scholarly journals “CLUPS”: A New Culture Medium for the Axenic Growth ofEntamoeba histolytica

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
F. Gonzalez-Salazar ◽  
I. Meester ◽  
F. J. Guzmán De La Garza ◽  
L. H. De La Garza-Salinas ◽  
A. Sampayo-Reyes ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
The Novel ◽  
Phospholipase Activity ◽  
Major Health Problem ◽  
Beef Liver ◽  
Major Health ◽  
Liver Abscesses ◽  
Therapeutic Tools

Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium forEntamoeba histolyticawhich allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS),E. histolyticatrophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters.E. histolyticatrophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses.

scholarly journals Regulation of Exosomes in the Pathogenesis of Breast Cancer

2021 ◽  
Author(s):  
Congjian Shi ◽  
Hongqin Yang ◽  
Zhengchao Wang ◽  
Zhenghong Zhang
Keyword(s):  
Breast Cancer ◽  
Lipid Bilayers ◽  
Cell Types ◽  
Cancer Diagnostics ◽  
Major Health Problem ◽  
Major Health ◽  
Diagnostic Strategies ◽  
Therapeutic Tools ◽  
Common Malignancy

Extracellular vesicles (EVs) are a heterogeneous group of endogenous nanoscale vesicles that are secreted by various cell types. Based on their biogenesis and size distribution, EVs can be broadly classified as exosomes and microvesicles. Exosomes are enveloped by lipid bilayers with a size of 30–150 nm in diameter, which contain diverse biomolecules, including lipids, proteins and nucleic acids. Exosomes transport their bioactive cargoes from original cells to recipient cells, thus play crucial roles in mediating intercellular communication. Breast cancer is the most common malignancy among women and remains a major health problem worldwide, diagnostic strategies and therapies aimed at breast cancer are still limited. Growing evidence shows that exosomes are involved in the pathogenesis of breast cancer, including tumorigenesis, invasion and metastasis. Here, we provide a straightforward overview of exosomes and highlight the role of exosomes in the pathogenesis of breast cancer, moreover, we discuss the potential application of exosomes as biomarkers and therapeutic tools in breast cancer diagnostics and therapeutics.

Use of cheese whey for biomass production and spray drying of probiotic lactobacilli

2014 ◽  
Vol 81 (3) ◽  
pp. 267-274 ◽  
Author(s):  
Luisina Lavari ◽  
Roxana Páez ◽  
Alejandra Cuatrin ◽  
Jorge Reinheimer ◽  
Gabriel Vinderola
Keyword(s):  
Spray Drying ◽  
Culture Medium ◽  
Cheese Whey ◽  
Culture Media ◽  
Low Cost ◽  
Starch Solution ◽  
Surface Response Methodology ◽  
Promising Candidate ◽  
Available Technology ◽  
Probiotic Lactobacilli

The double use of cheese whey (culture medium and thermoprotectant for spray drying of lactobacilli) was explored in this study for adding value to this wastewater. In-house formulated broth (similar to MRS) and dairy media (cheese and ricotta whey and whey permeate) were assessed for their capacity to produce biomass of Lactobacillus paracasei JP1, Lb. rhamnosus 64 and Lb. gasseri 37. Simultaneously, spray drying of cheese whey-starch solution (without lactobacilli cells) was optimised using surface response methodology. Cell suspensions of the lactobacilli, produced in in house-formulated broth, were spray-dried in cheese whey-starch solution and viability monitored throughout the storage of powders for 2 months. Lb. rhamnosus 64 was able to grow satisfactorily in at least two of the in-house formulated culture media and in the dairy media assessed. It also performed well in spray drying. The performance of the other strains was less satisfactory. The growth capacity, the resistance to spray drying in cheese whey-starch solution and the negligible lost in viability during the storage (2 months), makes Lb. rhamnosus 64 a promising candidate for further technological studies for developing a probiotic dehydrated culture for foods, utilising wastewaters of the dairy industry (as growth substrate and protectant) and spray drying (a low-cost widely-available technology).

scholarly journals Comparison between two culture media for in vitro evaluation of antifungal susceptibility of the Sporothrix schenckii complex

2012 ◽  
Vol 87 (4) ◽  
pp. 561-565 ◽  
Author(s):  
Cheila Denise Ottonelli Stopiglia ◽  
Daiane Péres Marchese ◽  
Daiane Heidrich ◽  
Julia Medeiros Sorrentino ◽  
Fabiane Jamono Vieira ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
Antifungal Susceptibility ◽  
Sporothrix Schenckii ◽  
Roswell Park Memorial Institute ◽  
Broth Culture ◽  
In Vitro Evaluation

