Production of nitric oxide under Ultraviolet-B irradiation is mediated by hydrogen peroxide through activation of nitric oxide synthase

2008 ◽  
Vol 51 (6) ◽  
pp. 395-400 ◽  
Author(s):  
Lingang Zhang ◽  
Liqun Zhao
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Nitric Oxide Synthase ◽  
Ultraviolet B ◽  
Ultraviolet B Irradiation ◽  
Oxide Synthase

Change of Inducible Nitric Oxide Synthase Expression by Ultraviolet B Irradiation on the Skin of a Rat

2001 ◽  
Vol 13 (1) ◽  
pp. 16
Author(s):  
Hyoung Chul Yoo ◽  
Hyong-Woo Lee ◽  
Jeong Eun Lee ◽  
Jee Bum Lee ◽  
Young Ho Won ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Ultraviolet B ◽  
Ultraviolet B Irradiation ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein fromBacillus cereus

2010 ◽  
Vol 2010 ◽  
pp. 1-4 ◽  
Author(s):  
Heather J. Montgomery ◽  
Andrea L. Dupont ◽  
Hilary E. Leivo ◽  
J. Guy Guillemette
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Nitric Oxide Synthase ◽  
Expression And Purification ◽  
Spectral Shifts ◽  
Reconstituted System ◽  
Low Levels ◽  
And Function ◽  
Oxide Synthase ◽  
Reductase Domain

The nitric oxide synthase-like protein fromBacillus cereus(bcNOS) has been cloned, expressed, and characterized. This small hemeprotein (356 amino acids in length) has a mass of 43 kDa and forms a dimer. The recombinant protein showed similar spectral shifts to the mammalian NOS proteins and could bind the substrates L-arginine andNG-hydroxy-L-arginine as well as the ligand imidazole. Low levels of activity were recorded for the hydrogen peroxide-dependent oxidation ofNG-hydroxy-L-arginine and L-arginine by bcNOS, while a reconstituted system with the rat neuronal NOS reductase domain showed no activity. The recombinant bcNOS protein adds to the complement of bacterial NOS-like proteins that are used for the investigation of the mechanism and function of NO in microorganisms.

Hydrogen Peroxide-Supported Oxidation of NG-Hydroxy-L-Arginine by Nitric Oxide Synthase

Biochemistry ◽  
1995 ◽  
Vol 34 (6) ◽  
pp. 1930-1941 ◽  
Author(s):  
Robert A. Pufahl ◽  
John S. Wishnok ◽  
Michael A. Marletta
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Nitric Oxide Synthase ◽  
Oxide Synthase
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