Production of Polyclonal Antibodies Using Recombinant Coat Protein of Papaya ringspot virus and their Use in Immunodiagnosis

2008 ◽  
Vol 18 (1) ◽  
pp. 109-111 ◽  
Author(s):  
Surekha Agarwal ◽  
M. Krishna Reddy ◽  
R. K. Jain
Keyword(s):  
Coat Protein ◽  
Polyclonal Antibodies ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Recombinant Coat Protein

scholarly journals Engineered Resistance Against Papaya ringspot virus in Venezuelan Transgenic Papayas

Plant Disease ◽  
2004 ◽  
Vol 88 (5) ◽  
pp. 516-522 ◽  
Author(s):  
Gustavo Fermin ◽  
Valentina Inglessis ◽  
Cesar Garboza ◽  
Sairo Rangel ◽  
Manuel Dagert ◽  
...  
Keyword(s):  
Amino Acid ◽  
Agrobacterium Tumefaciens ◽  
Coat Protein ◽  
Amino Acid Sequence ◽  
Sequence Similarity ◽  
Ringspot Virus ◽  
Amino Acid Sequence Similarity ◽  
Papaya Ringspot Virus ◽  
Local Varieties ◽  
Cp Gene

Local varieties of papaya grown in the Andean foothills of Mérida, Venezuela, were transformed independently with the coat protein (CP) gene from two different geographical Papaya ringspot virus (PRSV) isolates, designated VE and LA, via Agrobacterium tumefaciens. The CP genes of both PRSV isolates show 92 and 96% nucleotide and amino acid sequence similarity, respectively. Four PRSV-resistant R0 plants were intercrossed or selfed, and the progenies were tested for resistance against the homologous isolates VE and LA, and the heterologous isolates HA (Hawaii) and TH (Thailand) in greenhouse conditions. Resistance was affected by sequence similarity between the transgenes and the challenge viruses: resistance values were higher for plants challenged with the homologous isolates (92 to 100% similarity) than with the Hawaiian (94% similarity) and, lastly, Thailand isolates (88 to 89% similarity). Our results show that PRSV CP gene effectively protects local varieties of papaya against homologous and heterologous isolates of PRSV.

Virus Resistant Papaya Plants Derived from Tissues Bombarded with the Coat Protein Gene of Papaya Ringspot Virus

1992 ◽  
Vol 10 (11) ◽  
pp. 1466-1472 ◽  
Author(s):  
Maureen M. M. Fitch ◽  
Richard M. Manshardt ◽  
Dennis Gonsalves ◽  
Jerry L. Slightom ◽  
John C. Sanford
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

Allergenicity Assessment of the Papaya Ringspot Virus Coat Protein Expressed in Transgenic Rainbow Papaya

2011 ◽  
Vol 59 (18) ◽  
pp. 10006-10012 ◽  
Author(s):  
Gustavo Fermín ◽  
Ronald C. Keith ◽  
Jon Y. Suzuki ◽  
Stephen A. Ferreira ◽  
Douglas A. Gaskill ◽  
...  
Keyword(s):  
Coat Protein ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Virus Coat Protein ◽  
Virus Coat

scholarly journals Genetic variability of Papaya ringspot virus isolates in Norte de Santander - Colombia

2015 ◽  
Vol 33 (2) ◽  
pp. 184-193 ◽  
Author(s):  
Giovanni Chaves-Bedoya ◽  
Luz Yineth Ortiz-Rojas
Keyword(s):  
Coat Protein ◽  
Genetic Variability ◽  
Carica Papaya ◽  
Local Level ◽  
Bioinformatic Analysis ◽  
Ringspot Virus ◽  
Limiting Factor ◽  
Papaya Ringspot Virus ◽  
Phylogenetic Groups ◽  
Valle Del Cauca

The Papaya ringspot virus (PRSV), a member of the potyvirus that is transmitted by aphids within the Potyviridae family, is the main limiting factor for papaya (Carica papaya L.) and Cucurbits worldwide and causes losses of up to 100%. In this study, we conducted research on the genetic diversity of PRSV isolates collected from two locations in the department of Norte de Santander, Colombia. The analysis was performed by comparing the nucleotide sequences of the region that encode the coat protein (CP) of nine PRSV isolates from the Villa del Rosario location and 12 isolates from the Campo Hermoso location. The analysis included three sequences of the CP of PRSV isolates reported in the Colombian departments of Arauca and Valle del Cauca. The bioinformatic analysis suggested that the PRSV isolates from the locations in Norte de Santander were different from each other, grouping into different phylogenetic groups. Anexistence of recombination events in the coat protein was observed. This is the first study of PRSV genetic variability that has been conducted at the local level in Colombia.

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