BACKGROUND: The standard methodology for determining the antifungal sensitivity against the Sporothrix schenckii complex recommends the use of the 1640 Roswell Park Memorial Institute culture medium (RPMI) buffered with morpholinepropanolsulfonic acid (MOPS). However, while this is a high-cost medium which requires a laborious implementation and sterilization by filtration, the Sabouraud dextrose broth is a low-cost medium, widely used in mycology, sterilized by autoclave. OBJECTIVE: To evaluate the performance of the Sabouraud dextrose broth culture medium as a substitute for the RPMI 1640-MOPS in determining the antifungal sensitivity of S. schenckii. METHODS: Forty-eight clinical isolates were evaluated against five antifungal agents: itraconazole, ketoconazole, fluconazole, amphotericin B and terbinafine, using the method of broth microdilution advocated by the M38-A2 protocol of the Clinical and Laboratory Standards Institute. RESULTS: There were no significant differences between the Minimum Inhibitory Concentrations obtained in the two culture media for all the antifungals, with the exception of the amphotericin B. Regarding this drug, the Minimum Inhibitory Concentration range obtained were wider for the Sabouraud dextrose broth than for the Roswell Park Memorial Institute morpholinepropanelsulfonic acid. CONCLUSIONS: The Sabouraud dextrose broth showed potential to be used in the in vitro evaluation of the S. schenckii complex antifungal activity.

scholarly journals Low-cost culture medium for biomass production of lactic acid bacteria with probiotic potential destined to broilers

2020 ◽  
Vol 20 (1) ◽  
pp. 1-9
Author(s):  
Ayelen P. BERISVIL ◽  
Diego M. ASTESANA ◽  
Jorge A. ZIMMERMANN ◽  
Laureano S. FRIZZO ◽  
Eugenia ROSSLER ◽  
...  
Keyword(s):  
Biomass Production ◽  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
Probiotic Potential ◽  
Cost Growth ◽  
Commercial Medium ◽  
Mg Addition ◽  
The Cost ◽  
Similar Growth

The aim of this study was to evaluate different low-cost culture media for biomass production of 3 potential probiotic L. salivarius strains, which could be destined to broilers at farms. Different formulated media based on whey permeate (WP) supplemented with nitrogenous sources were evaluated: yeast extract (YE), whey hydrolysate (WH) and MnSO4.H2O (Mn), MgSO4.7H2O (Mg). The growth of each strain in the formulated media and the cost was compared with their growth and cost in commercial medium (MRS). L. salivarius DSPV008P did not grow adequately in any of the formulated media. On the other hand, addition of YE and Mn in the formulated media increased L. salivarius DSPV002P and L. salivarius DPSV011P growth. In contrast, WH and Mg addition increased the L. salivarius DSPV002P biomass only. L. salivarius DSPV011P was the only strain that had similar growth performance in MRS as in the selected medium: WP + YE 8g/l + Mn. In this sense, L. salivarius DSPV011P reached a biomass of 9.22 Log (CFU/ml) in the selected formulated medium, with a low-cost growth medium 12 times less than in MRS. Although the effect of supplements added to the culture medium on kinetic parameters are strain dependent, L. salivarius DSPV011P is the strain with the best technological characteristics, capable of growing in a medium based on a by-product of the dairy industry supplemented with YE and Mn and at a much less cost than in MRS medium.

scholarly journals TEXTURIZED SOY PROTEIN AS AN ALTERNATIVE LOW-COST MEDIA FOR BACTERIA CULTIVATION

2020 ◽  
Vol 3 (4) ◽  
pp. 74-76
Author(s):  
Carlos Henrique da Silva Cruz ◽  
Jessica Bezerra dos Santos ◽  
Francielle Penha dos Santos ◽  
Gabriel Macedo Magalhães Silva ◽  
Eduardo Felipe Nascimento da Cruz ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Soy Protein ◽  
Production Cost ◽  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
Educational Institutions ◽  
Practical Teaching

Most of the culture media used in bacterial growth is composed of complex ingredients, increasing the value of the product. This makes its acquisition unavailable by educational institutions without sufficient funding, making even more difficult the practical teaching of microbiology. Therefore, the development of an alternative medium of simple composition and low-cost becomes necessary. This work aimed to use texturized soy protein (TSP) as a low cost culture medium that allows the bacterial growth. For the composition of the broths, concentrations between 0.5% and 10% were prepared. Thirty-eight bacteria, including important pathogens associated with food, were inoculated and the concentration of 7.5% TSP allowed the growth of 100% of the tested bacteria, with a production cost of approximately 86% and 68% lower than tryptic soy broth and agar, respectively. This work demonstrates that the use of a culture medium of easy acquisition and low cost is feasible and has good results.

