scholarly journals BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo Candida

2019 ◽  
Author(s):  
Heena Arora ◽  
K. Lakshmi Padmaja ◽  
Kumar Paritosh ◽  
Nitika Mukhi ◽  
A. K. Tewari ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Brassica Juncea ◽  
Gene Pool ◽  
Evolutionary Dynamics ◽  
Genomic Sequence ◽  
Oilseed Crop ◽  
R Gene ◽  
East European ◽  
Albugo Candida ◽  
White Rust

AbstractWhite rust caused by oomycete pathogen Albugo candida is a significant disease of crucifer crops including Brassica juncea (mustard), a major oilseed crop of the Indian subcontinent. Earlier a resistance-conferring locus named AcB1-A5.1 was mapped in an east European gene pool line of B. juncea – Donskaja-IV. This line was tested along with some other lines of B. juncea (AABB), B. rapa (AA) and B. nigra (BB) for resistance to six isolates of A. candida collected from different mustard growing regions of India. Donskaja-IV was found to be completely resistant to all the tested isolates. Sequencing of a BAC spanning the locus AcB1-A5.1 showed the presence of a single CC-NB-LRR protein encoding R gene. The genomic sequence of the putative R gene with its native promoter and terminator was used for the genetic transformation of a susceptible Indian gene pool line Varuna and was found to confer complete resistance to all the isolates. This is the first white rust resistance-conferring gene described from Brassica species and has been named BjuWRR1. Allelic variants of the gene in B. juncea germplasm and orthologues in the Brassicaceae genomes were studied to understand the evolutionary dynamics of the BjuWRR1 gene.HighlightBjuWRR1, a CNL type R gene, was identified from an east European gene pool line of Brassica juncea and validated for conferring resistance to white rust by genetic transformation.

Expression and relationships of resistance to white rust (Albugo candida) at cotyledonary, seedling, and flowering stages in Brassica juncea germplasm from Australia, China, and India

2007 ◽  
Vol 58 (3) ◽  
pp. 259 ◽  
Author(s):  
C. X. Li ◽  
K. Sivasithamparam ◽  
G. Walton ◽  
P. Salisbury ◽  
W. Burton ◽  
...  
Keyword(s):  
Plant Growth ◽  
Brassica Juncea ◽  
Environmental Conditions ◽  
Host Resistance ◽  
Growth Stages ◽  
Sources Of Resistance ◽  
Albugo Candida ◽  
White Rust ◽  
China And India ◽  
Canola Quality

White rust (Albugo candida) is a highly destructive disease of oilseed Brassicas such as Brassica juncea and B. rapa. Most commercial B. juncea or B. rapa varieties are highly susceptible and yield losses from combined infection of leaves and inflorescences can be up to 20% or 60% in Australia and India, respectively. In Australia, canola-quality B. juncea has been developed to extend oilseed Brassica production into lower rainfall areas, with the first commercial B. juncea canola-quality variety planned for release in 2006. It is essential to identify useful sources of host resistance in B. juncea as breeding and/or selection of material for resistance is the most cost-effective method of delivering control for farmers. Three experiments were undertaken under controlled-environmental conditions to identify the best methods of characterising host resistance and to identify sources of resistance in B. juncea germplasm from Australia, China, and India. Forty-four B. juncea genotypes, viz. 22 from India, 12 from Australia, and 10 from China, were tested. Four Chinese genotypes (CBJ-001, CBJ-002, CBJ-003, CBJ-004) and one Australian genotype (JR049) consistently showed high resistance to A. candida across the different plant growth stages against a pathotype prevailing in Australia. Similarly, the most susceptible genotypes (viz. Indian genotypes RH781, RL1359, RH819) were extremely susceptible irrespective of the plant growth stage. Overall, although disease severity on cotyledons and leaves at the different growth stages was significantly and positively correlated, there was, however, no significant correlation between the number of stagheads and any of the other disease parameters measured. Our study demonstrates that controlled-environmental conditions are suitable for rapid identification of resistant genotypes and that genotypes with high levels of resistance can be reliably identified at the cotyledonary, seedling, or flowering stages.

