Isolation of the constituents and evaluation of allelopathic potential of Raphanus sativus L. (Brassicaceae)

EFECTO ALEPOPÁTICO DE TRES EXTRACTOS VEGETALES SOBRE EL DESARROLLO DE RAPHANUS SATIVUS (BRASSICACEAE) EN EL PERÚ ALLELOPATHIC EFFECT OF THREE PLANTS EXTRACTS ON THE DEVELOPMENT OF RAPHANUS SATIVUS (BRASSICACEAE) IN PERÚ Hildebrando Ayala & Rafael La Rosa DOI: https://doi.org/10.33017/RevECIPeru2007.0007/ RESUMEN El uso de plantas con actividad alelopática en la actualidad resulta de gran importancia porque nos permite el desarrollo de una agricultura rentable y no contaminante del medio ambiente. El objetivo de este trabajo fue estudiar la acción de extractos acuosos de ajo (Allium sativum L, Liliaceae), palta (Persea americana Mill, Lauraceae) y ruda (Ruta graveolens L, Rutaceae) sobre el desarrollo de la hortaliza rabanito (Raphanus sativus L, Brassicaceae). Los extractos se prepararon a partir de bulbos de A. sativum, semilla de P. americana y hojas y flores de R. graveolens a los cuales triturados se le adiciona agua hervida. Los tratamientos fueron extractos puros al (20% p/v). El diseño fue completamente aleatorizado con 4 repeticiones. Se determino peso fresco (g), peso seco (g), longitud de raíz (cm), longitud área (cm) y área foliar(cm2). Los datos se analizaron mediante ANOVA y Test de Tukey para la comparación de medias. Los resultados alcanzados muestran de que no existe efectos significativos en comparación con el control, del potencial alelopático de los extractos acuosos evaluados bajo condiciones controladas. Palabras claves: Extractos acuosos, semillas, hojas, flores, bulbos, Allium sativum, Persea americana, Ruta graveolens. ABSTRACT The use of plants with allelopathic activity nowadays is very important because it allows us to develop a profitable agriculture and non-polluting the environment. The objective of this work was to study the action of aqueous extracts of garlic (Allium sativum L, Liliaceae), avocado (Persea americana Mill, Lauraceae) and rough (Ruta graveolens L, Rutaceae) on the development of vegetable radish (Raphanus sativus L, Brassicaceae). The extracts were prepared from A. sativum bulbs, P. americana seeds and R. graveolens leaves and flowers to which he adds crushed boiled water. Treatments were the pure extracts (20% w / v). The design was completely randomized with 4 repetitions. It was found fresh weight (g), dry weight (g), root length (cm) long area (cm) and leaf area (cm2 ). The data was analyzed with Anova and Tukey test for the comparison of averages. The results show that there is no significant effects compared to the control of allelopathic potential of aqueous extracts tested under controlled conditions. Keywords: Aqueous extracts, seed, leaves, flowers, bulbs, Allium sativum, Persea americana, Ruta graveolens.

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Methods of preparing aqueous extract and bioactivity of Mentha spicata L. on Raphanus sativus L. seeds

Acta Iguazu ◽

10.48075/actaiguaz.v9i4.25680 ◽

2020 ◽

Vol 9 (4) ◽

pp. 118-130

Author(s):

Leonardo Pereira Xavier ◽

Andrea Pires ◽

Lisandro Tomas da Silva Bonome ◽

Gabriela Silva Moura ◽

Gilmar Franzener ◽

...

