Thrombolytic effect of Siddha herbal formulation Sikkanjar Manapagu using in-Silico model

Background: Thromboembolic disorders are one of the important causes leading to death. In the Siddha system of medicine, many drugs have been mentioned in the literature and their thrombolytic potential needs to be scientifically evaluated. Aim: The study aims to perform the In Silico computational studies of Phytoconstituents of Siddha formulation Sikkanjar Manapagu (SM) to evaluate its thrombolytic potential. Methods: Autodock program was used for the molecular docking studies of the retrieved phytoconstituents such as Zingiberene, Gingerenone-A, 6 Gingerol of Zingiber officinale, Menthol, Luteolin, Citronellol of Mentha arvensis, Eugenol, Limonene, Myrcene, and Linalool of Citrus aurantium against target protein Human Plasminogen Activation Loop Peptide - PDB 4DCB. Results: A total of ten compounds were screened, of these Zingiberene, Menthol, Citronellol, Eugenol, Limonene, Myrcene, and Linalool showed high binding against active amino acid residue 195. Conclusion: Based on further experiments and clinical trials, the formulation Sikkanjar Manapagu could be proved to be effective in thrombolytic treatment.

Glutamic acid as green and bio-based α-amino acid catalyst promoted one-pot access to polyfunctionalized dihydro-2-oxypyrroles

A highly versatile and convenient synthetic route for biologically active ?-amino acid, glutamic acid catalyzed facile and mild preparation of polyfunctionalized dihydro-2-oxypyrroles via one-pot, four condensation domino reaction between aromatic/aliphatic amines, dialkyl acetylenedicarboxylates and formaldehyde have been studied. The route includes green, biodegradable and inexpensive ?-amino acid catalyst, high atom-economy, simplicity of operation and work-up procedures, without chromatographic purification steps. The solid catalyst, non-toxic or hazardous, easily handled with mild reaction conditions and excellent yields are the notable benefits of the highly efficient and expedient synthesis of these products.