Suppression of acrylamide toxicity by carboxyfullerene in human neuroblastoma cells in vitro

2009 ◽  
Vol 83 (9) ◽  
pp. 817-824 ◽  
Author(s):  
Tomoyuki Sumizawa ◽  
Hideki Igisu
Keyword(s):  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma

scholarly journals In vitro targeting and specific transfection of human neuroblastoma cells by chCE7 antibody-mediated gene transfer

Gene Therapy ◽  
1997 ◽  
Vol 4 (2) ◽  
pp. 156-161 ◽  
Author(s):  
J-L Coll ◽  
E Wagner ◽  
V Combaret ◽  
K Metchler ◽  
H Amstutz ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma

scholarly journals Human neuroblastoma cells acquire regulated secretory properties and different sensitivity to Ca2+ and alpha-latrotoxin after exposure to differentiating agents.

1989 ◽  
Vol 108 (6) ◽  
pp. 2291-2300 ◽  
Author(s):  
E Sher ◽  
S Denis-Donini ◽  
A Zanini ◽  
C Bisiani ◽  
F Clementi
Keyword(s):  
Electron Microscopy ◽  
De Novo ◽  
Neuroblastoma Cells ◽  
Calcium Ionophore ◽  
Membrane Receptors ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Adequate Model ◽  
Human Neurons

IMR-32 human neuroblastoma cells are unable to release [3H]dopamine in response to secretagogues. However, they express a normal complement of membrane receptors and ion channels which are efficiently coupled to second messenger production. In the present study we took advantage of the ability of this cell line to differentiate in vitro in the presence of either dibutyrryl-cAMP or 5-bromodeoxyuridine, to analyze any developmentally regulated changes in its secretory properties. Uptake, storage, and release of [3H]dopamine were studied biochemically and by autoradiography. The calcium ionophore ionomycin, phorbol 12-myristate 13-acetate and the presynaptic acting neurotoxin alpha-latrotoxin were used in both control and differentiated cells as secretagogue agents. The presence of secretory organelles was investigated by electron microscopy; the expression of secretory organelle markers, such as chromogranin/secretogranin proteins (secretory proteins) and synaptophysin (membrane protein), was detected by Western blotting and immunofluorescence. The results obtained indicate that IMR-32 cells acquire regulated secretory properties after in vitro drug-induced differentiation: (a) they assemble "de novo" secretory organelles, as revealed by electron microscopy and detection of secretory organelle markers, and (b) they are able to store [3H]dopamine and to release the neurotransmitter in response to secretagogue stimuli. Furthermore, secretagogue sensitivity was found to be different, depending on the differentiating agent. In fact, dibutyrryl-cAMP treated cells release [3H]dopamine in response to alpha-latrotoxin, but not in response to ionomycin, whereas 5-bromodeoxyuridine treated cells release the neurotransmitter in response to both secretagogues. All together these results suggest that IMR-32 cells represent an adequate model for studying the development of the secretory apparatus in cultured human neurons.

scholarly journals The Impact of African and Brazilian Zika virus isolates on neuroprogenitors

2016 ◽  
Author(s):  
Loraine Campanati ◽  
Luiza M. Higa ◽  
Rodrigo Delvecchio ◽  
Paula Pezzuto ◽  
Ana Luiza Valadão ◽  
...  
Keyword(s):  
Zika Virus ◽  
Neuroblastoma Cells ◽  
Brazilian Isolate ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Differentiation Potential ◽  
Neuronal Progenitors ◽  
The Impact ◽  
Virus Isolates

AbstractIn the last few months, an overwhelming number of people have been exposed to the Zika virus (ZIKV) in South and Central America. Here we showed, in vitro, that a Brazilian isolate impacts more severely murine neuronal progenitors and neurons than the African strain MR766. We found that the Brazilian isolate more pronouncedly inhibits neurite extension from neurospheres, alters their differentiation potential and causes neurons to have less and shorter processes. Comparing both lineages using a panel of inflammatory cytokines, we showed, with human neuroblastoma cells, that ZIKV induces the production of several inflammatory and chemotactic cytokines and once again, the Brazilian isolate had a more significant impact. Although much more needs to be studied regarding the association of ZIKV infection and brain damage during development, our study sheds some light into the differences between African and American lineages and the mechanisms by which the virus may be affecting neurogenesis.

scholarly journals Squalamine and Its Derivatives Modulate the Aggregation of Amyloid-β and α-Synuclein and Suppress the Toxicity of Their Oligomers

2021 ◽  
Vol 15 ◽  
Author(s):  
Ryan Limbocker ◽  
Roxine Staats ◽  
Sean Chia ◽  
Francesco S. Ruggeri ◽  
Benedetta Mannini ◽  
...  
Keyword(s):  
Small Molecules ◽  
Neuroblastoma Cells ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Parkinson’S Diseases ◽  
Wide Range ◽  
Opposing Effects

The aberrant aggregation of proteins is a key molecular event in the development and progression of a wide range of neurodegenerative disorders. We have shown previously that squalamine and trodusquemine, two natural products in the aminosterol class, can modulate the aggregation of the amyloid-β peptide (Aβ) and of α-synuclein (αS), which are associated with Alzheimer’s and Parkinson’s diseases. In this work, we expand our previous analyses to two squalamine derivatives, des-squalamine and α-squalamine, obtaining further insights into the mechanism by which aminosterols modulate Aβ and αS aggregation. We then characterize the ability of these small molecules to alter the physicochemical properties of stabilized oligomeric species in vitro and to suppress the toxicity of these aggregates to varying degrees toward human neuroblastoma cells. We found that, despite the fact that these aminosterols exert opposing effects on Aβ and αS aggregation under the conditions that we tested, the modifications that they induced to the toxicity of oligomers were similar. Our results indicate that the suppression of toxicity is mediated by the displacement of toxic oligomeric species from cellular membranes by the aminosterols. This study, thus, provides evidence that aminosterols could be rationally optimized in drug discovery programs to target oligomer toxicity in Alzheimer’s and Parkinson’s diseases.

Sign in / Sign up

Export Citation Format

Share Document