Community Structure, Abundance, and Activity of Methanotrophs in the Zoige Wetland of the Tibetan Plateau

Abstract Aims Evapotranspiration (ET) is an important component of the terrestrial water cycle and is easily affected by external disturbances, such as climate change and grazing. Identifying ET responses to grazing is instructive for determining grazing activity and informative for understanding the water cycle. Methods This study utilized 2 years (2014 and 2017) of eddy covariance data to test how grazing regulated ET for an alpine meadow ecosystem on the Tibetan Plateau (TP) by path analysis. Important Findings Radiation dominated ET with a decision coefficient of 64–74%. The soil water content (SWC) worked as the limiting factor in the fenced site. However, in the grazing site, the limiting factor was the vapor pressure deficit (VPD). Grazing had large effects on ET because it greatly affected the water conditions. The SWC and VPD were enhanced by 14.63% and 4.36% in the grazing site, respectively. Therefore, sufficient water was supplied to ET, especially during drought, and strengthened the transpiration pull. As a result, a favorable micrometeorological environment was created for ET. Grazing shifted the limiting factor of ET from the SWC to VPD, which weakened the limiting effect of the water conditions on ET and advanced the ET peak time. In addition, grazing altered the compositions of ET by changing the community structure, which directly resulted in an increased ET. In summary, grazing enhanced ET through altering the community structure and micrometeorological environments. The findings of this study further improve our understanding of the driving mechanisms of grazing on ET and will improve our predictions for the global water cycle.

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Control of Temperature on Microbial Community Structure in Hot Springs of the Tibetan Plateau

PLoS ONE ◽

10.1371/journal.pone.0062901 ◽

2013 ◽

Vol 8 (5) ◽

pp. e62901 ◽

Cited By ~ 76

Author(s):

Shang Wang ◽

Weiguo Hou ◽

Hailiang Dong ◽

Hongchen Jiang ◽

Liuqin Huang ◽

...

Keyword(s):

Community Structure ◽

Microbial Community ◽

Tibetan Plateau ◽

Microbial Community Structure ◽

Hot Springs ◽

The Tibetan Plateau

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Methanogenesis from Methanol at Low Temperatures by a Novel Psychrophilic Methanogen, “Methanolobus psychrophilus” sp. nov., Prevalent in Zoige Wetland of the Tibetan Plateau

Applied and Environmental Microbiology ◽

10.1128/aem.01146-08 ◽

2008 ◽

Vol 74 (19) ◽

pp. 6114-6120 ◽

Cited By ~ 78

Author(s):

Guishan Zhang ◽

Na Jiang ◽

Xiaoli Liu ◽

Xiuzhu Dong

Keyword(s):

Tibetan Plateau ◽

Methane Emission ◽

Low Temperatures ◽

Methanol Conversion ◽

Wetland Soil ◽

The Tibetan Plateau ◽

Hydrogenotrophic Methanogenesis ◽

Efficient Type ◽

Zoige Wetland ◽

A New Species

ABSTRACT The Zoige wetland of the Tibetan plateau is at permanent low temperatures and is a methane emission heartland of the plateau; however, cold-adaptive methanogens in the soil are poorly understood. In this study, a variety of methanogenic enrichments at 15ï¿½C and 30ï¿½C were obtained from the wetland soil. It was demonstrated that hydrogenotrophic methanogenesis was the most efficient type at 30ï¿½C, while methanol supported the highest methanogenesis rate at 15ï¿½C. Moreover, methanol was the only substrate to produce methane more efficiently at 15ï¿½C than at 30ï¿½C. A novel psychrophilic methanogen, strain R15, was isolated from the methanol enrichment at 15ï¿½C. Phylogenetic analysis placed strain R15 within the genus Methanolobus, loosely clustered with Methanolobus taylorii (96.7% 16S rRNA similarity). R15 produced methane from methanol, trimethylamine, and methyl sulfide and differed from other Methanolobus species by growing and producing methane optimally at 18ï¿½C (specific growth rate of 0.063 ï¿½ 0.001 h−1) and even at 0ï¿½C. Based on these characteristics, R15 was proposed to be a new species and named “Methanolobus psychrophilus” sp. nov. The Km and V max of R15 for methanol conversion were determined to be 87.5 ï¿½ 0.4 μM and 0.39 ï¿½ 0.04 mM h−1 at 18ï¿½C, respectively, indicating a high affinity and conversion efficiency for methanol. The proportion of R15 in the soil was determined by quantitative PCR, and it accounted for 17.2% ï¿½ 2.1% of the total archaea, enumerated as 107 per gram of soil; the proportion was increased to 42.4% ï¿½ 2.3% in the methanol enrichment at 15ï¿½C. This study suggests that the psychrophilic methanogens in the Zoige wetland are likely to be methylotrophic and to play a role in methane emission of the wetland.

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Response of Archaeal Community Structure to Environmental Changes in Lakes on the Tibetan Plateau, Northwestern China

Geomicrobiology Journal ◽

10.1080/01490450902892662 ◽

2009 ◽

Vol 26 (4) ◽

pp. 289-297 ◽

Cited By ~ 32

Author(s):

Hongchen Jiang ◽

Hailiang Dong ◽

Shicai Deng ◽

Bingsong Yu ◽

Qiuyuan Huang ◽

...

