Effects of GM1-ganglioside and α-sialyl cholesterol on amino acid uptake, protein synthesis, and Na+, K+-ATPase activity in superior cervical and nodose ganglia excised from adult rats

1990 ◽  
Vol 13 (1-2) ◽  
pp. 33-46 ◽  
Author(s):  
Masato Ando ◽  
Yuhzo Nakashima ◽  
Yutaka Nagata
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Atpase Activity ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
Gm1 Ganglioside ◽  
Adult Rats ◽  
Nodose Ganglia

Effect of Exogenous Gangliosides on Amino Acid Uptake and Na+,K+-ATPase Activity in Superior Cervical and Nodose Ganglia of Rats

1987 ◽  
Vol 49 (1) ◽  
pp. 201-207 ◽  
Author(s):  
Yutaka Nagata ◽  
Masato Ando ◽  
Mitsuyoshi Iwata ◽  
Atsushi Hara ◽  
Tamotsu Taketomi
Keyword(s):  
Amino Acid ◽  
Atpase Activity ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
Nodose Ganglia

scholarly journals KINETIC STUDIES ON AMINO ACID UPTAKE AND PROTEIN SYNTHESIS IN LIVER OF TEMPERATURE ACCLIMATED TOADFISH

1973 ◽  
Vol 145 (3) ◽  
pp. 472-481 ◽  
Author(s):  
AUDREY E. V. HASCHEMEYER ◽  
ROGER PERSELL
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Kinetic Studies ◽  
Amino Acid Uptake ◽  
Acid Uptake

scholarly journals NATURE OF THE INHIBITION OF PROTEIN SYNTHESIS BY ETHIONINE AND AMINO ACID UPTAKE IN EARLY SEA URCHIN EMBRYOS

1977 ◽  
Vol 19 (1) ◽  
pp. 31-39 ◽  
Author(s):  
YOSHITAKE MANO ◽  
NORIO SUZUKI ◽  
HIROYUKI SHIMATAKE
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Sea Urchin ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
Sea Urchin Embryos ◽  
Inhibition Of Protein Synthesis

scholarly journals Action of growth hormone in vitro on the net uptake and incorporation into protein of amino acids in muscle from intact rabbits given protein-deficient diets

1976 ◽  
Vol 35 (1) ◽  
pp. 1-10 ◽  
Author(s):  
M. R. Turner ◽  
P. J. Reeds ◽  
K. A. Munday
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Protein Synthesis ◽  
Amino Acid ◽  
De Novo ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

1. Net amino acid uptake, and incorporation into protein have been measured in vitro in the presence and absence of porcine growth hormone (GH) in muscle from intact rabbits fed for 5 d on low-protein (LP), protein-free (PF) or control diets.2. In muscle from control and LP animals GH had no effect on the net amino acid uptake but stimulated amino acid incorporation into protein, although this response was less in LP animals than in control animals.3. In muscle from PF animals, GH stimulated both amino acid incorporation into protein and the net amino acid uptake, a type of response which also occurs in hypophysectomized animals. The magnitude of the effect of GH on the incorporation of amino acids into protein was reduced in muscle from PF animals.4. The effect of GH on the net amino acid uptake in PF animals was completely blocked by cycloheximide; the uptake effect of GH in these animals was dependent therefore on de novo protein synthesis.5. It is proposed that in the adult the role of growth hormone in protein metabolism is to sustain cellular protein synthesis when there is a decrease in the level of substrate amino acids, similar to that which occurs during a short-term fast or when the dietary protein intake is inadequate.

scholarly journals Action of insulin and growth hormone on protein synthesis in muscle from non-hypophysectomized rabbits

1971 ◽  
Vol 125 (2) ◽  
pp. 515-520 ◽  
Author(s):  
P. J. Reeds ◽  
K. A. Munday ◽  
M. R. Turner
Keyword(s):  
Amino Acids ◽  
Growth Hormone ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Incubation Medium ◽  
Amino Acid Uptake ◽  
Diaphragm Muscle ◽  
Acid Uptake

The separate effects of insulin and growth hormone on the uptake and incorporation of five amino acids into diaphragm muscle from non-hypophysectomized rabbits has been examined. Both growth hormone and insulin, when present in the medium separately, stimulated the incorporation into protein of the amino acids, leucine, arginine, valine, lysine and histidine. Insulin also stimulated amino acid uptake, but growth hormone did not. When insulin and growth hormone were present in the incubation medium together, the uptake and incorporation of valine, the only amino acid studied under these conditions, tended to be greater than the sum of the separate effects of the two hormones.

Amino acid uptake regulation by cell growth in cultured hepatocytes isolated from fetal and adult rats

1992 ◽  
Vol 12 (2) ◽  
pp. 135-141 ◽  
Author(s):  
S. Leoni ◽  
S. Spagnuolo ◽  
M. Massimi ◽  
F. Terenzi ◽  
L. Conti Devirgiliis
Keyword(s):  
Amino Acid ◽  
Cell Growth ◽  
Growth Inhibition ◽  
Cell Density ◽  
Amino Acid Uptake ◽  
Growth Stimulation ◽  
Acid Uptake ◽  
Adult Rats ◽  
Dependent Growth ◽  
Differentiation Stage

Amino acid uptake mediated by system A was studied in cultured fetal and adult hepatocytes, subjected to growth stimulation by EGF and insulin, or to growth inhibition by high cell density. The mitogenic stimulation induced a strong transport increase only in fetal cells, while the cell density-dependent growth inhibition, probably mediated by molecules present on adult hepatocyte membranes, provoked the decrease of amino acid uptake only in the adult cells. The results indicate that the different modulation of amino acid transport by cell growth is dependent on the age and the differentiation stage of hepatocytes.

Protein and energy metabolism in the pre-implantation cattle embryo

2001 ◽  
Vol 26 (2) ◽  
pp. 443-446 ◽  
Author(s):  
D.G. Morris ◽  
P. Humpherson ◽  
H.J. Leese ◽  
J.M. Sreenan
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Energy Metabolism ◽  
Metabolic Rate ◽  
Protein Content ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
Glucose Utilisation ◽  
Embryonic Loss

AbstractThere is no information on the metabolism of the cattle embryo during the period from day 8 to 16 a period of greatest embryonic loss. In this study the rate of protein synthesis and phosphorylation was measured in 13 to 15 day old cattle embryos. The rate of glucose utilisation and amino acid uptake/efflux by day 14 to 16 embryos was also measured. Protein synthesis and phosphorylation activity when expressed per unit of protein decreased with increasing embryo size and age. Similarly the rate of glucose utilisation was greatest for the earlier day 14 embryos. Embryos differed in their requirement for different amino acids. The pattern of uptake/efflux was similar to that of the earlier day 7 embryo. This study suggests that the metabolic rate of cattle embryos expressed per unit of protein content tends to decrease with increasing age and size from the initial burst of activity at day 13 around the time that expansion of the embryo begins.

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