Selective extraction of phospholipids from dairy products by micro-solid phase extraction based on titanium dioxide microcolumns followed by MALDI-TOF-MS analysis

2009 ◽  
Vol 394 (5) ◽  
pp. 1453-1461 ◽  
Author(s):  
Cosima D. Calvano ◽  
Ole N. Jensen ◽  
Carlo G. Zambonin
Keyword(s):  
Titanium Dioxide ◽  
Solid Phase Extraction ◽  
Dairy Products ◽  
Solid Phase ◽  
Selective Extraction ◽  
Phase Extraction ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Monitoring Activity-Dependent Peptide Release from the CNS Using Single-Bead Solid-Phase Extraction and MALDI TOF MS Detection

2005 ◽  
Vol 77 (6) ◽  
pp. 1580-1587 ◽  
Author(s):  
Nathan G. Hatcher ◽  
Timothy A. Richmond ◽  
Stanislav S. Rubakhin ◽  
Jonathan V. Sweedler
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Peptide Release ◽  
Single Bead ◽  
Activity Dependent ◽  
Monitoring Activity ◽  
Tof Ms

scholarly journals Hexagonal Mesoporous Silica as a Rapid, Efficient and Versatile Tool for MALDI-TOF MS Sample Preparation in Clinical Peptidomics Analysis: A Pilot Study

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2311
Author(s):  
Rosa Terracciano ◽  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Corrado Pelaia ◽  
Rocco Savino
Keyword(s):  
Synovial Fluid ◽  
Mesoporous Silica ◽  
Sample Preparation ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Versatile Tool ◽  
Tof Ms

Improvement in high-throughput MALDI-TOF MS analysis requires practical and efficient sample preparation protocols for high acquisition rates. The use of hexagonal mesoporous silica (HMS) sorbents in combination with MALDI-TOF MS was explored as a versatile tool for peptidomic profiling of clinical specimens difficult to process, but considered important sources of disease biomarkers: synovial fluid and sputum. A rapid and efficient procedure, based on dispersive solid-phase extraction of peptides using commercially available wormhole mesostructured HMS, was tested for: a) pre-concentration of standard peptides in serially diluted solution up to the sub-nanomolar range; b) peptidome profiling of sputum and synovial fluid. The use of HMS, as dispersed sponges, significantly amplified the peptidic repertoire of sputum and synovial fluid by excluding from the adsorptive process large size proteins, which mask and/or suppress peptidome signals. The protocol proposed, as dispersive solid phase extraction, ensures good analytical performances. Moreover, it is economical and rapid, as it avoids the use of less reproducible and prolonged sample preparation procedures, such as the use of ultrafiltration filter devices. These findings may contribute to defining a high-throughput screening MS-based platform for monitoring key peptidic features of difficult to analyse bodily fluids in a clinical setting.

scholarly journals Sensitive determination of gentamicin in plasma using ion-exchange solid-phase extraction followed by UHPLC-MS/MS analysis

2021 ◽  
pp. e00246
Author(s):  
Ana Laura Anibaletto dos Santos ◽  
Anne Caroline Cezimbra da Silva ◽  
Lilian de Lima Feltraco Lizot ◽  
Anelise Schneider ◽  
Roberta Zilles Hahn ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Ms Analysis ◽  
Sensitive Determination

The suitability of different DHB isomers as matrices for the MALDI-TOF MS analysis of phospholipids: which isomer for what purpose?

2006 ◽  
Vol 36 (4-5) ◽  
pp. 517-527 ◽  
Author(s):  
Jürgen Schiller ◽  
Rosmarie Süß ◽  
Beate Fuchs ◽  
Matthias Müller ◽  
Marijana Petković ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Gas Chromatographic/Mass Spectrometric Confirmation of Leucomalachite Green in Catfish (Ictalurus punctatus) Tissue

1995 ◽  
Vol 78 (4) ◽  
pp. 971-977 ◽  
Author(s):  
Sherri B Turnipseed ◽  
José E Roybal ◽  
Jeffrey A Hurlbut ◽  
Austin R Long
Keyword(s):  
Solid Phase Extraction ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Mass Spectrometric ◽  
Phase Extraction ◽  
Neutral Alumina ◽  
Ms Analysis ◽  
Solid Phase Extraction Cartridge ◽  
Leucomalachite Green ◽  
Chromatographic Mass

Abstract A gas chromatographic/mass spectrometric (GC/MS) method was developed to confirm the presence of leucomalachite green (LMG), a metabolite of the triphenylmethane dye malachite green (MG), in catfish tissue. Residues were isolated according to a previously described liquid chromatographic (LC)A/IS spectrometric analysis of MG and LMG in fish. In our isolation procedure, analytes are extracted from tissue with acetonitrile–buffer, partitioned into CH2CI2, and applied to neutral alumina and propylsulfonic acid solid-phase extraction cartridges. Before GC/MS analysis, extracts prepared for the LC determinative method are eluted from a cyano solid-phase extraction cartridge, extracted into organic solvent, and concentrated for GC/MS analysis. Selected ion monitoring was performed by using 5 diagnostic ions (m/z 330,329,253,210, and 165) of LMG. The method was validated by confirming LMG in tissue fortified with mixtures of MG and LMG (5 and 10 ng/g each) and in tissue from fish that had been exposed to low levels of MG.

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