BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo candida

White rust (Albugo candida) is a highly destructive disease of oilseed Brassicas such as Brassica juncea and B. rapa. Most commercial B. juncea or B. rapa varieties are highly susceptible and yield losses from combined infection of leaves and inflorescences can be up to 20% or 60% in Australia and India, respectively. In Australia, canola-quality B. juncea has been developed to extend oilseed Brassica production into lower rainfall areas, with the first commercial B. juncea canola-quality variety planned for release in 2006. It is essential to identify useful sources of host resistance in B. juncea as breeding and/or selection of material for resistance is the most cost-effective method of delivering control for farmers. Three experiments were undertaken under controlled-environmental conditions to identify the best methods of characterising host resistance and to identify sources of resistance in B. juncea germplasm from Australia, China, and India. Forty-four B. juncea genotypes, viz. 22 from India, 12 from Australia, and 10 from China, were tested. Four Chinese genotypes (CBJ-001, CBJ-002, CBJ-003, CBJ-004) and one Australian genotype (JR049) consistently showed high resistance to A. candida across the different plant growth stages against a pathotype prevailing in Australia. Similarly, the most susceptible genotypes (viz. Indian genotypes RH781, RL1359, RH819) were extremely susceptible irrespective of the plant growth stage. Overall, although disease severity on cotyledons and leaves at the different growth stages was significantly and positively correlated, there was, however, no significant correlation between the number of stagheads and any of the other disease parameters measured. Our study demonstrates that controlled-environmental conditions are suitable for rapid identification of resistant genotypes and that genotypes with high levels of resistance can be reliably identified at the cotyledonary, seedling, or flowering stages.

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INHERITANCE OF RESISTANCE TO Albugo Candida Race 2 IN MUSTARD (Brassica Juncea (L.) Czern.)

Canadian Journal of Plant Science ◽

10.4141/cjps88-039 ◽

1988 ◽

Vol 68 (2) ◽

pp. 297-300 ◽

Cited By ~ 20

Author(s):

A. S. TIWARI ◽

G. A. PETRIE ◽

R. K. DOWNEY

Keyword(s):

Brassica Juncea ◽

Nuclear Genes ◽

Growth Chamber ◽

Inheritance Of Resistance ◽

Resistant Parent ◽

Albugo Candida ◽

White Rust ◽

Race 2 ◽

Made In

The inheritance of resistance to white rust (Albugo Candida) race 2 in mustard (Brassica juncea) was studied in crosses involving one resistant and two susceptible cultivars. Inoculations were made in a growth chamber followed by growth of the plants under greenhouse conditions. The reaction of the F1 was like the resistant parent, indicating that resistance is dominant and controlled by nuclear genes. Backcrosses of F1 plants to the resistant parent showed the same reactions as that of the resistant parent. Backcrosses of F1 to the susceptible parents segregated in a 1:1 ratio of resistant to susceptible. The F2 segregation of resistant and susceptible plants gave a good fit to a 3:1 ratio. The study revealed that resistance is monogenic and could be easily transferred to adapted susceptible genotypes via backcrossing.Key words: Brassica juncea, Albugo Candida, mustard, white rust

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Study of White Rust (Albugo candida) Management Approach in Indian Mustard (Brassica juncea) at Farmers Fields through on farm Testing (OFT) at Farmers Fields

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2020.910.451 ◽

2020 ◽

Vol 9 (10) ◽

pp. 3916-3921

Author(s):

Sunil Kumar ◽

Sushil Kumar Sharma ◽

Pankaj Sharma ◽

P. K. Rai

Keyword(s):

Brassica Juncea ◽

Indian Mustard ◽

Management Approach ◽

Albugo Candida ◽

White Rust ◽

On Farm

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Valuable New Leaf or Inflorescence Resistances Ensure Improved Management of White Rust (Albugo candida ) in Mustard (Brassica juncea ) Crops

Journal of Phytopathology ◽

10.1111/jph.12425 ◽

2015 ◽

Vol 164 (6) ◽

pp. 404-411 ◽

Cited By ~ 2

Author(s):

Martin J. Barbetti ◽

Cai Xia Li ◽

Ming Pei You ◽

Dhiraj Singh ◽

Abha Agnihotri ◽

...

Keyword(s):

Brassica Juncea ◽

Albugo Candida ◽

White Rust

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SCIMITAR BROWN MUSTARD

Canadian Journal of Plant Science ◽

10.4141/cjps89-031 ◽

1989 ◽

Vol 69 (1) ◽

pp. 247-248 ◽

Cited By ~ 2

Author(s):

D. L. WOODS ◽

G. A. PETRIE

Keyword(s):

Key Words ◽

Brassica Juncea ◽

Western Canada ◽

Albugo Candida ◽

White Rust ◽

Cultivar Description

Scimitar brown mustard (Brassica juncea (L.) Coss), registered in 1987, is a totally brown-seeded cultivar which combines a high glucosinolate level with resistance to white rust (Albugo Candida (Pers. ex Lev.) Ktze.). It is adapted to all areas in western Canada where B. juncea is grown, but is later maturing than other commonly grown cultivars (Blaze, Commercial brown).Key words: Mustard (brown), cultivar description

