Effects on GSH synthesis in Chinese cabbage when the culturing solution is supplemented with ammonium sulfate or the constituent amino acids for glutathione

2005 ◽  
Vol 48 (4) ◽  
pp. 404-410 ◽  
Author(s):  
Ju-Sung Kim ◽  
Sang-Gyu Seo ◽  
Sun-Hyung Kim ◽  
Kenji Usui ◽  
Ie-Sung Shim
Keyword(s):  
Amino Acids ◽  
Ammonium Sulfate ◽  
Chinese Cabbage

The effect of ammonium sulfate on the solubility of amino acids in water at (298.15 and 323.15)K

2009 ◽  
Vol 41 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Luísa A. Ferreira ◽  
Eugénia A. Macedo ◽  
Simão P. Pinho
Keyword(s):  
Amino Acids ◽  
Ammonium Sulfate

INFLUENCE OF NUTRIENTS ON PERITHECIAL PRODUCTION OF VENTURIA INAEQUALIS (CKE.) WINT.

1965 ◽  
Vol 43 (8) ◽  
pp. 959-965 ◽  
Author(s):  
R. G. Ross ◽  
S. A. Hamlin
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ammonium Sulfate ◽  
Narrow Range ◽  
Venturia Inaequalis ◽  
Synthetic Medium ◽  
Amino Acid Nitrogen ◽  
Low Concentrations ◽  
Nitrogen Concentrations ◽  
Ascospore Formation

Nitrates, urea, and amino acids were suitable sources of nitrogen, within a narrow range of concentrations, for perithecial development of Venturia inaequalis in a synthetic medium. Ammonium sulfate was not. The concentration of amino acid nitrogen required to inhibit the formation of perithecia varied with the amino acid. Zinc appeared to stimulate perithecial production. Amino acids differed in the nitrogen concentrations required to inhibit formation of perithecia. Perithecia developed in low concentrations of arabinose, glucose, sucrose, lactose, and maltose but did not develop in xylose, ribose, fructose, mannose, or galactose. The disaccharides were particularly suitable. Thiamine appeared to be necessary for ascospore formation.

Effects of amino acids on Thiobacillus acidophilus. II. Threonine deaminase

1980 ◽  
Vol 26 (3) ◽  
pp. 385-388 ◽  
Author(s):  
Gérald Proteau ◽  
Marvin Silver
Keyword(s):  
Amino Acids ◽  
Enzyme Activity ◽  
Ammonium Sulfate ◽  
Active Site ◽  
Binding Sites ◽  
Threonine Deaminase ◽  
Low Concentrations ◽  
High Concentrations ◽  
Thiobacillus Acidophilus ◽  
Optimal Ph

Biosynthetic L-threonine deaminase was partially purified 73-fold with a 60% recovery from Thiobacillus acidophilus by ammonium sulfate fractionation and by Sepharose 6B-C1 chromatography. The optimal pH for enzyme activity was between 9.0 and 10.0 and no optimal pH shift was observed in the presence of L-isoleucine, an inhibitor. The enzyme was effectively inhibited by L-isoleucine and showed homotropic interaction only in the presence of L-isoleucine.Kinetic studies indicate that there are at least two threonine binding sites and at least two isoleucine binding sites. The Km for threonine is 2.5 × 10−3 M. The inhibition due to isoleucine is reversed by low concentrations of L-valine. L-Valine at high concentrations acts as a substrate analogue and competitively inhibits L-threonine binding at the active site; the K1 is 1.6 × 10−2 M.

scholarly journals An Extracellular Protease of Streptococcus gordonii Hydrolyzes Type IV Collagen and Collagen Analogues

1999 ◽  
Vol 67 (1) ◽  
pp. 271-278 ◽  
Author(s):  
Zaira E. Juarez ◽  
Murray W. Stinson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ammonium Sulfate ◽  
Type Iv Collagen ◽  
Bacterial Endocarditis ◽  
Acid Mixture ◽  
Enzyme Release ◽  
Streptococcus Gordonii ◽  
Type Iv Collagenase ◽  
Type Iv

ABSTRACT Streptococcus gordonii is a frequent cause of infective bacterial endocarditis, but its mechanisms of virulence are not well defined. In this study, streptococcal proteases were recovered from spent chemically defined medium (CDM) and fractionated by ammonium sulfate precipitation and by ion-exchange and gel filtration column chromatography. Three proteases were distinguished by their different solubilities in ammonium sulfate and their specificities for synthetic peptides. One of the enzymes cleaved collagen analogs Gly-Pro 4-methoxy-β-naphthylamide, 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA), and p-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg (pZ-peptide) and was released from the streptococci while complexed to peptidoglycan fragments. Treatment of this protease with mutanolysin reduced its 180- to 200-kDa mass to 98 kDa without loss of enzymatic activity. The purified protease cleaved bovine gelatin, human placental type IV collagen, and the Aα chain of fibrinogen but not albumin, fibronectin, laminin, or myosin. Enzyme activity was inhibited by phenylmethylsulfonyl fluoride, indicating that it is a serine-type protease. Maximum production of the 98-kDa protease occurred during growth of S. gordonii CH1 in CDM containing 0.075% total amino acids at pH 7.0 with minimal aeration. Higher initial concentrations of amino acids prevented the release of the protease without reducing cell-associated enzyme levels, and the addition of an amino acid mixture to an actively secreting culture stopped further enzyme release. The purified protease was stored frozen at −20°C for several months or heated at 50°C for 10 min without loss of activity. These data indicate that S. gordonii produces an extracellular gelatinase/type IV collagenase during growth in medium containing minimal concentrations of free amino acids. Thus, the extracellular enzyme is a potential virulence factor in the amino acid-stringent, thrombotic, valvular lesions of bacterial endocarditis.

