Female accessory glands and sperm reception in Tingidae (Heteroptera)

1990 ◽  
Vol 99 (6) ◽  
pp. 431-446
Author(s):  
David Livingstone ◽  
M H S Yacoob
Keyword(s):  
1959 ◽  
Vol 100 (3) ◽  
pp. 540-543 ◽  
Author(s):  
S. C. Lyster ◽  
G. H. Lund ◽  
W. E. Dulin ◽  
R. O. Stafford

2005 ◽  
Vol 266 (1) ◽  
pp. 95-104 ◽  
Author(s):  
D. A. Taggart ◽  
G. A. Shimmin ◽  
J. R. Ratcliff ◽  
V. R. Steele ◽  
R. Dibben ◽  
...  

2007 ◽  
Vol 69 (11) ◽  
pp. 1109-1116 ◽  
Author(s):  
Francesca T. CANNIZZO ◽  
Gabriele ZANCANARO ◽  
Francesca SPADA ◽  
Chiara MULASSO ◽  
Bartolomeo BIOLATTI

Reproduction ◽  
2002 ◽  
pp. 491-499 ◽  
Author(s):  
H Chen ◽  
MP Cheung ◽  
PH Chow ◽  
AL Cheung ◽  
W Liu ◽  
...  

Reactive oxygen species scavengers present in male accessory sex gland secretions might afford antioxidant protection to sperm DNA. This study was conducted to determine whether accessory sex gland secretions protect the genome and function of spermatozoa against oxidative damage in the uterus. Male golden hamsters were divided into four experimental groups: (i) all accessory sex glands removed; (ii) ampullary glands removed; (iii) ventral prostate gland removed and (iv) sham-operated controls. Ejaculated spermatozoa recovered from uteri 15-30 min after mating with experimental males and caput and cauda epididymal spermatozoa obtained from intact males were incubated in 0-20 mmol NADPH l(-1) for 2 h. These spermatozoa and untreated uterine spermatozoa were processed for two types of comet assay (single cell gel electrophoresis): alkaline comet assay (pH > 13) which revealed single-strand DNA breakage and neutral comet assay (pH 9) which revealed double-strand DNA breakage. In comparison with the sham-operated controls, spermatozoa that had not been exposed to accessory sex gland secretions had a higher incidence and more extensive single-strand DNA damage with increasing concentrations of NADPH. Spermatozoa from hamsters without ampullary glands and from hamsters without the ventral prostate glands were similar to those of the control group. After incubation with NADPH, the capacity of spermatozoa from hamsters without accessory glands and from sham-operated controls to fuse with oocytes in vitro was reduced. However, only hamsters without accessory glands showed a negative correlation between single-strand DNA damage and sperm-oocyte fusion. Cauda epididymal spermatozoa were less susceptible to NADPH treatment compared with caput epididymal spermatozoa. The results of the present study showed that male accessory sex gland secretions can preserve the integrity of the sperm genome.


1933 ◽  
Vol s2-76 (301) ◽  
pp. 35-61
Author(s):  
DEV RAJ MEHTA

A general account of the internal reproductive organs and the external genitalia and their development is given. The ‘penis lobes’ develop earlier than the ‘valvae lobes’, and independently of them. The tegumen is the modified ninth tergite. The tenth segment is visibly distinguished into a tergal and sternal part in the pupal stages, and the anal tube passes between the two sclerites. The uncus and the gnathos are dorsal and ventral processes respectively of the tenth segment. The anellus lobes develop as lateral processes of the ninth sternite on either side of the penis. The vasa deferentia during larval life do not extend beyond the eighth abdominal segment and lie in a latero-ventral position. They meet the extensions from the ectodermal ‘ductus ejaculatorius duplex’ during the last larval stadium. From the earliest caterpillar stage there exists a pair of ectodermal ducts formed by the differentiation of the epidermis on the ninth sternite. Towards the end of larval life they divide by constriction to form the accessory glands and the ‘ductus ejaculatory duplex’. At this stage they extend on either side to meet the vasa deferentia. The vesiculae seminales develop by distension from the region of junction between the vasa deferentia and the proximal portion of the ejaculatory duct. The ‘ductus ejaculatorius simplex’ arises as an ectodermal invagination between the pair of ‘penis lobes’ during the final larval instar. It is established that, with the exception of the vasa deferentia, all the remaining elements in the efferent genital system are derived from the ectoderm.


