatment efficiency. The delignification rate and enzymatic digestibility were increased to 64.49 % and 73.85 %, respectively, with 5 % of hydrogen peroxide being used. FTIR analysis indicated that most syringyl and guaiacyl lignin and a trace amount of xylan were degraded and dissolved during the AAS and AASP pretreatments. The CrI of the raw material was increased after AAS and AASP pretreatments, which was attributed to the removal of amorphous portion. SEM images showed that microfibers were separated and explored from the initial fiber structure after AAS pretreatment, and the AASP method could improve the destructiveness of the fiber surface.

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Prospects for the utilization of the endogenous enzymes in sorghum malt in the hydrolysis of starch: Case study with utilization of breadfruit starch for ethanol production

Food Biotechnology ◽

10.1080/08905439409549878 ◽

1994 ◽

Vol 8 (2-3) ◽

pp. 243-255 ◽

Cited By ~ 5

Author(s):

B. O. Solomon ◽

S. K. Layokun ◽

A. O. Idowu ◽

M. O. Ilori

Keyword(s):

Ethanol Production ◽

Sorghum Malt ◽

Hydrolysis Of ◽

Endogenous Enzymes

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Process integration study of a kraft pulp mill converted to an ethanol production plant – Part A: Potential for heat integration of thermal separation units

Applied Thermal Engineering ◽

10.1016/j.applthermaleng.2011.10.010 ◽

2012 ◽

Vol 35 ◽

pp. 81-90 ◽

Cited By ~ 20

Author(s):

Rickard Fornell ◽

Thore Berntsson

Keyword(s):

Ethanol Production ◽

Kraft Pulp ◽

Process Integration ◽

Pulp Mill ◽

Plant Part ◽

Heat Integration ◽

Production Plant ◽

Kraft Pulp Mill

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Selection and identification of xylose-fermenting yeast strains for ethanol production from lignocellulosic biomass

Boletim do Centro de Pesquisa de Processamento de Alimentos ◽

10.5380/bceppa.v36i1.59557 ◽

2019 ◽

Vol 36 (1) ◽

Author(s):

Juliana Alves Araújo ◽

Thiago Lucas de Abreu-Lima ◽

Solange Cristina Carreiro

Keyword(s):

Ethanol Production ◽

Lignocellulosic Biomass ◽

Fossil Fuels ◽

Reference Strain ◽

Molecular Techniques ◽

Replica Plating ◽

Fermentative Capacity ◽

Edible Crops ◽

Plant Exudates ◽

Hydrolysis Of

Ethanol production from lignocellulosic biomass is of economic interest due to the pressure to reduce fossil fuels consumption and land use for non-edible crops. Xylose is one of the main sugars obtained by hydrolysis of hemicellulose fraction of biomass, but industrial yeasts cannot ferment it. This work aimed to select, characterize and identify xylose-fermenting yeasts from Brazilian microorganisms collections with potential use in ethanol production. Xylose assimilation was tested by replica plating, and fermentation was tested with Durham tubes. Xylose-fermenting strains had their fermentative capacity quantified and compared to a reference strain (Scheffersomyces stipitis UFMG-IMH 43.2) and were identified by molecular techniques. Three strains isolated from plant exudates were able to ferment xylose and showed fermentative parameters similar to the reference strain. Two strains were identified as Candida parapsilosis and one was identified as Meyerozyma guilliermondii. The findings show the potential biotechnological use of these microorganisms.

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