Regulation of Phagotrophy by Prey, Low Nutrients, and Low Light in the Mixotrophic Haptophyte Isochrysis galbana

2021 ◽  
Author(s):  
Juan Manuel González-Olalla ◽  
Juan Manuel Medina-Sánchez ◽  
Alessandra Norici ◽  
Presentación Carrillo
Keyword(s):  
Isochrysis Galbana ◽  
Low Light

Extracellular carbohydrate liberation in the flagellates Isochrysis galbana and Prymnesium parvum

1965 ◽  
Vol 45 (3) ◽  
pp. 755-772 ◽  
Author(s):  
A. F. H. Marker
Keyword(s):  
Organic Matter ◽  
Light Intensity ◽  
Nitrogen Starvation ◽  
Axenic Culture ◽  
Isochrysis Galbana ◽  
Prymnesium Parvum ◽  
Extracellular Production ◽  
Low Light ◽  
Passive Release ◽  
Dying Cells

The production of extracellular carbohydrate has been studied in Isochrysis galbana and Prymnesium parvum in axenic culture. Increased extracellular production of carbohydrate occurred at reduced and increased salinity, low light intensity and under conditions of nitrogen starvation in Isochrysis, and in some cases appeared to be associated with the sedimentation of the cells from stagnant culture. Extracellular carbohydrate production was found to be greatest during the early and later stages in growth and dropped to a minimum during the mid-growth phase. Experiments indicated that the cells were not being damaged during harvesting of the cultures. A close similarity was found between the monosaccharide components of the intra- and extracellular carbohydrate after acid hydrolysis; both contained glucose, galactose, arabinose, xylose and ribose. It is suggested that the production of most of the extracellular carbohydrate is due to the passive release of organic matter from dead or dying cells.

Software pattern recognition applied to nanodiffraction patterns from a STEM instrument

1986 ◽  
Vol 44 ◽  
pp. 694-695
Author(s):  
G.Y. Fan ◽  
J.M. Cowley
Keyword(s):  
Image Processing ◽  
Pattern Recognition ◽  
Computer Software ◽  
Processing System ◽  
Light Level ◽  
Host Computer ◽  
Low Light ◽  
Image Processing System ◽  
Recent Developments ◽  
On Line

In recent developments, the ASU HB5 has been modified so that the timing, positioning, and scanning of the finely focused electron probe can be entirely controlled by a host computer. This made the asynchronized handshake possible between the HB5 STEM and the image processing system which consists of host computer (PDP 11/34), DeAnza image processor (IP 5000) which is interfaced with a low-light level TV camera, array processor (AP 400) and various peripheral devices. This greatly facilitates the pattern recognition technique initiated by Monosmith and Cowley. Software called NANHB5 is under development which, instead of employing a set of photo-diodes to detect strong spots on a TV screen, uses various software techniques including on-line fast Fourier transform (FFT) to recognize patterns of greater complexity, taking advantage of the sophistication of our image processing system and the flexibility of computer software.

Low-light-level three-dimensional video microscope with long working distance objective lenses

1994 ◽  
Vol 52 ◽  
pp. 226-227
Author(s):  
W. Lin ◽  
J. Gregorio ◽  
T.J. Holmes ◽  
D. H. Szarowski ◽  
J.N. Turner
Keyword(s):  
Three Dimensional ◽  
Light Level ◽  
Stereo Pair ◽  
Light Illumination ◽  
Initial Image ◽  
Low Light ◽  
Image Recording ◽  
Depth Discrimination ◽  
Video Microscope ◽  
Working Distance

A low-light level video microscope with long working distance objective lenses has been built as part of our integrated three-dimensional (3-D) light microscopy workstation (Fig. 1). It allows the observation of living specimens under sufficiently low light illumination that no significant photobleaching or alternation of specimen physiology is produced. The improved image quality, depth discrimination and 3-D reconstruction provides a versatile intermediate resolution system that replaces the commonly used dissection microscope for initial image recording and positioning of microelectrodes for neurobiology. A 3-D image is displayed on-line to guide the execution of complex experiments. An image composed of 40 optical sections requires 7 minutes to process and display a stereo pair.The low-light level video microscope utilizes long working distance objective lenses from Mitutoyo (10X, 0.28NA, 37 mm working distance; 20X, 0.42NA, 20 mm working distance; 50X, 0.42NA, 20 mm working distance). They provide enough working distance to allow the placement of microelectrodes in the specimen.

Morphogenetic machines revealed: Microscopy of living cells in the embryo of the frog, Xenopus laevis

1993 ◽  
Vol 51 ◽  
pp. 172-173
Author(s):  
Ray Keller
Keyword(s):  
Image Processing ◽  
Fluorescent Dyes ◽  
Cell Movement ◽  
Living Cells ◽  
Ease Of Use ◽  
Low Light ◽  
Amphibian Embryo ◽  
Large Populations ◽  
Light Fluorescence ◽  
Video Image Processing

The amphibian embryo offers advantages of size, availability, and ease of use with both microsurgical and molecular methods in the analysis of fundamental developmental and cell biological problems. However, conventional wisdom holds that the opacity of this embryo limits the use of methods in optical microscopy to resolve the cell motility underlying the major shape-generating processes in early development.These difficulties have been circumvented by refining and adapting several methods. First, methods of explanting and culturing tissues were developed that expose the deep, nonepithelial cells, as well as the superficial epithelial cells, to the view of the microscope. Second, low angle epi-illumination with video image processing and recording was used to follow patterns of cell movement in large populations of cells. Lastly, cells were labeled with vital, fluorescent dyes, and their behavior recorded, using low-light, fluorescence microscopy and image processing. Using these methods, the details of the cellular protrusive activity that drives the powerful convergence (narrowing)

Pendugaan Parameter Genetik Kedelai Generasi F4 pada Intensitas Cahaya Rendah

Agrotek ◽  
2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Yohanis Amos Mustamu ◽  
Trikoesoemaningtyas Trikoesoemaningtyas ◽  
Desta Wirnas ◽  
Didy Sopandie ◽  
Darman M. Arsyad
Keyword(s):  
Light Intensity ◽  
Genetic Parameter ◽  
Design Experiment ◽  
Low Light ◽  
Experimental Field ◽  
High Heritability ◽  
Intensity Condition ◽  
Augmented Design ◽  
Genetic Coefficient ◽  
The University

The objective of this study was to collect information on genetic parameter and agronomy character of soybean F4 generation in the low light intensity condition. The parameter was tested to 130 lines F4 which are produced by Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian (BBP2TP) Boor and the genotype of Sibayak, Tegal, Tanggamus, and Argomulyo were used as controls. The experiment was conducted in the university�s experimental field in Cikabayan, from September to December 2007. A total of 130 advance (F4) soybean lines were evaluated under shading in an augmented design experiment. The result of this study showed that all character has low genetic coefficient. The weight character of 25 grains has a considerably high heritability number in low li

The adaptation of Arctic phytoplankton to low light and salinity in Kongsfjorden (Spitsbergen)

2013 ◽  
Vol 23 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Shikai CUI ◽  
Jianfeng HE ◽  
Peimin HE ◽  
Fang ZHANG ◽  
Ling LIN ◽  
...  
Keyword(s):  
Low Light
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