Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize MIK

2008 ◽  
Vol 117 (8) ◽  
pp. 1291-1301 ◽  
Author(s):  
S. I. Kim ◽  
C. B. Andaya ◽  
J. W. Newman ◽  
S. S. Goyal ◽  
T. H. Tai
Keyword(s):  
Phytic Acid ◽  
Low Phytic Acid ◽  
Rice Mutant ◽  
Isolation And Characterization

scholarly journals Metabolomic Characterization of a Low Phytic Acid and High Anti-oxidative Cultivar of Turmeric

2015 ◽  
Vol 10 (2) ◽  
pp. 1934578X1501000
Author(s):  
Ken Tanaka ◽  
Masanori Arita ◽  
Donghan Li ◽  
Naoaki Ono ◽  
Yasuhiro Tezuka ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Metal Ion ◽  
Curcuma Longa ◽  
Food Additives ◽  
Chemical Properties ◽  
Lower Amount ◽  
Low Phytic Acid ◽  
History Of ◽  
History Of Use

Turmeric, the rhizome of Curcuma longa, has a long history of use as a spice and also as a traditional medicine in many Asian countries. To reveal unique morphological features of a newly registered Curcuma cultivar, C longa cv. Okinawa Ougon (Ougon), non-targeted LC-MS and GC-MS analyses were conducted. The analysis revealed its distinctive chemical properties: lower amount of phytic acid and inorganic metals such as Fe, Mn, and Al, as well as higher concentrations of reduced derivatives of curcuminoids, such as dihydrobisdemethoxycurcumin, tetrahydrobisdemethoxycurcumin, dihydrodemethoxycurcumin, and tetrahydrodemethoxycurcumin. In addition, germacrane-type sesquiterpenes were almost absent although α-humulene and β-caryophyllene, generated by the same biosynthetic route, were present. Presumably the alternation of the metal ion content, serving as a cofactor of sesquiterpene synthase, modulates the resulting variation of the sesquiterpenes. In summary, the cultivar Ougon is considered a promising candidate for functional food additives.

scholarly journals Characterization of the Symbiotic Nitrogen-Fixing Common Bean Low Phytic Acid (lpa1) Mutant Response to Water Stress

Genes ◽  
2018 ◽  
Vol 9 (2) ◽  
pp. 99 ◽  
Author(s):  
Remo Chiozzotto ◽  
Mario Ramírez ◽  
Chouhra Talbi ◽  
Eleonora Cominelli ◽  
Lourdes Girard ◽  
...  
Keyword(s):  
Water Stress ◽  
Common Bean ◽  
Phytic Acid ◽  
Nitrogen Fixing ◽  
Low Phytic Acid ◽  
Response To Water

scholarly journals Phytic Acid and Transporters: What Can We Learn from low phytic acid Mutants?

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 69 ◽  
Author(s):  
Eleonora Cominelli ◽  
Roberto Pilu ◽  
Francesca Sparvoli
Keyword(s):  
Phytic Acid ◽  
Seed Quality ◽  
Sulfur Metabolism ◽  
In Silico Analysis ◽  
Point Of View ◽  
Low Phytic Acid ◽  
Cellular Processes ◽  
Report Data ◽  
Silico Analysis

Phytic acid has two main roles in plant tissues: Storage of phosphorus and regulation of different cellular processes. From a nutritional point of view, it is considered an antinutritional compound because, being a cation chelator, its presence reduces mineral bioavailability from the diet. In recent decades, the development of low phytic acid (lpa) mutants has been an important goal for nutritional seed quality improvement, mainly in cereals and legumes. Different lpa mutations affect phytic acid biosynthetic genes. However, other lpa mutations isolated so far, affect genes coding for three classes of transporters: A specific group of ABCC type vacuolar transporters, putative sulfate transporters, and phosphate transporters. In the present review, we summarize advances in the characterization of these transporters in cereals and legumes. Particularly, we describe genes, proteins, and mutants for these different transporters, and we report data of in silico analysis aimed at identifying the putative orthologs in some other cereal and legume species. Finally, we comment on the advantage of using such types of mutants for crop biofortification and on their possible utility to unravel links between phosphorus and sulfur metabolism (phosphate and sulfate homeostasis crosstalk).

scholarly journals The nuclear-localized PPR protein OsNPPR1 is important for mitochondrial function and endosperm development in rice

2019 ◽  
Vol 70 (18) ◽  
pp. 4705-4720 ◽  
Author(s):  
Yuanyuan Hao ◽  
Yunlong Wang ◽  
Mingming Wu ◽  
Xiaopin Zhu ◽  
Xuan Teng ◽  
...  
Keyword(s):  
Mitochondrial Function ◽  
Endosperm Development ◽  
Pentatricopeptide Repeat ◽  
Coding Region ◽  
Rice Mutant ◽  
Ppr Protein ◽  
Isolation And Characterization ◽  
Ppr Proteins