scholarly journals Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte

2017 ◽  
Vol 29 (0) ◽  
Author(s):  
Lúcia Helena Sipaúba-Tavares ◽  
Alexandra Maria Donadon Lusser Segali ◽  
Flavia Almeida Berchielli-Morais ◽  
Bruno Scardoeli-Truzzi
Keyword(s):  
Growth Rate ◽  
Eichhornia Crassipes ◽  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
Nutritional Composition ◽  
Inorganic Fertilizer ◽  
Lipid Contents ◽  
Commercial Medium ◽  
The Media

Abstract Aim: The influence of three different culture media on the growth and biochemical composition of Ankistrodemsus gracilis microalgae is evaluated. Methods Microalgae were placed in a bath culture during 28 days, with inorganic fertilizer (NPK) and macrophyte Eichhornia crassipes as culture medium to compare the growth of microalgae and commercial medium CHU12. Protein and lipid contents, water conditions of the culture medium and physiological parameters were assessed weekly. Results Growth rate, macro-minerals, micro-nutrients and nutritional values were similar to commercial culture medium in the media with inorganic fertilizer and macrophyte. The biological indexes of microalgae were not influenced by weekly differences during the study period. Although lipid contents, nitrogen and growth rate of A. gracilis were higher in inorganic fertilizer and macrophyte media; Mn, B, Cu, S, dry matter, ash content, cell volume, TOC and total length were similar among the media used. The same high cell density 25 x 105 cells.mL-1 was obtained in NPK and in CHU12 medium respectively after 11 and 14 days cultivation. Conclusion Culture media inorganic fertilizer (NPK) and macrophyte (Eichhornia crassipes), were adequate and may replace the commercial medium CHU12 for the cultivation of microalgae A. gracilis, resulting in high nutritional composition, high biomass, high nutrients and low-cost.

scholarly journals Improvement of a Specific Culture Medium Based on Industrial Glucose for Carotenoid Production by Xanthophyllomyces dendrorhous

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 429
Author(s):  
Alejandro Torres-Haro ◽  
Anne Gschaedler ◽  
Juan C. Mateos-Díaz ◽  
Enrique J. Herrera-López ◽  
Rosa M. Camacho-Ruíz ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
Carotenoid Biosynthesis ◽  
Pilot Scale ◽  
P Value ◽  
Xanthophyllomyces Dendrorhous ◽  
Carotenoid Production ◽  
Yeast Peptone Dextrose ◽  
Specific Culture

In this study, a low-cost chemically defined (CD) culture medium was proposed and evaluated with the aim of replacing culture media such as yeast mold (YM) and yeast peptone dextrose (YPD), commonly used for growth and carotenoid production by Xanthophyllomyces dendrorhous. Initially, the CD culture medium was compared to the YM and YPD. The growth in optical density (OD) and carotenoid production (mg/L) of the cultures reached 4.88, 6.76, 5.79, and 0.67, 0.92, and 0.69, respectively. The CD culture served as the basis of an improved specific culture medium containing industrial glucose. Additionally, in this new formulation, vitamins, glutamate, and other compounds were evaluated. Industrial glucose more than doubled carotenoid production; however, the addition of vitamins was not essential for X. dendrorhous cultivation. Moreover, glutamate and Na2HPO4 proved to be highly significant factors (p-value < 0.05), increasing carotenoid biosynthesis from 0.67 to 1.33 mg/L. The specific culture was successfully used in a bioreactor at 2 L and 110 L pilot-scale levels, increasing carotenoid production up to 2 mg/L. It was demonstrated that the CD-specific culture medium is an efficient alternative to conventional culture media to carry out carotenoid production at the laboratory and pilot levels, with promising potential for industrial scaling.

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

Prevention and Treatment of Early Childhood Caries (ECC)

2016 ◽  
Vol 1 (3) ◽  
Author(s):  
Jeffrey .
Keyword(s):  
Early Childhood ◽  
Health Problem ◽  
Early Childhood Caries ◽  
Health Providers ◽  
Missing Teeth ◽  
Optimal Basis ◽  
Major Health Problem ◽  
Major Health ◽  
Childhood Caries

Early Childhood Caries (ECC) is a chronic disease that can be prevented. It commonlyaffects children involving in one or more decayed (with lesions or not) teeth, missing teeth (dueto caries), or teeth with fillings in children aged under 71 months. The disease is sometimesoverlooked, but this condition usually affects the general health of children. Early detection ofEarly Childhood Caries (ECC) can prevent problems which are harmful to children. Therefore,the ECC must be prevented and for teeth that have had dental caries they should be givenproper treatment so as not to worsen and affect the quality of life in children. Prevention of thisdisease is a significant component in any health program to prepare for the optimal basis forthe oral health of children. This condition will become a serious health problem if not handledproperly, and it is a major health problem for health providers throughout the world.Primarypreventive must be initiated since a woman getting pregnant.Keywords: Early Childhood Caries (ECC), prevention, treatment

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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