scholarly journals Cys-SH based quantitative redox proteomics of salt induced response in sugar beet monosomic addition line M14

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Jinna Li ◽  
Kun Wang ◽  
Meichao Ji ◽  
Tingyue Zhang ◽  
Chao Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Double Labeling ◽  
Bisphosphate Carboxylase ◽  
Salt Stress Response ◽  
Redox Proteins ◽  
Ros Homeostasis ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Abstract Background Salt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress. Results In this work, the changes in the BvM14 proteome and redox proteome induced by salt stress were analyzed using a multiplex iodoTMTRAQ double labeling quantitative proteomics approach. A total of 80 proteins were differentially expressed under salt stress. Interestingly, A total of 48 redoxed peptides were identified for 42 potential redox-regulated proteins showed differential redox change under salt stress. A large proportion of the redox proteins were involved in photosynthesis, ROS homeostasis and other pathways. For example, ribulose bisphosphate carboxylase/oxygenase activase changed in its redox state after salt treatments. In addition, three redox proteins involved in regulation of ROS homeostasis were also changed in redox states. Transcription levels of eighteen differential proteins and redox proteins were profiled. (The proteomics data generated in this study have been submitted to the ProteomeXchange and can be accessed via username: [email protected], password: q9YNM1Pe and proteomeXchange# PXD027550.) Conclusions The results showed involvement of protein redox modifications in BvM14 salt stress response and revealed the short-term salt responsive mechanisms. The knowledge may inform marker-based breeding effort of sugar beet and other crops for stress resilience and high yield.

scholarly journals Cys-SH based Quantitative Redox Proteomics of Salt Induced Response in Sugar Beet Monosomic Addition Line M14

2021 ◽  
Author(s):  
Jinna Li ◽  
Meichao Ji ◽  
Tingyue Zhang ◽  
Chao Yang ◽  
He Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Double Labeling ◽  
Bisphosphate Carboxylase ◽  
Salt Stress Response ◽  
Redox Proteins ◽  
Ros Homeostasis ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Abstract Background: Salt stress is a major abiotic stress that limits plant growth, development and productivity. Studying the molecular mechanisms of salt stress tolerance may help to enhance crop productivity. Sugar beet monosomic addition line M14 exhibits tolerance to salt stress. Results: In this work, the changes in the BvM14 proteome and redox proteome induced by salt stress were analyzed using a multiplex iodoTMTRAQ double labeling quantitative proteomics approach. A total of 80 proteins were differentially expressed under salt stress. Interestingly, 42 potential redox-regulated proteins showed differential redox change under salt stress. A large proportion of the redox proteins were involved in photosynthesis, ROS homeostasis and other pathways. For example, ribulose bisphosphate carboxylase/oxygenase activase changed in its redox state after salt treatments. In addition, three redox proteins involved in regulation of ROS homeostasis were also changed in redox states. Transcription levels of eighteen differential proteins and redox proteins were profiled. Conclusions: The results showed involvement of protein redox modifications in BvM14 salt stress response and revealed the short-term salt responsive mechanisms. The knowledge may inform marker-based breeding effort of sugar beet and other crops for stress resilience and high yield.

Salt stress response of membrane proteome of sugar beet monosomic addition line M14

2015 ◽  
Vol 127 ◽  
pp. 18-33 ◽  
Author(s):  
Haiying Li ◽  
Yu Pan ◽  
Yongxue Zhang ◽  
Chuan Wu ◽  
Chunquan Ma ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Sugar Beet ◽  
Addition Line ◽  
Membrane Proteome ◽  
Salt Stress Response ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Salt stress induced proteome and transcriptome changes in sugar beet monosomic addition line M14

2012 ◽  
Vol 169 (9) ◽  
pp. 839-850 ◽  
Author(s):  
Le Yang ◽  
Chunquan Ma ◽  
Linlin Wang ◽  
Sixue Chen ◽  
Haiying Li
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Addition Line ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Quantitative proteomics and phosphoproteomics of sugar beet monosomic addition line M14 in response to salt stress

2016 ◽  
Vol 143 ◽  
pp. 286-297 ◽  
Author(s):  
Bing Yu ◽  
Jinna Li ◽  
Jin Koh ◽  
Craig Dufresne ◽  
Na Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Quantitative Proteomics ◽  
Addition Line ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Proteomic analysis of sugar beet apomictic monosomic addition line M14

2009 ◽  
Vol 73 (2) ◽  
pp. 297-308 ◽  
Author(s):  
Haiying Li ◽  
Hongxiang Cao ◽  
Yuguang Wang ◽  
Qiuying Pang ◽  
Chunquan Ma ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Proteomic Analysis ◽  
Addition Line ◽  
Monosomic Addition ◽  
Monosomic Addition Line

Quantitative Redox Proteomics Revealed Molecular Mechanisms of Salt Tolerance in the Roots of Sugar Beet Monomeric Addition line M14

2021 ◽  
Author(s):  
He Liu ◽  
Xiaoxue Du ◽  
Jialin Zhang ◽  
Jinna Li ◽  
Sixue Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Salt Stress ◽  
Sugar Beet ◽  
Redox Regulation ◽  
Molecular Mechanisms ◽  
Growth And Yield ◽  
Transcriptional Level ◽  
Addition Line ◽  
Redox Proteomics ◽  
Redox Proteins

Abstract Background: Salt stress is often associated with excessive production of reactive oxygen species (ROS). Oxidative stress caused by the accumulation of ROS is a major factor that negatively affects crop growth and yield. Root is the primary organ that senses and transmits the salt stress signal to the whole plant. How oxidative stress affect redox sensitive proteins in the roots is not known.Results: In this study, the redox proteome of sugar beet M14 roots under salt stress was investigated. Using iTRAQ reporters, we determined that salt stress caused significant changes in the abundance of many proteins (2305 at 20 min salt stress and 2663 at 10 min salt stress). Using iodoTMT reporters, a total of 95 redox proteins were determined to be responsive to salt stress after normalizing again total protein level changes. Notably, most of the differential redox proteins were involved in metabolism, ROS homeostasis, and stress and defense, while a small number play a role in transport, biosynthesis, signal transduction, transcription and photosynthesis. Transcription levels of 14 genes encoding the identified redox proteins were analyzed using qRT-PCR. All the genes were induced by salt stress at the transcriptional level. Conclusions: Based on the redox proteomics results, we construct a map of the regulatory network of M14 root redox proteins in response to salt stress. This study further refines the molecular mechanism of salt resistance at the level of protein redox regulation.

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