INHERITANCE OF RESISTANCE TO Albugo Candida Race 2 IN MUSTARD (Brassica Juncea (L.) Czern.)

1988 ◽  
Vol 68 (2) ◽  
pp. 297-300 ◽  
Author(s):  
A. S. TIWARI ◽  
G. A. PETRIE ◽  
R. K. DOWNEY
Keyword(s):  
Brassica Juncea ◽  
Nuclear Genes ◽  
Growth Chamber ◽  
Inheritance Of Resistance ◽  
Resistant Parent ◽  
Albugo Candida ◽  
White Rust ◽  
Race 2 ◽  
Made In

The inheritance of resistance to white rust (Albugo Candida) race 2 in mustard (Brassica juncea) was studied in crosses involving one resistant and two susceptible cultivars. Inoculations were made in a growth chamber followed by growth of the plants under greenhouse conditions. The reaction of the F1 was like the resistant parent, indicating that resistance is dominant and controlled by nuclear genes. Backcrosses of F1 plants to the resistant parent showed the same reactions as that of the resistant parent. Backcrosses of F1 to the susceptible parents segregated in a 1:1 ratio of resistant to susceptible. The F2 segregation of resistant and susceptible plants gave a good fit to a 3:1 ratio. The study revealed that resistance is monogenic and could be easily transferred to adapted susceptible genotypes via backcrossing.Key words: Brassica juncea, Albugo Candida, mustard, white rust

scholarly journals BjuWRR1, a CC-NB-LRR gene identified in Brassica juncea, confers resistance to white rust caused by Albugo candida

2019 ◽  
Vol 132 (8) ◽  
pp. 2223-2236 ◽  
Author(s):  
Heena Arora ◽  
K. Lakshmi Padmaja ◽  
Kumar Paritosh ◽  
Nitika Mukhi ◽  
A. K. Tewari ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Albugo Candida ◽  
White Rust

scholarly journals WRR4 Encodes a TIR-NB-LRR Protein That Confers Broad-Spectrum White Rust Resistance in Arabidopsis thaliana to Four Physiological Races of Albugo candida

2008 ◽  
Vol 21 (6) ◽  
pp. 757-768 ◽  
Author(s):  
M. Hossein Borhan ◽  
Nick Gunn ◽  
Abigail Cooper ◽  
Sigrun Gulden ◽  
Mahmut Tör ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Broad Spectrum ◽  
Rust Resistance ◽  
Functional Expression ◽  
Oilseed Crop ◽  
Transgenic Expression ◽  
Albugo Candida ◽  
Physiological Races ◽  
White Rust ◽  
Race 4

White blister rust in the Brassicaceae is emerging as a superb model for exploring how plant biodiversity has channeled speciation of biotrophic parasites. The causal agents of white rust across a wide breadth of cruciferous hosts currently are named as variants of a single oomycete species, Albugo candida. The most notable examples include a major group of physiological races that each are economically destructive in a different vegetable or oilseed crop of Brassica juncea (A. candida race 2), B. rapa (race 7), or B. oleracea (race 9); or parasitic on wild crucifers such as Capsella bursa-pastoris (race 4). Arabidopsis thaliana is innately immune to these races of A. candida under natural conditions; however, it commonly hosts its own molecularly distinct subspecies of A. candida (A. candida subsp. arabidopsis). In the laboratory, we have identified several accessions of Arabidopsis thaliana (e.g.,. Ws-3) that can permit varying degrees of rust development following inoculation with A. candida races 2, 4, and 7, whereas race 9 is universally incompatible in Arabidopsis thaliana and nonrusting resistance is the most prevalent outcome of interactions with the other races. Subtle variation in resistance phenotypes is evident, observed initially with an isolate of A. candida race 4, indicating additional genetic variation. Therefore, we used the race 4 isolate for map-based cloning of the first of many expected white rust resistance (WRR) genes. This gene was designated WRR4 and encodes a cytoplasmic toll-interleukin receptor-like nucleotide-binding leucine-rich repeat receptor-like protein that confers a dominant, broad-spectrum white rust resistance in the Arabidopsis thaliana accession Columbia to representative isolates of A. candida races 2, 4, 7, and 9, as verified by transgenic expression of the Columbia allele in Ws-3. The WRR4 protein requires functional expression of the lipase-like protein EDS1 but not the paralogous protein PAD4, and confers full immunity that masks an underlying nonhypersensitive incompatibility in Columbia to A. candida race 4. This residual incompatibility is independent of functional EDS1.