TRANSAMINATION IN PLANTS: THE SPECIFICITY OF AN AMINOTRANSFERASE FROM MUNG BEAN

1965 ◽  
Vol 43 (6) ◽  
pp. 723-730 ◽  
Author(s):  
Oluf L. Gamborg
Keyword(s):  
Amino Acids ◽  
Ammonium Sulfate ◽  
Mung Bean ◽  
Deae Cellulose ◽  
Phaseolus Aureus ◽  
Ammonium Sulfate Precipitation ◽  
Competitive Inhibitors

A study has been made of the specificity of an aminotransferase from mung bean (Phaseolus aureus Roxb.). The enzyme was purified 40- to 60-fold by using Sephadex G-50, ammonium sulfate precipitation, DEAE-cellulose, and hydroxylapatite. In the presence of pyruvate the enzyme transaminated a number of cyclic and aliphatic amino acids. Some of the better substrates were lysine, arginine, ornithine, glutamine, methionine, leucine, 4-fiuorophenyl-alanine, phenylalanine, tyrosine, tryptophan, 3,4-dihydroxyphenylalanine, and γ-phenylbutyrine. Threonine, serine, and glycine were not transaminated. Lysine, methionine, and glutamate were competitive inhibitors of the transamination of phenylalanine.

Hygroscopicity of amino acids and their effect on the water uptake of ammonium sulfate in the mixed aerosol particles

2020 ◽  
Vol 734 ◽  
pp. 139318
Author(s):  
Qingwei Luo ◽  
Juan Hong ◽  
Hanbing Xu ◽  
Shuang Han ◽  
Haobo Tan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ammonium Sulfate ◽  
Water Uptake ◽  
Aerosol Particles

Impacts of Nitrogen (Urea) Fertilization on Cadmium Accumulation Nitrate Contents and Nutritive Values in Chinese Cabbage

2011 ◽  
Vol 393-395 ◽  
pp. 809-817
Author(s):  
Li Zhao ◽  
Fei Juan Wang ◽  
Hui Yang ◽  
Yu Xing Duan ◽  
Chen Zhu
Keyword(s):  
Amino Acids ◽  
Food Security ◽  
Chinese Cabbage ◽  
Nitrogen Fertilization ◽  
Soluble Sugar ◽  
Water Soluble ◽  
Natural Soil ◽  
Edible Part ◽  
Nutritive Values ◽  
Urea Fertilization

Pot-mode cultivation experiments were carried out to study the impacts of nitrogen（urea）fertilization in different level on Cadmium (Cd) accumulation, nitrate contents and nutritive values in Chinese cabbage, The results showed that: at different level of Cd artificial enriched soil, with applied urea increased, the accumulation of Cd in edible part of Chinese cabbage gradually decreased. Nitrate Contents in edible part have a positive correlation with the concentration of Cd in soil and the nitrogen fertilizer applied. In the natural soil (with contaminated Cd in concentration of 0.18 mg/kg), when the urea was fertilized by 255 kg/hm2 to 330 kg/hm2, Cd and nitrate contents of edible parts of cabbage were within the national food security limits, which could guarantee the food security of Chinese cabbage. Increasing nitrogen fertilization in the form of urea has a certain effect on amino acids contents but little effect on soluble sugar contents in Chinese cabbage, when the concentration of urea was 320 kg/hm2, amino acids contents of cabbage reached the highest level of 355 mg/kg1. Increasing nitrogen fertilization could lower down the contents of water-soluble Cd and the available Cd in soil, thereby reduce the absorption of Cd by Chinese cabbage. To a certain extent, increasing nitrogen fertilization could increase the food security of Chinese cabbage.

scholarly journals SILICA SLAG AND BARK SLAG INFLUENCE INFECTION OF PLASMODIOPHORA BRASSICA INFECTIONIN AN INTENSIVE CHINESE CABBAGE PRODUCTION SYSTEM.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 607c-607
Author(s):  
Loong-sheng Chang ◽  
Chun-yen Yeh ◽  
Chien-hwa Liao
Keyword(s):  
High Altitude ◽  
Ammonium Sulfate ◽  
Chinese Cabbage ◽  
Root Hair ◽  
Production System ◽  
Vegetable Production ◽  
Slaked Lime ◽  
Race 1 ◽  
Northwestern Coast ◽  
Calcium Superphosphate

Race 1 of Plasmodiophora brassica isolated from high altitude of vegetable production district induced clubroot on cabbage, and Chinese cabbage. Inoculation of race from northwestern coast of Taiwan resulted clubroot of Chinese cabbage neither in cabbage. The addition of bark slag or silica slag significantly decreased clubroot infection and increased the weight of Chinese cabbage in the infected field. The addition of 3 gram slaked lime +1 gram KC1 +1.78 gram ammonium sulfate + 1 gram calcium superphosphate at 500 gram soil 2 month after transplanting increased dry wight of cabbage and decreased infection root hair followed by inoculation of race 1.

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