Mitochondrial DNA and AFLP markers support species status of Apis nigrocincta

AbstractThe genus Macroplea Samouelle, 1819 is a group of highly specialized aquatic leaf beetles occurring in the Palaearctic. Since the members of this genus are morphologically very similar, we addressed the question of species identification and delimitation by analysing the second half of the mitochondrial gene coding for the cytochrome oxidase I (COI) subunit. Species limits are inferred from the multimodal frequency distribution of genetic distances between specimens: low genetic distances within a species are clearly set apart from distances between species. The species status of the hitherto controversial species M. japana (Jacoby, 1885) is confirmed. The pattern of nucleotide and amino acid substitutions is discussed in the light of functional domains of the COI molecule. Although the data are preliminary, the results provide new data on the distribution of the species. Together with the phylogenetic analysis they allow for a discussion of the phylogeography of the genus.

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Drone flight times confirm the species status of Apis nigrocincta Smith, 1861 to be a species distinct from Apis cerana F, 1793, in Sulawesi, Indonesia

Apidologie ◽

10.1051/apido:19960504 ◽

1996 ◽

Vol 27 (5) ◽

pp. 361-369 ◽

Cited By ~ 19

Author(s):

S. Hadisoesilo ◽

G. W. Otis

Keyword(s):

Apis Cerana ◽

Species Status ◽

Apis Nigrocincta

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Mitochondrial DNA Variation, Species Limits, and Rapid Evolution of Plumage Coloration and Size in the Savannah Sparrow

The Condor ◽

10.1093/condor/107.1.21 ◽

2005 ◽

Vol 107 (1) ◽

pp. 21-28 ◽

Cited By ~ 1

Author(s):

Robert M. Zink ◽

James D. Rising ◽

Steve Mockford ◽

Andrew G. Horn ◽

Jonathan M. Wright ◽

...

Keyword(s):

Mitochondrial Dna ◽

Nova Scotia ◽

San Diego ◽

Baja California ◽

Rapid Evolution ◽

Plumage Coloration ◽

Phylogeographic Structure ◽

Species Status ◽

Passerculus Sandwichensis ◽

Savannah Sparrows

Abstract We compared sequences from two mitochondrial DNA (mtDNA) genes (ND2, ND3) in Savannah Sparrows (Passerculus sandwichensis; n = 112) sampled from Baja California (five sites), coastal Sonora and the continental range (eight sites). Populations from Baja California, San Diego and Sonora formed a clade within which there was no phylogeographic structure; this clade merits species status (Passerculus rostratus). The other clade, consisting of phenotypically “typical” savannah sparrows, should be classified as P. sandwichensis. Among the typical sparrows, there was no phylogeographic structure, although two major clades were discovered. Representatives of each of the two main clades occurred at most sampling localities, excluding Suisan Bay, California and Sable Island, Nova Scotia, Canada. Haplotypes found on Sable Island, representing the “Ipswich Sparrow,” were not distinctive, thereby failing to support species status for this taxon. On Isla San Benito, a single haplotype was found, which also occurred in other Mexican localities. The results for Sable Island and Isla San Benito show that size and plumage coloration can evolve rapidly. Variación en ADN Mitocondrial, Límites entre Especies y Evolución Rápida de la Coloración del Plumaje y el Tamaño en Passerculus sandwichensis Resumen. En este estudio comparamos secuencias de dos genes mitocondriales (ND2 y ND3) entre individuos de la especie Passerculus sandwichensis (n = 112) muestreados en Baja California (5 sitios), la costa de Sonora y el rango de distribución continental (8 sitios). Las poblaciones de Baja California, San Diego y Sonora formaron un clado, al interior del cual no existió estructura filogeográfica; este clado merece estatus de especie (Passerculus rostratus). El otro clado, conformado por individuos fenotípicamente “típicos”, debe clasificarse como P. sandwichensis. Entre los individuos típicos no existió estructura filogeográfica, aunque se descubrieron dos clados principales. Individuos representativos de cada uno de estos dos clados se encontraron en la mayoría de las localidades, excepto Suisan Bay, California y Sable Island, Nova Scotia. Los haplotipos encontrados en Sable Island, correspondientes al “gorrión de Ipswich” no fueron distintivos, lo que no apoya el estatus de especie para este taxón. En Isla San Benito se encontró un solo halpotipo, el cual también se encontraba en otras localidades mexicanas. Los resultados de Sable Island e Isla San Benito muestran que el tamaño y la coloración del plumaje pueden evolucionar rápidamente.