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Acantozero erucic acid oriental condiment mustard

Canadian Journal of Plant Science ◽

10.4141/cjps09155 ◽

2010 ◽

Vol 90 (4) ◽

pp. 499-502

Author(s):

G. Rakow ◽

J P Raney ◽

D. Rode

Keyword(s):

Erucic Acid ◽

Brassica Juncea ◽

Seed Oil ◽

Seed Protein ◽

Leptosphaeria Maculans ◽

Glucosinolate Content ◽

Blackleg Disease ◽

Albugo Candida ◽

White Rust ◽

Cultivar Description

Acanto is the first zero erucic acid oriental condiment mustard [Brassica juncea (L.) Czern.]. It has the same grain yield, plant height, seed protein content, seed weight, allyl glucosinolate content, and seed chlorophyll content as the check cultivar Cutlass. Both cultivars are highly resistant to blackleg disease caused by Leptosphaeria maculans (Desm.) Ces et de Not., but are susceptible to white rust race 2v caused by Albugo candida (Pers.) Kuntze. Acanto is more susceptible to white rust race 2a than is Cutlass. Acanto matures 2 d later than Cutlass, has a 2.9% lower seed oil (fixed oil) content and its seed colour is a darker yellow. Acanto was well adapted to the mustard growing areas of the Canadian prairies.Key words: Brassica juncea (L.) Czern., cultivar description, zero erucic acid oil

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BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo Candida

10.1101/509851 ◽

2019 ◽

Author(s):

Heena Arora ◽

K. Lakshmi Padmaja ◽

Kumar Paritosh ◽

Nitika Mukhi ◽

A. K. Tewari ◽

...

Keyword(s):

Genetic Transformation ◽

Brassica Juncea ◽

Gene Pool ◽

Evolutionary Dynamics ◽

Genomic Sequence ◽

Oilseed Crop ◽

R Gene ◽

East European ◽

Albugo Candida ◽

White Rust

AbstractWhite rust caused by oomycete pathogen Albugo candida is a significant disease of crucifer crops including Brassica juncea (mustard), a major oilseed crop of the Indian subcontinent. Earlier a resistance-conferring locus named AcB1-A5.1 was mapped in an east European gene pool line of B. juncea – Donskaja-IV. This line was tested along with some other lines of B. juncea (AABB), B. rapa (AA) and B. nigra (BB) for resistance to six isolates of A. candida collected from different mustard growing regions of India. Donskaja-IV was found to be completely resistant to all the tested isolates. Sequencing of a BAC spanning the locus AcB1-A5.1 showed the presence of a single CC-NB-LRR protein encoding R gene. The genomic sequence of the putative R gene with its native promoter and terminator was used for the genetic transformation of a susceptible Indian gene pool line Varuna and was found to confer complete resistance to all the isolates. This is the first white rust resistance-conferring gene described from Brassica species and has been named BjuWRR1. Allelic variants of the gene in B. juncea germplasm and orthologues in the Brassicaceae genomes were studied to understand the evolutionary dynamics of the BjuWRR1 gene.HighlightBjuWRR1, a CNL type R gene, was identified from an east European gene pool line of Brassica juncea and validated for conferring resistance to white rust by genetic transformation.

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Identification of RFLP markers linked to the white rust resistance gene (Acr) in mustard (Brassica juncea (L.) Czern. and Coss.)

Genome ◽

10.1139/g98-043 ◽

1998 ◽

Vol 41 (4) ◽

pp. 626-628 ◽

Cited By ~ 16

Author(s):

W Y Cheung ◽

R K Gugel ◽

B S Landry

Keyword(s):

Brassica Juncea ◽

Rust Resistance ◽

Single Gene ◽

Rapid Identification ◽

Rflp Markers ◽

Rust Disease ◽

Albugo Candida ◽

White Rust ◽

Rflp Map ◽

Race 2

White rust and staghead, caused by Albugo candida, is an economically important disease of Brassica juncea and Brassica rapa crops in western Canada. The identification of genes for white rust resistance in these crops and the development of molecular markers for these genes will allow the rapid identification of resistant germplasm and should accelerate the development of white rust resistant cultivars. In this study, 119 F1-derived doubled-haploid progeny lines of a cross between white rust susceptible (J90-4317) and white rust resistant (J90-2733) B. juncea lines were evaluated for resistance to A. candida race 2. A single gene (Acr) responsible for conferring resistance to this pathogen was mapped on a densely populated B. juncea RFLP map developed earlier. A cosegregating RFLP marker (X140a) and two other closely linked RFLP markers (X42 and X83) were identified; the latter two markers were 2.3 and 4 cM from the Acr locus, respectively. These markers may be useful for marker-assisted selection and map-based cloning of this gene.Key words: Brassica juncea, mustard, Albugo candida, white rust, disease resistance, RFLP.

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