Comparison of the Effects of Arsenic (V), Cadmium (II), and Mercury (II) Single Metal and Mixed Metal Exposure in Radish, Raphanus sativus, Fescue Grass, Festuca ovina, and Duckweed, Lemna minor

ABSTRACT Leachate from a municipal battery recycling site is a potent source of mixed-metal released into the environment. The present study investigated the degree at which mixed-metal exposure to the municipal auto-battery leachate (MABL) and to the Elewi Odo municipal auto-battery recycling site leachate (EOMABRL) affected the lipid membrane of the testes in in vitro experiment. The results showed elevated level of mixed-metals over the permissible levels in drinking water, as recommended by regulatory authorities. In the leachate samples, the levels of malondialdehyde (MDA), a biomarker of lipid damage, was significantly (p<0.05) increased in rat testes in a dose-dependent manner. MDA induced by the municipal auto-battery leachate (MABL) was significantly (p<0.05) higher than the leachate from Elewi Odo municipal auto-battery recycling site (EOMABRL). The testicular lipid membrane capacity was compromised following treatment with leachate from the municipal battery recycling site, implicating mixed-metal exposure as the causative agent of testicular damage and male infertility.

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Copper/Metal Ratios in the Gills of Rainbow Trout (Oncorhynchus mykiss) Provide Evidence of Copper Exposure Under Conditions of Mixed-Metal Exposure

Archives of Environmental Contamination and Toxicology ◽

10.1007/s00244-003-3030-9 ◽

2004 ◽

Vol 47 (1) ◽

Cited By ~ 2

Author(s):

R. W. Daglish ◽

B. F. Nowak ◽

T. W. Lewis

Keyword(s):

Rainbow Trout ◽

Oncorhynchus Mykiss ◽

Metal Exposure ◽

Copper Exposure ◽

Copper Metal ◽

Mixed Metal ◽

Rainbow Trout Oncorhynchus Mykiss ◽

Metal Ratios

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TOXICIDAD DE CINCO EXTRACTOS ACUOSOS BOTÁNICOS SOBRE PANAGRELLUS REDIVIVUS (NEMATODA: PANAGROLAIMIDAE), DAPHNIA MAGNA (CRUSTACEA: DAPHNIIDAE), LEMNA MINOR (ARACEAE) Y RAPHANUS SATIVUS (BRASSICACEAE)

Neotropical Helminthology ◽

10.24039/rnh2017111700 ◽

2020 ◽

Vol 11 (1) ◽

Author(s):

Hildebrando Ayala ◽

José Iannacone ◽

Lorena Alvariño

Keyword(s):

Daphnia Magna ◽

Eucalyptus Globulus ◽

Raphanus Sativus ◽

Lemna Minor ◽

Ricinus Communis ◽

Ruta Graveolens ◽

Panagrellus Redivivus ◽

Urtica Urens

El impacto toxicológico de extractos botánicos en el ambiente acuático y terrestre es un área de investigación emergente a nivel global. Cinco extractos botánicos de eucalipto (Eucalyptus globulus, Myrtaceae), ruda (Ruta graveolens, Rutaceae), ortiga (Urtica urens, Urticaceae), muña (Minthostachys mollis, Lamiaceae) e higuerilla (Ricinus communis, Euphorbiaceae) con potencial para el control de plagas fueron evaluados sobre el microgusano de la avena Panagrellus redivivus, la pulga del agua Daphnia magna, la lenteja de agua Lemna minor y el rábano Raphanus sativus en bioensayos toxicológicos bajo condiciones de laboratorio. Daphnia magna en términos de CL a 48 h de exposición 50 fue más sensible a E. globulus y a R. communis. P. redivivus a 96 h de exposición fue más sensible a R. communis. La clorosis de L. minor a 96 h de exposición y la inhibición de la germinación en términos de CE a 96 h de exposición sobre R. sativus fueron más sensibles a R. graveolens. Minthostachys mollis 50 ocasionó la menor toxicidad en los cuatro modelos biológicos. Los extractos botánicos de eucalipto e higuerilla causan una mayor toxicidad en el ambiente acuático y en el ambiente terrestre el extracto acuoso de ruda produjo mayor efecto tóxico.

