Construction of chimeric cytosolic fructose-1,6-bisphosphatases by insertion of a chloroplastic redox regulatory cluster

2004 ◽  
Vol 60 (1) ◽  
pp. 7-21 ◽  
Author(s):  
R. Cazalis ◽  
A. Chueca ◽  
M. Sahrawy ◽  
J. López-Gorgé
Keyword(s):  
Regulatory Cluster

Regulatory Cluster Analysis

1995 ◽  
pp. 21-29
Author(s):  
Dan Craigen ◽  
Susan Gerhart ◽  
Ted Ralston
Keyword(s):  
Cluster Analysis ◽  
Regulatory Cluster

Ubr1-induced selective endo-phagy/autophagy protects against the endosomal and Ca2+-induced proteostasis disease stress

2021 ◽  
Author(s):  
Ben B Wang ◽  
Haijin Xu ◽  
Sandra Isenmann ◽  
Cheng Huang ◽  
Xabier Elorza-Vidal ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
Protein Quality ◽  
Brain Disorders ◽  
Lysosomal Degradation ◽  
Defence Mechanisms ◽  
Selective Autophagy ◽  
Escrt Machinery ◽  
Endosomal Compartment ◽  
Regulatory Cluster

The defence mechanisms against endo-lysosomal homeostasis stress remain incompletely understood. Here, we identify Ubr1 as a protein quality control (QC) ubiquitin ligase that counteracts proteostasis stress by enhancing cargo selective autophagy for lysosomal degradation. Astrocyte regulatory cluster membrane protein MLC1 mutations increased intracellular Ca2+ and caused endosomal compartment stress by fusion and enlargement. Endosomal protein QC pathway using ubiquitin QC ligases CHIP and Ubr1 with ESCRT-machinery was able to target only a fraction of MLC1-mutants for lysosomal degradation. As a consequence of the endosomal stress, we found an alternative QC route dependent on Ubr1, SQSTM1/p62 and arginylation to bypass MLC1-mutants to endosomal autophagy (endo-phagy). Significantly, this unfolded a general biological endo-lysosomal QC pathway for arginylated Ubr1-SQSTM1/p62 autophagy targets during Ca2+-assault. Conversely, the loss of Ubr1 with the absence of arginylation elicited endosomal compartment stress. These findings underscore the critical housekeeping role of Ubr1-dependent endo-phagy/autophagy in constitutive and provoked endo-lysosomal proteostasis stress, and link Ubr1 to Ca2+-homeostasis and proteins implicated in various diseases including cancers and brain disorders.

scholarly journals Elucidation of the hrp Clusters of Xanthomonas oryzae pv. oryzicola That Control the Hypersensitive Response in Nonhost Tobacco and Pathogenicity in Susceptible Host Rice

2006 ◽  
Vol 72 (9) ◽  
pp. 6212-6224 ◽  
Author(s):  
Li-fang Zou ◽  
Xing-ping Wang ◽  
Yong Xiang ◽  
Bing Zhang ◽  
Yu-Rong Li ◽  
...  
Keyword(s):  
Hypersensitive Response ◽  
Inducible Promoter ◽  
Xanthomonas Oryzae ◽  
Complementation Test ◽  
Bacterial Leaf Streak ◽  
Rice Seedlings ◽  
Susceptible Host ◽  
The Core ◽  
Cluster A ◽  
Regulatory Cluster

ABSTRACT Xanthomonas oryzae pv. oryzicola, the cause of bacterial leaf streak in rice, possesses clusters of hrp genes that determine its ability to elicit a hypersensitive response (HR) in nonhost tobacco and pathogenicity in host rice. A 27-kb region of the genome of X. oryzae pv. oryzicola (RS105) was identified and sequenced, revealing 10 hrp, 9 hrc (hrp conserved), and 8 hpa (hrp-associated) genes and 7 regulatory plant-inducible promoter boxes. While the region from hpa2 to hpaB and the hrpF operon resembled the corresponding genes of other xanthomonads, the hpaB-hrpF region incorporated an hrpE3 gene that was not present in X. oryzae pv. oryzae. We found that an hrpF mutant had lost the ability to elicit the HR in tobacco and pathogenicity in adult rice plants but still caused water-soaking symptoms in rice seedlings and that Hpa1 is an HR elicitor in nonhost tobacco whose expression is controlled by an hrp regulator, HrpX. Using an Hrp phenotype complementation test, we identified a small hrp cluster containing the hrpG and hrpX regulatory genes, which is separated from the core hrp cluster. In addition, we identified a gene, prhA (plant-regulated hrp), that played a key role in the Hrp phenotype of X. oryzae pv. oryzicola but was neither in the core hrp cluster nor in the hrp regulatory cluster. A prhA mutant failed to reduce the HR in tobacco and pathogenicity in rice but caused water-soaking symptoms in rice. This is the first report that X. oryzae pv. oryzicola possesses three separate DNA regions for HR induction in nonhost tobacco and pathogenicity in host rice, which will provide a fundamental base to understand pathogenicity determinants of X. oryzae pv. oryzicola compared with those of X. oryzae pv. oryzae.

