scholarly journals Characterization of Tunisian castor bean genotypes using SDS-PAGE of total seed storage proteins

10.5219/973 ◽  
2018 ◽  
Vol 12 (1) ◽  
Author(s):  
Martin Vivodík ◽  
Ezzeddine Saadaoui ◽  
Zdenka Gálová ◽  
Želmíra Balážová ◽  
Lenka Petrovičová
Keyword(s):  
Castor Bean ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Sds Page ◽  
Total Seed

Revealing genetic diversity in land races and wild accessions of mungbean [Vigna radiata (L.) Wilczek] using SDS-PAGE of seed storage proteins

2015 ◽  
Author(s):  
Ananya Panda ◽  
Swapan K. Tripathy
Keyword(s):  
Storage Proteins ◽  
Seed Storage Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Land Races ◽  
Sds Page ◽  
Wild Accessions ◽  
Total Seed

Total seed storage protein profiles of 74 mungbean land races, three wild accessions and a popular variety ‘Jyoti’ of Odisha were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 32 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into eight protein types. Genotypes included in each protein type had 100% homology and some of these could be duplicates. In this pursuit, a few specific polypeptide markers have been detected for identification of the land races/ genotypes. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 70% phenon level. Paralakhemundi local, Samarjhola local and Phulbani local-D; and three wild accessions (TCR 20, TCR 213 and TCR 243) were comparatively divergent from other genotypes. Besides, Jyoti, Kalahandi local 2A, Sikri local, kodala local A and TCR 20 were identified to be protein rich with high seed yield. TCR 20 being morphologically similar to mungbean, moderately high protein content and high yielding as well as resistant to drought and bruchids; it may serve as a valuable source genotype in recombination breeding

scholarly journals Isolation and properties of a metalloproteinase from buckwheat (Fagopyrum esculentum) seeds

1990 ◽  
Vol 272 (3) ◽  
pp. 677-682 ◽  
Author(s):  
M A Belozersky ◽  
Y E Dunaevsky ◽  
N E Voskoboynikova
Keyword(s):  
Storage Proteins ◽  
Limited Proteolysis ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Fagopyrum Esculentum ◽  
Gel Chromatography ◽  
Ph Optimum ◽  
Peptide Bonds ◽  
Sds Page ◽  
Homogeneous Preparation

A homogeneous preparation of metalloproteinase, purified 1000-fold, was obtained from buckwheat (Fagopyrum esculentum) seeds. The Mr of the enzyme, determined by SDS/PAGE, was 34,000 (it was 39,000 by gel chromatography). Its pH optimum was 8.0-8.2 with 13 S globulin, from buckwheat seeds, as substrate. Atomic-absorption spectroscopy revealed the presence of one Zn2+ ion per enzyme molecule. The enzyme was completely inhibited by EDTA (1 mM), zincone (1 mM) and 1, 10-phenanthroline (1 mM). The metalloproteinase performed limited proteolysis of the following seed storage proteins: 13 S globulin from buckwheat seeds and 11 S globulin from soybean (Glycine max) seeds. It hydrolysed three peptide bonds formed by the amino groups of Leu15, Tyr16 and Phe25 in the oxidized B-chain of insulin. In its main properties the enzyme is similar to metalloproteinases of animal and bacterial origin.

Characterization of spelt (Triticum spelta L.) forms by gel electrophoretic analyses of seed storage proteins. I. The gliadins

1997 ◽  
Vol 94 (1) ◽  
pp. 52-60 ◽  
Author(s):  
S. Harsch ◽  
T. Günther ◽  
B. Rozynek ◽  
C. U. Hesemann ◽  
Ch. I. Kling
Keyword(s):  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Spelta

scholarly journals Characterization of seed storage proteins in high protein genotypes of cowpea [Vigna unguiculata (L.) Walp.]

2010 ◽  
Vol 16 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Prachi Gupta ◽  
Rohtas Singh ◽  
S. Malhotra ◽  
K. S. Boora ◽  
H. R. Singal
Keyword(s):  
Vigna Unguiculata ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
High Protein

scholarly journals Phytochemical Evaluation of Moth Bean (Vigna aconitifoliaL.) Seeds and Their Divergence

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Neha Gupta ◽  
Nidhi Shrivastava ◽  
Pramod Kumar Singh ◽  
Sameer S. Bhagyawant
Keyword(s):  
Storage Proteins ◽  
Seed Storage Protein ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Molecular Weight Range ◽  
Two Dimensional Gel Electrophoresis ◽  
Moth Bean ◽  
Total Seed

In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE) was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3–10 in a molecular weight range of 11–170 kDa. In both seed accessions maximum protein spots are seen in the pI range of 6–8.

Comparison of Moringa oleifera oils extracted with supercritical fluids and hexane and characterization of seed storage proteins in defatted flour

2021 ◽  
Vol 40 ◽  
pp. 100830
Author(s):  
Jorge Martín Guzmán-Albores ◽  
Esaú Bojórquez-Velázquez ◽  
Antonio De León-Rodríguez ◽  
Oscar de Jesús Calva-Cruz ◽  
Ana Paulina Barba de la Rosa ◽  
...  
Keyword(s):  
Supercritical Fluids ◽  
Moringa Oleifera ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins

scholarly journals Study on Seed Protein and Protein Profile Pattern of Chickpea (Cice arietinum L.) by SDS-PAGE under Drought Stress and Fertilization

2015 ◽  
Vol 9 (5) ◽  
pp. 87-90 ◽  
Author(s):  
Vahid Ashrafi ◽  
Hoorieh Pourbozorg ◽  
Nasroallah Moradi Kor ◽  
Abasalt Rostami Ajirloo ◽  
Morteza Shamsizadeh ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Protein ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Protein Yield ◽  
Severe Drought ◽  
Stress Treatment ◽  
Sds Page ◽  
Effects Of Drought

A field experiment was performed in order to evaluate the effects of drought stress and application of starter nitrogen fertilizer on seed storage proteins and protein pattern in chickpea cultivars. Experiment was performed in split-factorial using randomized complete block design with three replications. The experiment was laid out in a split-factorial design with drought stress in main plots and cultivar with nitrogen fertilizer in subplots with three replications. The experimental treatments consisted of three levels of drought stress [severe drought stress (S2), moderate drought stress (S1) and no drought stress(S0)] and two cultivars of chickpea consist of Azad and Bivanij and 2 N levels. Plants were either not given any N fertilizer (N0), or fertilized by N fertilizer at the rate of 25 kg ha-1 (N25). The results of this study showed that the effects of drought stress on seed storage proteins and protein yield, effect of cultivars on protein yield were significant. With increase of drought stress seed storage proteins was increased and protein yield decreased. Severe drought stress treatment has the highest seed protein and the control treatment has the lowest seed protein. Non stress treatment has the highest protein yield and the severe  drought stress treatment has the lowest protein yield. The SDS- PAGE results revealed that no effects treatments on the protein banding patterns but the related severe drought stress bands were chromatic, because they have highest protein concentration in some protein bands.DOI: http://dx.doi.org/10.3126/ijls.v9i5.12704

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