Construction of a binary BAC library for an apomictic monosomic addition line of Beta corolliflora in sugar beet and identification of the clones derived from the alien chromosome

2004 ◽  
Vol 108 (7) ◽  
pp. 1420-1425 ◽  
Author(s):  
Xiaohua Fang ◽  
Suhai Gu ◽  
Zhanyou Xu ◽  
Fan Chen ◽  
Dedong Guo ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Bac Library ◽  
Addition Line ◽  
Alien Chromosome ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Salt stress induced proteome and transcriptome changes in sugar beet monosomic addition line M14

2012 ◽  
Vol 169 (9) ◽  
pp. 839-850 ◽  
Author(s):  
Le Yang ◽  
Chunquan Ma ◽  
Linlin Wang ◽  
Sixue Chen ◽  
Haiying Li
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Mitotic karyotypes of Brassica campestris and Brassica alboglabra and identification of the B. alboglabra chromosome in an addition line

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 313-319 ◽  
Author(s):  
B. F. Cheng ◽  
W. K. Heneen ◽  
B. Y. Chen
Keyword(s):  
Brassica Campestris ◽  
Differential Staining ◽  
Gene Localization ◽  
Addition Line ◽  
Alien Chromosome ◽  
Brassica Alboglabra ◽  
Large Size ◽  
C Genome ◽  
Monosomic Addition ◽  
Monosomic Addition Line

A Brassica campestris–alboglabra monosomic addition line (genome: AA + one chromosome from the C genome, 2n = 21) harbours the Brassica alboglabra (CC, 2n = 18) chromosome with the gene for erucic acid. In order to identify this chromosome, we have studied the mitotic prometaphase chromosomes of Brassica campestris (AA, 2n = 20), B. alboglabra, and the monosomic addition line. More pronounced differential staining and size differences of chromosomes were observed in B. campestris than in B. alboglabra. The karyotype of B. campestris was composed of four median (m), four submedian (sm), and two subterminal (st) chromosome pairs, while that of B. alboglabra was composed of three m, four sm, and two st chromosome pairs, provided that the length of the satellite was excluded when determining the arm ratio of the nucleolar chromosome. The alien chromosome from the C genome in the addition line was easily identified in the background B. campestris genome by its large size, its submedian centromere, and its differential staining pattern. When compared with the karyotype of B. alboglabra, the alien chromosome from the C genome in the monosomic addition line was revealed to be chromosome 4.Key words: Brassica campestris, Brassica alboglabra, addition line, mitotic karyotype, gene localization.

Proteomic analysis of sugar beet apomictic monosomic addition line M14

2009 ◽  
Vol 73 (2) ◽  
pp. 297-308 ◽  
Author(s):  
Haiying Li ◽  
Hongxiang Cao ◽  
Yuguang Wang ◽  
Qiuying Pang ◽  
Chunquan Ma ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Proteomic Analysis ◽  
Addition Line ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Salt stress response of membrane proteome of sugar beet monosomic addition line M14

2015 ◽  
Vol 127 ◽  
pp. 18-33 ◽  
Author(s):  
Haiying Li ◽  
Yu Pan ◽  
Yongxue Zhang ◽  
Chuan Wu ◽  
Chunquan Ma ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Sugar Beet ◽  
Addition Line ◽  
Membrane Proteome ◽  
Salt Stress Response ◽  
Monosomic Addition ◽  
Monosomic Addition Line

scholarly journals Cys-SH based quantitative redox proteomics of salt induced response in sugar beet monosomic addition line M14

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Jinna Li ◽  
Kun Wang ◽  
Meichao Ji ◽  
Tingyue Zhang ◽  
Chao Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Double Labeling ◽  
Bisphosphate Carboxylase ◽  
Salt Stress Response ◽  
Redox Proteins ◽  
Ros Homeostasis ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Abstract Background Salt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress. Results In this work, the changes in the BvM14 proteome and redox proteome induced by salt stress were analyzed using a multiplex iodoTMTRAQ double labeling quantitative proteomics approach. A total of 80 proteins were differentially expressed under salt stress. Interestingly, A total of 48 redoxed peptides were identified for 42 potential redox-regulated proteins showed differential redox change under salt stress. A large proportion of the redox proteins were involved in photosynthesis, ROS homeostasis and other pathways. For example, ribulose bisphosphate carboxylase/oxygenase activase changed in its redox state after salt treatments. In addition, three redox proteins involved in regulation of ROS homeostasis were also changed in redox states. Transcription levels of eighteen differential proteins and redox proteins were profiled. (The proteomics data generated in this study have been submitted to the ProteomeXchange and can be accessed via username: [email protected], password: q9YNM1Pe and proteomeXchange# PXD027550.) Conclusions The results showed involvement of protein redox modifications in BvM14 salt stress response and revealed the short-term salt responsive mechanisms. The knowledge may inform marker-based breeding effort of sugar beet and other crops for stress resilience and high yield.

Quantitative proteomics and phosphoproteomics of sugar beet monosomic addition line M14 in response to salt stress

2016 ◽  
Vol 143 ◽  
pp. 286-297 ◽  
Author(s):  
Bing Yu ◽  
Jinna Li ◽  
Jin Koh ◽  
Craig Dufresne ◽  
Na Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Quantitative Proteomics ◽  
Addition Line ◽  
Monosomic Addition ◽  
Monosomic Addition Line

scholarly journals Cys-SH based Quantitative Redox Proteomics of Salt Induced Response in Sugar Beet Monosomic Addition Line M14

2021 ◽  
Author(s):  
Jinna Li ◽  
Meichao Ji ◽  
Tingyue Zhang ◽  
Chao Yang ◽  
He Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Double Labeling ◽  
Bisphosphate Carboxylase ◽  
Salt Stress Response ◽  
Redox Proteins ◽  
Ros Homeostasis ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Abstract Background: Salt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress. Results: In this work, the changes in the BvM14 proteome and redox proteome induced by salt stress were analyzed using a multiplex iodoTMTRAQ double labeling quantitative proteomics approach. A total of 80 proteins were differentially expressed under salt stress. Interestingly, 42 potential redox-regulated proteins showed differential redox change under salt stress. A large proportion of the redox proteins were involved in photosynthesis, ROS homeostasis and other pathways. For example, ribulose bisphosphate carboxylase/oxygenase activase changed in its redox state after salt treatments. In addition, three redox proteins involved in regulation of ROS homeostasis were also changed in redox states. Transcription levels of eighteen differential proteins and redox proteins were profiled. Conclusions: The results showed involvement of protein redox modifications in BvM14 salt stress response and revealed the short-term salt responsive mechanisms. The knowledge may inform marker-based breeding effort of sugar beet and other crops for stress resilience and high yield.

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