Abstract Pentatricopeptide repeat (PPR) proteins constitute one of the largest protein families in land plants. Recent studies revealed the functions of PPR proteins in organellar RNA metabolism and plant development, but the functions of most PPR proteins, especially PPRs localized in the nucleus, remain largely unknown. Here, we report the isolation and characterization of a rice mutant named floury and growth retardation1 (fgr1). fgr1 showed floury endosperm with loosely arranged starch grains, decreased starch and amylose contents, and retarded seedling growth. Map-based cloning showed that the mutant phenotype was caused by a single nucleotide substitution in the coding region of Os08g0290000. This gene encodes a nuclear-localized PPR protein, which we named OsNPPR1, that affected mitochondrial function. In vitro SELEX and RNA-EMSAs showed that OsNPPR1 was an RNA protein that bound to the CUCAC motif. Moreover, a number of retained intron (RI) events were detected in fgr1. Thus, OsNPPR1 was involved in regulation of mitochondrial development and/or functions that are important for endosperm development. Our results provide novel insights into coordinated interaction between nuclear-localized PPR proteins and mitochondrial function.

Identification and Characterization of a Low Phytic Acid Wheat

Crop Science ◽  
2004 ◽  
Vol 44 (2) ◽  
pp. 418 ◽  
Author(s):  
Mary Guttieri ◽  
David Bowen ◽  
John A. Dorsch ◽  
Victor Raboy ◽  
Edward Souza
Keyword(s):  
Phytic Acid ◽  
Low Phytic Acid ◽  
Identification And Characterization

Identification and Characterization of a Low Phytic Acid Wheat

Crop Science ◽  
2004 ◽  
Vol 44 (2) ◽  
pp. 418-424 ◽  
Author(s):  
Mary Guttieri ◽  
David Bowen ◽  
John A. Dorsch ◽  
Victor Raboy ◽  
Edward Souza
Keyword(s):  
Phytic Acid ◽  
Low Phytic Acid ◽  
Identification And Characterization

Identification and Characterization of a Low Phytic Acid Wheat

Crop Science ◽  
2004 ◽  
Vol 44 (4) ◽  
pp. 1505-1505 ◽  
Author(s):  
Mary Guttieri ◽  
David Bowen ◽  
John A. Dorsch ◽  
Victor Raboy ◽  
Edward Souza
Keyword(s):  
Phytic Acid ◽  
Low Phytic Acid ◽  
Identification And Characterization

Impact of cross-breeding on the metabolites of the low phytic acid rice mutant Os-lpa-MH86-1.

2021 ◽  
pp. 433-443
Author(s):  
Chen-guang Zhou ◽  
Yuan-yuan Tan ◽  
Sophia Gossner ◽  
You-fa Li ◽  
Qing-yao Shu ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Seed Viability ◽  
Mutation Breeding ◽  
Cross Breeding ◽  
Low Phytic Acid ◽  
Rice Mutant ◽  
Transporter Family ◽  
Independent Field ◽  
Food And Feed ◽  
The Stability

Abstract Phytic acid (myo-inositol-1,2,3,4,5,6-hexakisphosphate), the major storage form of phosphorus in cereals, is considered as an antinutrient in food and feed. During the past few years, various cereals have been subjected to mutation breeding for generating low phytic acid (lpa) crops. Recently, it was demonstrated that reduction of phytic acid in the rice mutant Os-lpa-MH86-1 obtained by gamma irradiation was due to a disruption of OsSULTR3;3, an orthologue of the sulfate transporter family group 3 genes. The application of a GC/MS-based metabolite profiling approach revealed that the reduction of phytic acid was accompanied by changes in concentrations of metabolites from different classes in the Os-lpa-MH86-1 mutant.Lpa mutant lines often exhibit lower grain yield and seed viability compared with their wild-type parents. To improve the agronomic performance of the Os-lpa-MH86-1 mutant, cross-breeding with a commercial cultivar was performed. The resulting progenies were genotyped using molecular markers to identify homozygous wildtype and lpa mutants from generations F4 to F7. The objectives of this study were: (i) to observe the consistent metabolic changes in Os-lpa-MH86-1 lpa mutants by following their composition over several independent field trials; (ii) to investigate the impact of cross-breeding on the phytic acid content and the metabolic phenotype of the homozygous lpa mutant; and (iii) to assess the stability of the mutation-specific metabolite signature in the lpa progenies over several generations. Statistical assessment of the data via multivariate and univariate approaches demonstrated that the lpa trait and the mutation-induced metabolite signature in the lpa progenies were comparable to the progenitor Os-lpa-MH86-1 mutant and consistently expressed over generations. These findings extend the basis for implementing mutation breeding in the generation of lpa rice cultivars.

scholarly journals Isolation and Characterization of a Rice Mutant Hypersensitive to Al

2005 ◽  
Vol 46 (7) ◽  
pp. 1054-1061 ◽  
Author(s):  
Jian Feng Ma ◽  
Sakiko Nagao ◽  
Chao Feng Huang ◽  
Minoru Nishimura
Keyword(s):  
Rice Mutant ◽  
Isolation And Characterization
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