scholarly journals An enhancer cluster controls gene activity and topology of the SCN5A-SCN10A locus in vivo

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Joyce C. K. Man ◽  
Rajiv A. Mohan ◽  
Malou van den Boogaard ◽  
Catharina R. E. Hilvering ◽  
Catherine Jenkins ◽  
...  
Keyword(s):  
Mouse Genome ◽  
Genomic Region ◽  
Cardiac Conduction ◽  
Gene Promoters ◽  
And Topology ◽  
Super Enhancer ◽  
Cardiac Sodium Channel ◽  
Regulatory Cluster ◽  
Normal Embryonic Development

Abstract Mutations and variations in and around SCN5A, encoding the major cardiac sodium channel, influence impulse conduction and are associated with a broad spectrum of arrhythmia disorders. Here, we identify an evolutionary conserved regulatory cluster with super enhancer characteristics downstream of SCN5A, which drives localized cardiac expression and contains conduction velocity-associated variants. We use genome editing to create a series of deletions in the mouse genome and show that the enhancer cluster controls the conformation of a >0.5 Mb genomic region harboring multiple interacting gene promoters and enhancers. We find that this cluster and its individual components are selectively required for cardiac Scn5a expression, normal cardiac conduction and normal embryonic development. Our studies reveal physiological roles of an enhancer cluster in the SCN5A-SCN10A locus, show that it controls the chromatin architecture of the locus and Scn5a expression, and suggest that genetic variants affecting its activity may influence cardiac function.

scholarly journals Identification of a unique splicing regulatory cluster in hepatitis B virus pregenomic RNA

FEBS Letters ◽  
2011 ◽  
Vol 585 (20) ◽  
pp. 3348-3353 ◽  
Author(s):  
Joydip Bhanja Chowdhury ◽  
Dipika Roy ◽  
Sagarmoy Ghosh
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
B Virus ◽  
Regulatory Cluster

scholarly journals Evolutionary emergence of the rac3b/rfng/sgca regulatory cluster refined mechanisms for hindbrain boundaries formation

2018 ◽  
Vol 115 (16) ◽  
pp. E3731-E3740 ◽  
Author(s):  
Joaquín Letelier ◽  
Javier Terriente ◽  
Ivan Belzunce ◽  
Adria Voltes ◽  
Cristian Alberto Undurraga ◽  
...  
Keyword(s):  
Selective Advantage ◽  
Regulatory Elements ◽  
Small Gtpase ◽  
The Novel ◽  
Tissue Architecture ◽  
Regulatory Interactions ◽  
Cable Assembly ◽  
Evolutionary Emergence ◽  
Regulatory Cluster

Developmental programs often rely on parallel morphogenetic mechanisms that guarantee precise tissue architecture. While redundancy constitutes an obvious selective advantage, little is known on how novel morphogenetic mechanisms emerge during evolution. In zebrafish, rhombomeric boundaries behave as an elastic barrier, preventing cell intermingling between adjacent compartments. Here, we identify the fundamental role of the small-GTPase Rac3b in actomyosin cable assembly at hindbrain boundaries. We show that the novel rac3b/rfng/sgca regulatory cluster, which is specifically expressed at the boundaries, emerged in the Ostariophysi superorder by chromosomal rearrangement that generated new cis-regulatory interactions. By combining 4C-seq, ATAC-seq, transgenesis, and CRISPR-induced deletions, we characterized this regulatory domain, identifying hindbrain boundary-specific cis-regulatory elements. Our results suggest that the capacity of boundaries to act as an elastic mesh for segregating rhombomeric cells evolved by cooption of critical genes to a novel regulatory block, refining the mechanisms for hindbrain segmentation.

scholarly journals STEM-31. TARGETING GLIOBLASTOMA STEM CELLS BY PERTURBING A NOVEL GENE REGULATORY CLUSTER TO REDUCE TUMOR RECURRENCE

2017 ◽  
Vol 19 (suppl_6) ◽  
pp. vi232-vi232
Author(s):  
Changwang Deng ◽  
Mathew Sebastian ◽  
Son Le ◽  
Dongjiang Chen ◽  
Nagheme Thomas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tumor Recurrence ◽  
Glioblastoma Stem Cells ◽  
Novel Gene ◽  
Gene Regulatory ◽  
Regulatory Cluster

scholarly journals An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 175 ◽  
Author(s):  
Chen Cheng ◽  
Kil-Young Yun ◽  
Habtom W Ressom ◽  
Bijayalaxmi Mohanty ◽  
Vladimir B Bajic ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Low Temperature ◽  
Early Response ◽  
Japonica Rice ◽  
Regulatory Cluster
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