Molecular markers from a BAC contig spanning the Rdr1 locus: a tool for marker-assisted selection in roses

Abstract Experiments were conducted to validate different molecular markers associated with sugar beet breeding using available sugar beet monogerm germplasm resources to explore their effectiveness in different sugar beet populations. We used multiple primer pairs to amplify sugar beet monogerm sterile and maintainer lines in order to verify their polymorphism. For the nucleus Rf1 locus genotype enzyme digestion was also required to verify. The results showed that three pairs of primers, TR1, s17 and 11E8M4S, produced polymorphism when amplifying sugar beet sterile and maintainer lines; primers o7 and AB-18, although polymorphic, did not correlate significantly with sugar beet fertility; primer cpSSR-2 did not produce significant band differences when amplifying sterile and maintainer lines, however, the number of single nucleotide sequence repeats of base A needed to be further verified as a basis for differentiating sugar beet fertility. The polymorphism of 15 pairs of primers related to sugar beet fertility identification was verified and TR1, s17 and 11E8M4S could be used to differentiate sugar beet sterile lines from the maintainer lines and used for subsequent Marker-Assisted Selection (MAS).

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Application Techniques of Molecular Marker and Achievement of Marker Assisted Selection (MAS) in Three Major Crops Rice, Wheat and Maize

International Journal for Research in Applied Sciences and Biotechnology ◽

10.31033/ijrasb.8.1.10 ◽

2021 ◽

Vol 8 (1) ◽

pp. 82-93

Author(s):

Muhammad Gul Arabzai ◽

Hameed Gul

Keyword(s):

Molecular Markers ◽

Molecular Marker ◽

Abiotic Stresses ◽

Marker Assisted Selection ◽

Agronomic Traits ◽

Linkage Drag ◽

Conventional Breeding ◽

Phenotype Data ◽

Application Techniques ◽

New Varieties

With the discovery of new genetic technology, the researcher focuses on using DNA molecular markers to improve new varieties worldwide. Such as resistance to biotic and abiotic stresses and enhancing quality and quantity at different plant breeding fields. Conventional breeding selection is based on phenotype data selection, time-consuming, and has a high chance of linkage drag. Thus, DNA molecular marker method usage is faster, easy, and not expensive than conventional breeding programs. This review focused on applying molecular markers such as genetic diversity analysis, the genotype of identification and fingerprinting, gene tagging and mapping, QTL analysis, and marker-assisted selection. In another part of this review, we focused on MAS's achievements related to improving agronomic traits, quality traits, and biotic/abiotic stresses for three major cereal crops like Wheat, Rice, and Maize.

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Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/27/3110 ◽

2007 ◽

pp. 100-104

Author(s):

Stella Molnár ◽

Zsuzsanna Galbács ◽

Gábor Halász ◽

Sarolta Hoffmann ◽

Anikó Veres ◽

...

Keyword(s):

Molecular Markers ◽

Powdery Mildew ◽

Marker Assisted Selection ◽

Powdery Mildew Resistance ◽

Resistance Breeding ◽

Breeding Programs ◽

Pcr Rflp ◽

Muscadinia Rotundifolia ◽

Selection Of ◽

Grape Breeding

Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

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Development of SNP Set for the Marker-Assisted Selection of Guar (Cyamopsis tetragonoloba (L.) Taub.) Based on a Custom Reference Genome Assembly

Plants ◽

10.3390/plants10102063 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2063

Author(s):

Elizaveta Grigoreva ◽

Yury Barbitoff ◽

Anton Changalidi ◽

Dmitry Karzhaev ◽

Vladimir Volkov ◽

...

Keyword(s):

Molecular Markers ◽

Marker Assisted Selection ◽

Genome Wide Association Study ◽

Reference Genome ◽

Guar Gum ◽

Inositol Phosphates ◽

Inositol Phosphate ◽

Industrial Applications ◽

Gas Production ◽

Cyamopsis Tetragonoloba

Guar gum, a polysaccharide derived from guar seeds, is widely used in a variety of industrial applications, including oil and gas production. Although guar is mostly propagated in India, interest in guar as a new industrial legume crop is increasing worldwide, demanding the development of effective tools for marker-assisted selection. In this paper, we report a wide-ranging set of 4907 common SNPs and 327 InDels generated from RADseq genotyping data of 166 guar plants of different geographical origin. A custom guar reference genome was assembled and used for variant calling. A consensus set of variants was built using three bioinformatic pipelines for short variant discovery. The developed molecular markers were used for genome-wide association study, resulting in the discovery of six markers linked to the variation of an important agronomic trait—percentage of pods matured to the harvest date under long light day conditions. One of the associated variants was found inside the putative transcript sequence homologous to an ABC transporter in Arabidopsis, which has been shown to play an important role in D-myo-inositol phosphates metabolism. Earlier, we suggested that genes involved in myo-inositol phosphate metabolism have significant impact on the early flowering of guar plants. Hence, we believe that the developed SNP set allows for the identification of confident molecular markers of important agrobiological traits.

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