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Species Limits and Recent Population History in the Curve-Billed Thrasher

The Condor ◽

10.1093/condor/102.4.881 ◽

2000 ◽

Vol 102 (4) ◽

pp. 881-886 ◽

Cited By ~ 11

Author(s):

Robert M. Zink ◽

Rachelle C. Blackwell-Rago

Keyword(s):

Mitochondrial Dna ◽

Morphometric Analysis ◽

Sonoran Desert ◽

Population History ◽

Population Increase ◽

Phenotypic Characteristics ◽

Species Status ◽

Base Pairs ◽

Southern Group ◽

The Third

Abstract We surveyed 1,115 base pairs of mitochondrial DNA sequence from three gene regions in 66 Curve-billed Thrashers (Toxostoma curvirostre) taken from throughout their range. We found that populations sorted unambiguously into three groups. Two of these groups, the Curvirostre and Palmeri groups, have been known for many years because of their distinctive phenotypic characteristics, and their status was recently confirmed with an intensive morphometric analysis. The third (southern) group consists of populations in Puebla and Oaxaca, Mexico. The strong morphological and mtDNA distinctiveness of the Curvirostre and Palmeri groups suggests that they warrant species status. We recommend that more specimens of the southern group be obtained prior to formal taxonomic recognition of this form. Coalescence analyses suggest that the Curvirostre group has undergone a recent population increase, whereas the Palmeri group seems to have been more stable in its Sonoran Desert range.

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Taxonomic status of the black fly Prosimulium italicum Rivosecchi (Diptera: Simuliidae) based on genetic evidence

Zootaxa ◽

10.11646/zootaxa.4377.2.8 ◽

2018 ◽

Vol 4377 (2) ◽

pp. 280 ◽

Cited By ~ 1

Author(s):

MATÚŠ KÚDELA ◽

PETER H. ADLER ◽

TATIANA KÚDELOVÁ

Keyword(s):

Mitochondrial Dna ◽

Central Europe ◽

Sex Chromosomes ◽

Chromosome Banding ◽

Taxonomic Status ◽

Northern Europe ◽

Chromosome Polymorphism ◽

Species Status ◽

Banding Patterns ◽

Black Fly

The black fly Prosimulium italicum Rivosecchi, distributed in the Apennines and Sicily, was described as a subspecies of Prosimulium hirtipes (Fries), based on a few morphological details. It subsequently was considered conspecific with P. hirtipes and the name was synonymized. Analyses of polytene chromosome banding patterns and sequences of mitochondrial DNA (COI and COII) revealed deep genetic divergence between P. italicum from Italy and P. hirtipes from northern and central Europe and confirmed the species status of P. italicum. Populations of P. italicum either lack chromosomal inversion IS-9 or carry it as an X-chromosome polymorphism, whereas all analyzed populations of P. hirtipes (Slovakia, Sweden, England, and Scotland) are fixed for IS-9. The average K2P genetic distance was 3.7% between P. italicum and P. hirtipes from northern Europe (Sweden) and 4.3 % between P. italicum and P. hirtipes from central Europe (Slovakia). Cytogenetic analysis showed the presence of two cytoforms of P. hirtipes (‘A’ in Sweden and Slovakia and ‘B’ in England and Scotland) and two cytoforms of P. italicum (‘A’ in Sicily and ‘B’ in Campania and Basilicata), all of which differ in their sex chromosomes and autosomal polymorphisms, suggesting that P. hirtipes and P. italicum might each be a complex of cryptic species.

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