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Metal-specific induction of metallothionein isoforms in the blue crab Callinectes sapidus in response to single- and mixed-metal exposure

Archives of Biochemistry and Biophysics ◽

10.1016/0003-9861(92)90712-6 ◽

1992 ◽

Vol 294 (2) ◽

pp. 461-468 ◽

Cited By ~ 42

Author(s):

Marius Brouwer ◽

Daniel Schlenk ◽

Amy Huffman Ringwood ◽

Thea Brouwer-Hoexum

Keyword(s):

Blue Crab ◽

Callinectes Sapidus ◽

Metal Exposure ◽

Mixed Metal ◽

Metallothionein Isoforms ◽

Specific Induction

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Low temperature x-ray microanalysis of frozen-hydrated tobacco leaves

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111537 ◽

1984 ◽

Vol 42 ◽

pp. 284-285

Author(s):

S. Edith Taylor ◽

Patrick Echlin ◽

May McKoon ◽

Thomas L. Hayes

Keyword(s):

Low Temperature ◽

Lemna Minor ◽

Root Tips ◽

Tobacco Leaves ◽

X Ray ◽

Whole Cells ◽

Carbon Coated ◽

The Right ◽

Beam Currents ◽

The University

Low temperature x-ray microanalysis (LTXM) of solid biological materials has been documented for Lemna minor L. root tips. This discussion will be limited to a demonstration of LTXM for measuring relative elemental distributions of P,S,Cl and K species within whole cells of tobacco leaves.Mature Wisconsin-38 tobacco was grown in the greenhouse at the University of California, Berkeley and picked daily from the mid-stalk position (leaf #9). The tissue was excised from the right of the mid rib and rapidly frozen in liquid nitrogen slush. It was then placed into an Amray biochamber and maintained at 103K. Fracture faces of the tissue were prepared and carbon-coated in the biochamber. The prepared sample was transferred from the biochamber to the Amray 1000A SEM equipped with a cold stage to maintain low temperatures at 103K. Analyses were performed using a tungsten source with accelerating voltages of 17.5 to 20 KV and beam currents from 1-2nA.

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Elemental Analysis of Phloem Tissue in Lemna Minor Root Tips

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600003470 ◽

1980 ◽

Vol 38 ◽

pp. 798-799

Author(s):

Patrick Echlin ◽

Thomas Hayes ◽

Clifford Lai ◽

Greg Hook

Keyword(s):

Vascular Tissue ◽

Lemna Minor ◽

Atomic Weight ◽

Companion Cell ◽

Root Tips ◽

Phloem Tissue ◽

Cell Stage ◽

Phloem Parenchyma ◽

Parenchyma Cells ◽

Xylem Element

Studies (1—4) have shown that it is possible to distinguish different stages of phloem tissue differentiation in the developing roots of Lemna minor by examination in the transmission, scanning, and optical microscopes. A disorganized meristem, immediately behind the root-cap, gives rise to the vascular tissue, which consists of single central xylem element surrounded by a ring of phloem parenchyma cells. This ring of cells is first seen at the 4-5 cell stage, but increases to as many as 11 cells by repeated radial anticlinal divisions. At some point, usually at or shortly after the 8 cell stage, two phloem parenchyma cells located opposite each other on the ring of cells, undergo an unsynchronized, periclinal division to give rise to the sieve element and companion cell. Because of the limited number of cells involved, this developmental sequence offers a relatively simple system in which some of the factors underlying cell division and differentiation may be investigated, including the distribution of diffusible low atomic weight elements within individual cells of the phloem tissue.

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