Decreased expression of insulin-sensitive glucose transporter mRNA (GLUT-4) in adipose tissue of non-insulin-dependent diabetic and obese patients: Evaluation by a simplified quantitative PCR assay

1994 ◽  
Vol 17 (9) ◽  
pp. 709-715 ◽  
Author(s):  
G. Giacchetti ◽  
E. Faloia ◽  
A. Taccaliti ◽  
P. P. Morosini ◽  
G. Arnaldi ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Quantitative Pcr ◽  
Glucose Transporter ◽  
Obese Patients ◽  
Pcr Assay ◽  
Glut 4 ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic ◽  
Glucose Transporter Mrna ◽  
Quantitative Pcr Assay

scholarly journals Insulin receptor and insulin-responsive glucose transporter (GLUT 4) mutations and polymorphisms in a welsh type 2 (non-insulin-dependent) diabetic population

Diabetologia ◽  
1992 ◽  
Vol 35 (5) ◽  
pp. 486-489 ◽  
Author(s):  
S. O'Rahilly ◽  
A. Krook ◽  
R. Morgan ◽  
A. Reese ◽  
J. S. Flier ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
Glucose Transporter ◽  
Glut 4 ◽  
Insulin Dependent ◽  
Diabetic Population ◽  
Insulin Dependent Diabetic

scholarly journals Glucose transporter expression and glucose utilization in skeletal muscle and brown adipose tissue during starvation and re-feeding

1992 ◽  
Vol 282 (1) ◽  
pp. 231-235 ◽  
Author(s):  
D M Smith ◽  
S R Bloom ◽  
M C Sugden ◽  
M J Holness
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Tibialis Anterior ◽  
Glucose Transporter ◽  
Glucose Utilization ◽  
Interscapular Brown Adipose Tissue ◽  
Glut 4 ◽  
Mrna Concentration ◽  
Brown Adipose ◽  
Transporter Expression

Starvation (48 h) decreased the concentration of mRNA of the insulin-responsive glucose transporter isoform (GLUT 4) in interscapular brown adipose tissue (IBAT) (56%) and tibialis anterior (10%). Despite dramatic [7-fold (tibialis anterior) and 40-fold (IBAT)] increases in glucose utilization after 2 and 4 h of chow re-feeding, no significant changes in GLUT 4 mRNA concentration were observed in these tissues over this re-feeding period. The results exclude changes in GLUT 4 mRNA concentration in mediating the responses of glucose transport in these tissues to acute re-feeding after prolonged starvation.

scholarly journals Molecular regulation of GLUT-4 targeting in 3T3-L1 adipocytes.

1995 ◽  
Vol 130 (5) ◽  
pp. 1081-1091 ◽  
Author(s):  
B J Marsh ◽  
R A Alm ◽  
S R McIntosh ◽  
D E James
Keyword(s):  
Cell Surface ◽  
Glucose Transporter ◽  
Subcellular Fractionation ◽  
Molecular Regulation ◽  
Surface Distribution ◽  
Amino Terminal ◽  
Glut 4 ◽  
Dileucine Motif ◽  
A Cell ◽  
Insulin Dependent

Insulin stimulates glucose transport in muscle and adipose tissue by triggering the movement of the glucose transporter GLUT-4 from an intracellular compartment to the cell surface. Fundamental to this process is the intracellular sequestration of GLUT-4 in nonstimulated cells. Two distinct targeting motifs in the amino and carboxy termini of GLUT-4 have been previously identified by expressing chimeras comprised of portions of GLUT-4 and GLUT-1, a transporter isoform that is constitutively targeted to the cell surface, in heterologous cells. These motifs-FQQI in the NH2 terminus and LL in the COOH terminus-resemble endocytic signals that have been described in other proteins. In the present study we have investigated the roles of these motifs in GLUT-4 targeting in insulin-sensitive cells. Epitope-tagged GLUT-4 constructs engineered to differentiate between endogenous and transfected GLUT-4 were stably expressed in 3T3-L1 adipocytes. Targeting was assessed in cells incubated in the presence or absence of insulin by subcellular fractionation. The targeting of epitope-tagged GLUT-4 was indistinguishable from endogenous GLUT-4. Mutation of the FQQI motif (F5 to A5) caused GLUT-4 to constitutively accumulate at the cell surface regardless of expression level. Mutation of the dileucine motif (L489L490 to A489A490) caused an increase in cell surface distribution only at higher levels of expression, but the overall cells surface distribution of this mutant was less than that of the amino-terminal mutants. Both NH2- and COOH-terminal mutants retained insulin-dependent movement from an intracellular to a cell surface locale, suggesting that neither of these motifs is involved in the insulin-dependent redistribution of GLUT-4. We conclude that the phenylalanine-based NH2-terminal and the dileucine-based COOH-terminal motifs play important and distinct roles in GLUT-4 targeting in 3T3-L1 adipocytes.

Expression of GLUT4 Glucose Transporter mRNA and Protein in Skeletal Muscle and Adipose Tissue from Rats in Late Pregnancy

1993 ◽  
Vol 191 (2) ◽  
pp. 405-412 ◽  
Author(s):  
S. Okuno ◽  
Y. Maeda ◽  
Y. Yamaguchi ◽  
Y. Takao ◽  
R.A. Trocino ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Glucose Transporter ◽  
Late Pregnancy ◽  
Glucose Transporter Mrna

scholarly journals Development and Validation of a Sensitive and Specific sodB-Based Quantitative PCR Assay for Molecular Detection of Ehrlichia Species

2014 ◽  
Vol 52 (11) ◽  
pp. 4030-4032 ◽  
Author(s):  
B. A. Qurollo ◽  
D. Riggins ◽  
A. Comyn ◽  
M. T. Zewde ◽  
E. B. Breitschwerdt
Keyword(s):  
Quantitative Pcr ◽  
Molecular Detection ◽  
Pcr Assay ◽  
Development And Validation ◽  
Quantitative Pcr Assay
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