Characterization of spelt (Triticum spelta L.) forms by gel-electrophoretic analyses of seed storage proteins. III. Comparative analyses of spelt and Central European winter wheat (Triticum aestivum L.) cultivars by SDS-PAGE and acid-PAGE

1998 ◽  
Vol 97 (8) ◽  
pp. 1340-1346 ◽  
Author(s):  
H. Radić-Miehle ◽  
C. Saam ◽  
R. Hüls ◽  
C. I. Kling ◽  
C. U. Hesemann
Keyword(s):  
Triticum Aestivum ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Triticum Spelta ◽  
Central European ◽  
Sds Page ◽  
European Winter Wheat

Characterization of spelt (Triticum spelta L.) forms by gel electrophoretic analyses of seed storage proteins. I. The gliadins

1997 ◽  
Vol 94 (1) ◽  
pp. 52-60 ◽  
Author(s):  
S. Harsch ◽  
T. Günther ◽  
B. Rozynek ◽  
C. U. Hesemann ◽  
Ch. I. Kling
Keyword(s):  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Spelta

Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

2011 ◽  
Vol 9 (2) ◽  
pp. 256-259
Author(s):  
Zuzana Šramková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Ernest Šturdík
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Polyacrylamide Gel

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

Homology of chromosomes of Triticum macha Dek. et Men. and Triticum aestivum L. as shown with the help of genetic markers

Genome ◽  
1990 ◽  
Vol 33 (5) ◽  
pp. 755-757 ◽  
Author(s):  
E. V. Metakovsky ◽  
Z. A. Iakobashvili
Keyword(s):  
Triticum Aestivum ◽  
Genetic Analysis ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Close Relationship ◽  
Homology Relationship ◽  
Relationship Of ◽  
Triticum Macha

Inheritance of the storage protein (gliadin and glutenin) genes of Triticum macha Dek. et Men. and their allelism to Triticum aestivum L. genes have been studied. A close homology of at least chromosomes 1A and 1B of the two species has been found. Results confirm a very close relationship between T. macha and T. aestivum.Key words: seed storage proteins, genetic analysis, chromosome homology, relationship of Triticum macha Dek. et Men. and Triticum aestivum L.

scholarly journals Characterization of Tunisian castor bean genotypes using SDS-PAGE of total seed storage proteins

10.5219/973 ◽  
2018 ◽  
Vol 12 (1) ◽  
Author(s):  
Martin Vivodík ◽  
Ezzeddine Saadaoui ◽  
Zdenka Gálová ◽  
Želmíra Balážová ◽  
Lenka Petrovičová
Keyword(s):  
Castor Bean ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Sds Page ◽  
Total Seed

scholarly journals Isolation and properties of a metalloproteinase from buckwheat (Fagopyrum esculentum) seeds

1990 ◽  
Vol 272 (3) ◽  
pp. 677-682 ◽  
Author(s):  
M A Belozersky ◽  
Y E Dunaevsky ◽  
N E Voskoboynikova
Keyword(s):  
Storage Proteins ◽  
Limited Proteolysis ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Fagopyrum Esculentum ◽  
Gel Chromatography ◽  
Ph Optimum ◽  
Peptide Bonds ◽  
Sds Page ◽  
Homogeneous Preparation

A homogeneous preparation of metalloproteinase, purified 1000-fold, was obtained from buckwheat (Fagopyrum esculentum) seeds. The Mr of the enzyme, determined by SDS/PAGE, was 34,000 (it was 39,000 by gel chromatography). Its pH optimum was 8.0-8.2 with 13 S globulin, from buckwheat seeds, as substrate. Atomic-absorption spectroscopy revealed the presence of one Zn2+ ion per enzyme molecule. The enzyme was completely inhibited by EDTA (1 mM), zincone (1 mM) and 1, 10-phenanthroline (1 mM). The metalloproteinase performed limited proteolysis of the following seed storage proteins: 13 S globulin from buckwheat seeds and 11 S globulin from soybean (Glycine max) seeds. It hydrolysed three peptide bonds formed by the amino groups of Leu15, Tyr16 and Phe25 in the oxidized B-chain of insulin. In its main properties the enzyme is similar to metalloproteinases of animal and bacterial origin.

Revealing genetic diversity in land races and wild accessions of mungbean [Vigna radiata (L.) Wilczek] using SDS-PAGE of seed storage proteins

2015 ◽  
Author(s):  
Ananya Panda ◽  
Swapan K. Tripathy
Keyword(s):  
Storage Proteins ◽  
Seed Storage Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Land Races ◽  
Sds Page ◽  
Wild Accessions ◽  
Total Seed

Total seed storage protein profiles of 74 mungbean land races, three wild accessions and a popular variety ‘Jyoti’ of Odisha were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 32 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into eight protein types. Genotypes included in each protein type had 100% homology and some of these could be duplicates. In this pursuit, a few specific polypeptide markers have been detected for identification of the land races/ genotypes. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 70% phenon level. Paralakhemundi local, Samarjhola local and Phulbani local-D; and three wild accessions (TCR 20, TCR 213 and TCR 243) were comparatively divergent from other genotypes. Besides, Jyoti, Kalahandi local 2A, Sikri local, kodala local A and TCR 20 were identified to be protein rich with high seed yield. TCR 20 being morphologically similar to mungbean, moderately high protein content and high yielding as well as resistant to drought and bruchids; it may serve as a valuable source genotype in recombination breeding

scholarly journals Characterization of seed storage proteins in high protein genotypes of cowpea [Vigna unguiculata (L.) Walp.]

2010 ◽  
Vol 16 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Prachi Gupta ◽  
Rohtas Singh ◽  
S. Malhotra ◽  
K. S. Boora ◽  
H. R. Singal
Keyword(s):  
Vigna Unguiculata ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
High Protein
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