Keyword(s):

Phenolic Compounds ◽

Aqueous Extract ◽

Cover Crops ◽

Weed Management ◽

Raphanus Sativus ◽

Extraction Methods ◽

Mentha Spicata ◽

Allelopathic Potential ◽

Raphanus Sativus L ◽

Physiological Quality

The plant interaction knowledge can be applied in several sectors in agriculture such as crop rotation, weed management, green manure and the use of cover crops. Studies on the allelopathic potential of certain plants have been carried out with the application of vegetable extracts on the seeds of a sensitive plant, however, a consensus has not been reached regarding the best way to prepare such extracts. This study aimed to evaluate the efficacy of methods of extraction of allelopathic compounds and the bioactivity of different concentrations of the Mentha spicata L. aqueous extract on Raphanus sativus L. seeds. A completely randomized design was used in a 5x4 factorial scheme with four replications and four extraction methods (infusion, grinding in a blender, drying at 40 °C and 70 °C) and five concentrations (0; 25; 50; 75 and 100%). The variables investigated were: phenolic compounds quantification, germination, germination speed index (GSI), mean germination time (MGT), radish shoot and root length. The extract presenting the highest amount of phenolic compounds was prepared via infusion (724.36 µg/mL), followed by drying at 40 °C (605.07 µg/mL), blending (594.12 µg/mL) and drying at 70 °C (529.36 µg/mL). The Mentha spicata L. aqueous extracts interfered in the radish seed physiological quality, by inhibiting germination, GSI and MGT increase, mainly when in higher concentrations. There was a directly proportional relation between the quantity of phenolic compounds extracted through different extraction methods and the interference in the physiological quality of the radish seeds.

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Allelopathic Potential of the Aqueous Extract of Raphanus sativus L. on the Germination of Beans and Corn Seeds

OALib ◽

10.4236/oalib.1103590 ◽

2017 ◽

Vol 04 (05) ◽

pp. 1-10

Author(s):

Marli de Moraes Gomes ◽

Douglas Junior Bertoncelli ◽

Guilherme Augusto Cito Alves ◽

Gustavo Henrique Freiria ◽

Felipe Favoretto Furlan ◽

...

Keyword(s):

Aqueous Extract ◽

Raphanus Sativus ◽

Allelopathic Potential ◽

Raphanus Sativus L

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Phenolic Compounds with Allelopathic Potential of Secale cereale L. and Raphanus sativus L. Grown Under an Agroecological No-Tillage System

Planta Daninha ◽

10.1590/s0100-83582019370100090 ◽

2019 ◽

Vol 37 ◽

Author(s):

M. SOUZA ◽

J.J. COMIN ◽

C. KURTZ ◽

P.E. LOVATO ◽

A.P. LIMA ◽

...

Keyword(s):

Phenolic Compounds ◽

Cover Crops ◽

Secale Cereale ◽

Raphanus Sativus ◽

No Tillage ◽

Allelopathic Potential ◽

Oilseed Radish ◽

Raphanus Sativus L ◽

Secale Cereale L ◽

Weed Emergence

ABSTRACT: The identification of compounds with allelopathic potential produced by cover crops can assist in selecting species for weed management purposes in no-tillage systems. This study aimed to identify the main phenolic compounds with allelopathic potential in the shoot of rye (Secale cereale L.) and oilseed radish (Raphanus sativus L.) cover crops, as well as evaluate whether the cultivation system and phenological stage may influence secondary metabolite production and weed emergence. Samples of the shoot of these cover crops were collected at 60, 80, and 100 days after sowing (DAS) and 15 and 30 days after lodging (DAL) under field conditions. Weed emergence was evaluated at 45, 75, and 100 DAL of cover crops. The main compounds in rye were 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA) under monocropping and intercropping, while flavonoid quercetin was found in oilseed radish at all evaluated times. During the growing cycle, the highest contents of phenolic compounds were found at the elongation stage (60 DAS) of rye under monocropping and intercropping systems (9.33 and 8.22 mg g-1 DM, respectively) and at grain filling stage (100 DAS) for oilseed radish intercropped with rye and black oat (3.24 and 3.83 mg g-1 DM, respectively). No differences were found in the contents of the main compounds when the species was grown under monocropping or intercropping systems. A reduction in the contents of MBOA, BOA, and quercetin was observed after lodging. Weed dry matter production was lower at 45 DAL in all treatments with rye and oilseed radish residues when compared to the control. The intercropping of rye with oilseed radish is an alternative management for weed control in agroecological systems due to the physical barrier created by these species and the presence of phenolic compounds with allelopathic potential.

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