Antibodies Against Central Nervous System Tissue (Anti-Cns) Detected by Elisa and Western Blotting: Marker Antibodies for Neuropsychiatric Manifestations in Connective Tissue Diseases

Autoimmunity ◽  
1991 ◽  
Vol 10 (2) ◽  
pp. 133-144 ◽  
Author(s):  
Reinhild Klein ◽  
Christiane Richter ◽  
Peter A. Berg
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Connective Tissue ◽  
Western Blotting ◽  
Connective Tissue Diseases ◽  
Central Nervous System Tissue ◽  
Neuropsychiatric Manifestations

Comparison of Analytical Methods for the Detection of Central Nervous System Tissue in Ground Beef

2008 ◽  
Vol 71 (7) ◽  
pp. 1505-1509
Author(s):  
DONG GYUN LIM ◽  
CHEORUN JO ◽  
MOOHA LEE
Keyword(s):  
Spinal Cord ◽  
Central Nervous System ◽  
Nervous System ◽  
Western Blotting ◽  
Ground Beef ◽  
Enzyme Linked Immunosorbent Assay ◽  
Spongiform Encephalopathy ◽  
Spinal Cord Tissue ◽  
Central Nervous System Tissue ◽  
By Products

Bovine spongiform encephalopathy is most likely transmitted by the consumption of central nervous system tissue of infected cows. The objective of this study was to compare the sensitivity or limits of detection of two central nervous system tissue detection assays (glial fibrillary acidic protein enzyme-linked immunosorbent assay [ELISA] and neuron-specific enolase Western blotting assay) in by-products and ground beef. By-products including brain, spinal cord, and ileum were collected from the slaughterhouse and used for analyses with ELISA and Western blotting assays. Beef samples were prepared by mixing ground beef with different amounts of spinal cord tissue (0, 0.03, 0.06, and 0.1%) and were analyzed using the two central nervous system tissue detection methods. Both analytical assays were applicable in detecting central nervous system tissue in ground beef. However, the ELISA method was considered superior because of its ease of use, high sensitivity, and rapidity as compared with the Western blot method.

scholarly journals AB0081 Central nervous system involvement in connective tissue diseases – our expirience

2001 ◽  
Author(s):  
DZ Stefanovic ◽  
BC Glisic ◽  
MM Cirkovic ◽  
ZV Andjlekovic ◽  
MR Petronijevic ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Connective Tissue ◽  
Connective Tissue Diseases ◽  
Central Nervous System Involvement ◽  
System Involvement

Confocal laser microscopy of impregnated central nervous system tissue

1988 ◽  
Vol 46 ◽  
pp. 94-95
Author(s):  
J.N. Turner ◽  
M. Siemens ◽  
D. Szarowski ◽  
D.N. Collins
Keyword(s):  
Electron Microscopy ◽  
Central Nervous System ◽  
Nervous System ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Confocal Laser ◽  
High Contrast ◽  
Central Nervous System Tissue ◽  
Tissue Samples ◽  
Reflected Light

A classic preparation of central nervous system tissue (CNS) is the Golgi procedure popularized by Cajal. The method is partially specific as only a few cells are impregnated with silver chromate usualy after osmium post fixation. Samples are observable by light (LM) or electron microscopy (EM). However, the impregnation is often so dense that structures are masked in EM, and the osmium background may be undesirable in LM. Gold toning is used for a subtle but high contrast EM preparation, and osmium can be omitted for LM. We are investigating these preparations as part of a study to develop correlative LM and EM (particularly HVEM) methodologies in neurobiology. Confocal light microscopy is particularly useful as the impregnated cells have extensive three-dimensional structure in tissue samples from one to several hundred micrometers thick. Boyde has observed similar preparations in the tandem scanning reflected light microscope (TSRLM).

scholarly journals Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry

2012 ◽  
Vol 403 (7) ◽  
pp. 1851-1861 ◽  
Author(s):  
Yuki Sugiura ◽  
Nobuhiro Zaima ◽  
Mitsutoshi Setou ◽  
Seiji Ito ◽  
Ikuko Yao
Keyword(s):  
Mass Spectrometry ◽  
Central Nervous System ◽  
Nervous System ◽  
Imaging Mass Spectrometry ◽  
Central Nervous System Tissue ◽  
Tissue Sections

HISTOPATHOLOGY OF MULTIPLE SCLEROSIS LESIONS DETECTED BY MAGNETIC RESONANCE IMAGING IN UNFIXED POSTMORTEM CENTRAL NERVOUS SYSTEM TISSUE

Brain ◽  
1991 ◽  
Vol 114 (2) ◽  
pp. 1013-1023 ◽  
Author(s):  
J. NEWCOMBE ◽  
C. P. HAWKINS ◽  
C. L. HENDERSON ◽  
H. A. PATEL ◽  
M. N. WOODROOFE ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Nervous System ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Central Nervous System Tissue

Rapid test for the preclinical postmortem diagnosis of BSE in central nervous system tissue

2001 ◽  
Vol 149 (19) ◽  
pp. 577-582 ◽  
Author(s):  
J. Grassi ◽  
S. Simon ◽  
C. Crminon ◽  
Y. Frobert ◽  
E. Comoy ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Rapid Test ◽  
Central Nervous System Tissue ◽  
Postmortem Diagnosis
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