A factorial approach for a sugarcane juice-based low cost culture medium: increasing the astaxanthin production by the red yeast Phaffia rhodozyma

1998 ◽  
Vol 19 (3) ◽  
pp. 161-164 ◽  
Author(s):  
J. A. Florêncio ◽  
C. R. Soccol ◽  
L. F. Furlanetto ◽  
T. M. B. Bonfim ◽  
N. Krieger ◽  
...  
Keyword(s):  
Culture Medium ◽  
Low Cost ◽  
Phaffia Rhodozyma ◽  
Sugarcane Juice ◽  
Factorial Approach ◽  
Red Yeast ◽  
Astaxanthin Production

A factorial approach for a sugarcane juice-based low cost culture medium: increasing the astaxanthin production by the red yeast

1998 ◽  
Vol 19 (3) ◽  
pp. 161 ◽  
Author(s):  
J. A. Florêncio ◽  
C. R. Soccol ◽  
L. F. Furlanetto ◽  
T. M. B. Bonfim ◽  
N. Krieger ◽  
...  
Keyword(s):  
Culture Medium ◽  
Low Cost ◽  
Sugarcane Juice ◽  
Factorial Approach ◽  
Red Yeast ◽  
Astaxanthin Production

scholarly journals Effect of feeding methods on the astaxanthin production by Phaffia rhodozyma in fed-batch process

2005 ◽  
Vol 48 (3) ◽  
pp. 397-401 ◽  
Author(s):  
Danilo Gomes Moriel ◽  
Miriam Blumel Chociai ◽  
Iara Maria Pereira Machado ◽  
José Domingos Fontana ◽  
Tania Maria Bordin Bonfim
Keyword(s):  
Low Cost ◽  
Biomass Productivity ◽  
Batch Process ◽  
Phaffia Rhodozyma ◽  
Batch Processes ◽  
Fed Batch ◽  
Sugar Cane Juice ◽  
Astaxanthin Production ◽  
Feeding Methods ◽  
Effect Of Feeding

The effect of feeding methods on the production of astaxanthin by the yeast Phaffia rhodozyma ATCC 24202 was studied, using continuous and pulsed fed-batch processes and low cost materials as substrates (sugar cane juice and urea). In continuous fed-batch processes, a cellular astaxanthin concentration of 383.73 µg/g biomass was obtained. But in pulsed fed-batch processes a reduction in the cellular astaxanthin concentration (303.34 µg/g biomass) was observed. Thus the continuous fed-batch processes could be an alternative to industrial production of astaxanthin, allowing an increase in the biomass productivity without losses on astaxanthin production by the yeast.

Bioconversion of low-cost brewery waste to biosurfactant: an improvement of surfactin production by culture medium optimization

2021 ◽  
pp. 108058
Author(s):  
Talita Corrêa Nazareth ◽  
Conrado Planas Zanutto ◽  
Danielle Maass ◽  
Antônio Augusto Ulson de Souza ◽  
Selene Maria de Arruda Guelli Ulson de Souza
Keyword(s):  
Culture Medium ◽  
Medium Optimization ◽  
Low Cost ◽  
Culture Medium Optimization ◽  
Surfactin Production

scholarly journals “CLUPS”: A New Culture Medium for the Axenic Growth ofEntamoeba histolytica

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
F. Gonzalez-Salazar ◽  
I. Meester ◽  
F. J. Guzmán De La Garza ◽  
L. H. De La Garza-Salinas ◽  
A. Sampayo-Reyes ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
The Novel ◽  
Phospholipase Activity ◽  
Major Health Problem ◽  
Beef Liver ◽  
Major Health ◽  
Liver Abscesses ◽  
Therapeutic Tools

Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium forEntamoeba histolyticawhich allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS),E. histolyticatrophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters.E. histolyticatrophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses.

Analysis of relationship between key genes and astaxanthin biosynthesis in Phaffia rhodozyma by transcript level and gene knock-out

2019 ◽  
Author(s):  
Zhipeng Li ◽  
Lina Chen ◽  
Tianli Li ◽  
Xiping Du ◽  
Ning He ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Transcript Level ◽  
Phaffia Rhodozyma ◽  
Knock Out ◽  
Astaxanthin Production ◽  
Key Genes ◽  
Different Strains ◽  
The Relationship ◽  
Rate Limiting ◽  
Exact Relationship

Abstract Background Phaffia rhodozyma is a potential industrial source for production of natural astaxanthin. The synthetic mechanism of astaxanthin in P. rhodozyma is complex and unclear that blocked its development. Results In this study, eight genes related to dicyclic and monocyclic pathway in three different strains of P. rhodozyma were analyzed, and the relationship between the expression and astaxanthin biosynthesis was explored. Among these genes, crtYB (R=0.75, P<0.05) and asy genes (R=0.74, P<0.05) showed the most closely correlation with astaxanthin biosynthesis. In order to further study exact relationship, crtYB and asy genes were knocked out by homologous recombination. After crtYB knock-out, astaxanthin was decreased to be under detected line. It suggested crtYB played a role in dicyclic and monocyclic pathway. Meanwhile, the asy gene was in dicyclic pathway of astaxanthin biosynthesis, and its knock-out would promote the astaxanthin biosynthesis in monocyclic pathway, resulting in a 25.04% increase in astaxanthin production. Conclusion The possible rate-limiting enzymes were asy gene and crtYB illustrated by analysis of regression. Knock-out of asy and crtYB gene was great helpful to understand the synthetic pathway of astaxanthin, and significant to the industrial application of producing astaxanthin.

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