Keyword(s):

Community Structure ◽

Tibetan Plateau ◽

Environmental Changes ◽

Archaeal Community ◽

Northwestern China ◽

The Tibetan Plateau

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Warmer temperature accelerates methane emissions from the Zoige wetland on the Tibetan Plateau without changing methanogenic community composition

Scientific Reports ◽

10.1038/srep11616 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 35

Author(s):

Mengmeng Cui ◽

Anzhou Ma ◽

Hongyan Qi ◽

Xuliang Zhuang ◽

Guoqiang Zhuang ◽

...

Keyword(s):

Tibetan Plateau ◽

Community Composition ◽

Methane Emissions ◽

The Tibetan Plateau ◽

Methanogenic Community ◽

Zoige Wetland

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Analyzing the nitrate reductase gene (nirK) community in the peat soil of the Zoige Wetland of the Tibetan Plateau

Acta Ecologica Sinica ◽

10.5846/stxb201607151442 ◽

2017 ◽

Vol 37 (19) ◽

Author(s):

王蓥燕 WANG Yingyan ◽

卢圣鄂 LU Sheng'e ◽

陈小敏 CHEN Xiaomin ◽

李跃飞 LI Yuefei ◽

辜运富 GU Yunfu

Keyword(s):

Tibetan Plateau ◽

Nitrate Reductase ◽

Peat Soil ◽

The Tibetan Plateau ◽

Nitrate Reductase Gene ◽

Reductase Gene ◽

Zoige Wetland

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Bacterial community structure in soils of the Tibetan Plateau affected by discontinuous permafrost or seasonal freezing

Biology and Fertility of Soils ◽

10.1007/s00374-013-0869-4 ◽

2013 ◽

Vol 50 (3) ◽

pp. 555-559 ◽

Cited By ~ 12

Author(s):

Julien Ollivier ◽

Sizhong Yang ◽

Corina Dörfer ◽

Gerhard Welzl ◽

Peter Kühn ◽

...

Keyword(s):

Community Structure ◽

Tibetan Plateau ◽

Bacterial Community ◽

Bacterial Community Structure ◽

The Tibetan Plateau ◽

Seasonal Freezing ◽

Discontinuous Permafrost

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Prokaryotic Community Structure Driven by Salinity and Ionic Concentrations in Plateau Lakes of the Tibetan Plateau

Applied and Environmental Microbiology ◽

10.1128/aem.03332-15 ◽

2016 ◽

Vol 82 (6) ◽

pp. 1846-1858 ◽

Cited By ~ 36

Author(s):

Zhi-Ping Zhong ◽

Ying Liu ◽

Li-Li Miao ◽

Fang Wang ◽

Li-Min Chu ◽

...

Keyword(s):

Community Structure ◽

Tibetan Plateau ◽

Community Composition ◽

Qaidam Basin ◽

Nitrogen Concentration ◽

Distribution Patterns ◽

The Tibetan Plateau ◽

Prokaryotic Community ◽

Content Type ◽

Ionic Concentrations

ABSTRACTThe prokaryotic community composition and diversity and the distribution patterns at various taxonomic levels across gradients of salinity and physiochemical properties in the surface waters of seven plateau lakes in the Qaidam Basin, Tibetan Plateau, were evaluated using Illumina MiSeq sequencing. These lakes included Lakes Keluke (salinity, <1 g/liter), Qing (salinity, 5.5 to 6.6 g/liter), Tuosu (salinity, 24 to 35 g/liter), Dasugan (salinity, 30 to 33 g/liter), Gahai (salinity, 92 to 96 g/liter), Xiaochaidan (salinity, 94 to 99 g/liter), and Gasikule (salinity, 317 to 344 g/liter). The communities were dominated byBacteriain lakes with salinities of <100 g/liter and byArchaeain Lake Gasikule. The clades At12OctB3 andSalinibacter, previously reported only in hypersaline environments, were found in a hyposaline lake (salinity, 5.5 to 6.6 g/liter) at an abundance of ∼1.0%, indicating their ecological plasticity. Salinity and the concentrations of the chemical ions whose concentrations covary with salinity (Mg2+, K+, Cl−, Na+, SO42−, and Ca2+) were found to be the primary environmental factors that directly or indirectly determined the composition and diversity at the level of individual clades as well as entire prokaryotic communities. The distribution patterns of two phyla, five classes, five orders, five families, and three genera were well predicted by salinity. The variation of the prokaryotic community structure also significantly correlated with the dissolved oxygen concentration, pH, the total nitrogen concentration, and the PO43−concentration. Such correlations varied depending on the taxonomic level, demonstrating the importance of comprehensive correlation analyses at various taxonomic levels in evaluating the effects of environmental variable factors on prokaryotic community structures. Our findings clarify the distribution patterns of the prokaryotic community composition in plateau lakes at the levels of individual clades as well as whole communities along gradients of salinity and ionic concentrations.

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