1948 ◽  
Vol s3-89 (7) ◽  
pp. 291-332
Author(s):  
A. D. LESS ◽  
J.W. L. BEAMENT

1. During the oviposition of ticks a glandular organ--the organ of Géné is everted and touches the egg. If it is prevented from everting most of the eggs shrivel rapidly; few hatch even in a humid atmosphere. 2. The waterproofing properties of the normal egg are conferred by a superficial coating of wax, 0.5-2.0 µ. in thickness. In Ornithodorus moubata the wax is secreted and applied solely by Géné's organ. In Ixodes ricinus waterproofing takes place in two stages: an incomplete covering of wax, probably secreted by the lobed accessory glands, is first smeared over the egg during its passage down the vagina; waterproofing is then completed by a further application of wax from Géné's organ after the egg has been laid. Owing to its superficial position on the egg the wax layer is readily attacked by solvents and emulsifiers. 3. The morphology of Géné's organ in O. moubata is described. The gland is a proliferation of the epidermis which lies detached from the cuticle. Its secretion, a watery refractile liquid containing the wax precursor, accumulates between the gland and the cuticle in two horn-like extensions. The wax is probably secreted through pore canals distributed over a narrow zone of cuticle below the horns; the cement covering-layer of the epicuticle does not extend to this zone. 4. The transparent, heat-stable material isolated from the horns of Géné's organ is regarded as the wax precursor. Solubility in water is probably con ferred by chemical linkage with protein. The precursor is taken up from the horns, where it is stored, and is presumably broken down within the gland cells. The wax is then secreted through the pore canals while the protein moiety is retained by the cell. 5. The critical temperatures of the eggs of Ixodidae range from 35° C. in I. ricinus to 44° C. in Hyalomma savignyi; only slightly higher critical temperatures were recorded for Argasidae (45° C. in O. moubata). Eggs with lower critical temperatures are more susceptible to desiccation. The susceptibility of the eggs of a given species is of the same order as that of the parent species; but whereas in Ixodidae the critical temperatures of the egg and the cuticle of the female tick are approximately the same, in Argasidae the critical temperatures of the cuticle are much higher (62° C. in O. moubata). These differences are related to the physical properties of the waxes. The cuticular wax in O. moubata is hard and crystalline (m.p. 65° C), whereas the egg wax is soft and viscous (m.p. 50-54° C). 6. The natural wax from Géné's organ has definite powers of spreading on the surface of the egg and so completing the waterproofing layer. 7. The material extracted with boiling chloroform from egg-shells or from nymphal cuticles separates spontaneously into two fractions, a hard white wax (c. 85 per cent, by weight) and a soft yellow grease (c. 15 per cent.). The properties of these two lipoids differ conspicuously from those of the natural wax. Attempts to deposit the extracted materials on membranes in the form of a waterproofing layer were unsuccessful. 8. Ovulation is described in O. moubata. The shell of the tick egg is secreted by the oocyte itself and not by follicle cells. Three layers can be distinguished in the 24-hour egg: (i) an outer wax layer; (ii) an incomplete layer of granules which reduce ammoniacal silver nitrate; (iii) a shell layer. A fourth layer, the inner membrane (iv), is secreted by the oocyte after incubation for 2-3 days. 9. Both the shell layer and the inner membrane are composed of resistant, elastic protein and are devoid of chitin. The shell layer of the unwaterproofed egg is highly permeable to water and to large molecules with either hydrophilic or lipophilic affinities. The inner membrane is at first freely permeable to water and to inorganic ions. During the course of incubation the wax gradually migrates into the shell material and may reach the inner membrane. As this occurs, the effectiveness of abrasive dusts and of chloroform in promoting increased transpiration through the shell is notably reduced.


2001 ◽  
Vol 267 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Pedro P. López ◽  
Juán F. Santarén ◽  
M.Fernanda Ruiz ◽  
Pedro Esponda ◽  
Lucas Sánchez

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