scholarly journals A Mapped Locus on LG A6 of Brassica juncea Line Tumida Conferring Resistance to White Rust Contains a CNL Type R Gene

2020 ◽  
Vol 10 ◽  
Author(s):  
Latika Bhayana ◽  
Kumar Paritosh ◽  
Heena Arora ◽  
Satish Kumar Yadava ◽  
Priyansha Singh ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
R Gene ◽  
White Rust

Valuable New Leaf or Inflorescence Resistances Ensure Improved Management of White Rust (Albugo candida ) in Mustard (Brassica juncea ) Crops

2015 ◽  
Vol 164 (6) ◽  
pp. 404-411 ◽  
Author(s):  
Martin J. Barbetti ◽  
Cai Xia Li ◽  
Ming Pei You ◽  
Dhiraj Singh ◽  
Abha Agnihotri ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Albugo Candida ◽  
White Rust

SCIMITAR BROWN MUSTARD

1989 ◽  
Vol 69 (1) ◽  
pp. 247-248 ◽  
Author(s):  
D. L. WOODS ◽  
G. A. PETRIE
Keyword(s):  
Key Words ◽  
Brassica Juncea ◽  
Western Canada ◽  
Albugo Candida ◽  
White Rust ◽  
Cultivar Description

Scimitar brown mustard (Brassica juncea (L.) Coss), registered in 1987, is a totally brown-seeded cultivar which combines a high glucosinolate level with resistance to white rust (Albugo Candida (Pers. ex Lev.) Ktze.). It is adapted to all areas in western Canada where B. juncea is grown, but is later maturing than other commonly grown cultivars (Blaze, Commercial brown).Key words: Mustard (brown), cultivar description

Acantozero erucic acid oriental condiment mustard

2010 ◽  
Vol 90 (4) ◽  
pp. 499-502
Author(s):  
G. Rakow ◽  
J P Raney ◽  
D. Rode
Keyword(s):  
Erucic Acid ◽  
Brassica Juncea ◽  
Seed Oil ◽  
Seed Protein ◽  
Leptosphaeria Maculans ◽  
Glucosinolate Content ◽  
Blackleg Disease ◽  
Albugo Candida ◽  
White Rust ◽  
Cultivar Description

Acanto is the first zero erucic acid oriental condiment mustard [Brassica juncea (L.) Czern.]. It has the same grain yield, plant height, seed protein content, seed weight, allyl glucosinolate content, and seed chlorophyll content as the check cultivar Cutlass. Both cultivars are highly resistant to blackleg disease caused by Leptosphaeria maculans (Desm.) Ces et de Not., but are susceptible to white rust race 2v caused by Albugo candida (Pers.) Kuntze. Acanto is more susceptible to white rust race 2a than is Cutlass. Acanto matures 2 d later than Cutlass, has a 2.9% lower seed oil (fixed oil) content and its seed colour is a darker yellow. Acanto was well adapted to the mustard growing areas of the Canadian prairies.Key words: Brassica juncea (L.) Czern., cultivar description, zero erucic acid oil

QTL mapping of yield-associated traits in Brassica juncea: meta-analysis and epistatic interactions using two different crosses between east European and Indian gene pool lines

2012 ◽  
Vol 125 (7) ◽  
pp. 1553-1564 ◽  
Author(s):  
Satish Kumar Yadava ◽  
N. Arumugam ◽  
Arundhati Mukhopadhyay ◽  
Yashpal Singh Sodhi ◽  
Vibha Gupta ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Brassica Juncea ◽  
Gene Pool ◽  
Meta Analysis ◽  
Epistatic Interactions ◽  
East European
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