Morphogenetic machines revealed: Microscopy of living cells in the embryo of the frog, Xenopus laevis

1993 ◽  
Vol 51 ◽  
pp. 172-173
Author(s):  
Ray Keller
Keyword(s):  
Image Processing ◽  
Fluorescent Dyes ◽  
Cell Movement ◽  
Living Cells ◽  
Ease Of Use ◽  
Low Light ◽  
Amphibian Embryo ◽  
Large Populations ◽  
Light Fluorescence ◽  
Video Image Processing

The amphibian embryo offers advantages of size, availability, and ease of use with both microsurgical and molecular methods in the analysis of fundamental developmental and cell biological problems. However, conventional wisdom holds that the opacity of this embryo limits the use of methods in optical microscopy to resolve the cell motility underlying the major shape-generating processes in early development.These difficulties have been circumvented by refining and adapting several methods. First, methods of explanting and culturing tissues were developed that expose the deep, nonepithelial cells, as well as the superficial epithelial cells, to the view of the microscope. Second, low angle epi-illumination with video image processing and recording was used to follow patterns of cell movement in large populations of cells. Lastly, cells were labeled with vital, fluorescent dyes, and their behavior recorded, using low-light, fluorescence microscopy and image processing. Using these methods, the details of the cellular protrusive activity that drives the powerful convergence (narrowing)

Recent advances in light microscopy

1994 ◽  
Vol 52 ◽  
pp. 388-389
Author(s):  
Brian Herman
Keyword(s):  
Fluorescent Dyes ◽  
Light Level ◽  
Optical Microscope ◽  
Living Cells ◽  
Low Light ◽  
Intact Cells ◽  
Time Observation ◽  
Single Living Cells ◽  
Single Living ◽  
Made In

The optical microscope has been an essential tool for generations of biologists, allowing the examination of details of individual cells and organisms impossible with the naked eye. The development of a large number of fluorescence dyes, allowing one to study the chemical and molecular dynamics of intact cells, has been of major importance in broadening the usefulness of optical microscopy. Attaching fluorescent dyes to specific molecular cell constituents allows real-time observation and quantitation of the activities of these constituents in cells and tissue. Beginning in the early 1950's, a number of improvements have been made in light microscope technology including advances in optics, digital computers, digitizers and image processing, and low-light-level (intensified) photodetectors. The ability of this instrumentation to intensify weakly fluorescent signals at the level of single living cells, without injuring the cell, has led to major advances in the understanding of the physiology and pathophysiology of living cells.

Software pattern recognition applied to nanodiffraction patterns from a STEM instrument

1986 ◽  
Vol 44 ◽  
pp. 694-695
Author(s):  
G.Y. Fan ◽  
J.M. Cowley
Keyword(s):  
Image Processing ◽  
Pattern Recognition ◽  
Computer Software ◽  
Processing System ◽  
Light Level ◽  
Host Computer ◽  
Low Light ◽  
Image Processing System ◽  
Recent Developments ◽  
On Line

In recent developments, the ASU HB5 has been modified so that the timing, positioning, and scanning of the finely focused electron probe can be entirely controlled by a host computer. This made the asynchronized handshake possible between the HB5 STEM and the image processing system which consists of host computer (PDP 11/34), DeAnza image processor (IP 5000) which is interfaced with a low-light level TV camera, array processor (AP 400) and various peripheral devices. This greatly facilitates the pattern recognition technique initiated by Monosmith and Cowley. Software called NANHB5 is under development which, instead of employing a set of photo-diodes to detect strong spots on a TV screen, uses various software techniques including on-line fast Fourier transform (FFT) to recognize patterns of greater complexity, taking advantage of the sophistication of our image processing system and the flexibility of computer software.

HVEM Analysis of Microfilament Patterns in The Margins of Tissue Cells

1980 ◽  
Vol 38 ◽  
pp. 808-811
Author(s):  
Carol Allen
Keyword(s):  
Cell Movement ◽  
Cell Spreading ◽  
Living Cells ◽  
Tissue Cell ◽  
Large Sample Size ◽  
Cell Periphery ◽  
Whole Cell ◽  
Critical Point Drying ◽  
Lamellar Region ◽  
Tissue Cells

When provided with a suitable solid substrate, tissue cells undergo a rapid conversion from the spherical form expressed in suspension culture to a characteristic flattened morphology. As a result of this conversion, called cell spreading, the cell nucleus and organelles come to occupy a central region of “deep cytoplasm” which slopes steeply into a peripheral “lamellar” region less than 1 pm thick at its outer edge and generally free of cell organelles. Cell spreading is accomplished by a continuous outward repositioning of the lamellar margins. Cell translocation on the substrate results when the activity of the lamellae on one side of the cell become dominant. When this occurs, the cell is “polarized” and moves in the direction of the “leading lamellae”. Careful analysis of tissue cell locomotion by time-lapse microphotography (1) has shown that the deformational movements of the leading lamellae occur in a repeating cycle of advance and retreat in the direction of cell movement and that the rate of such deformations are positively correlated with the speed of cell movement. In the present study, the physical basis for these movements of the cell margin has been examined by comparative light microscopy of living cells with whole-mount electron microscopy of fixed cells. Ultrastructural observations were made on tissue cells grown on Formvar-coated grids, fixed with glutaraldehyde, further processed by critical-point drying, and then photographed in the High Voltage Electron Microscope. This processing and imaging system maintains the 3-dimensional organization of the whole cell, the relationship of the cell to the substrate, and affords a large sample size which facilitates quantitative analysis. Comparative analysis of film records of living cells with the whole-cell micrographs revealed that specific patterns of microfilament organization consistently accompany recognizable stages of lamellar formation and movement. The margins of spreading cells and the leading lamellae of locomoting cells showed a similar pattern of MF repositionings (Figs. 1-4). These results will be discussed in terms of a working model for the mechanics of lamellar motility which includes the following major features: (a) lamellar protrusion results when an intracellular force is exerted at a locally weak area of the cell periphery; (b) the association of cortical MFs with one another determines the local resistance to this force; (c) where MF-to-MF association is weak, the cell periphery expands and some cortical MFs are dragged passively forward; (d) contact of the expanded area with the substrate then triggers the lateral association and reorientation of these cortical MFs into MF bundles parallel to the direction of the expansion; and (e) an active interaction between these MF bundles associated with the cortex of the expanded lamellae and the cortical MFs which remained in the sub-lamellar region then pulls the latter MFs forward toward the expanded area. Thus, the advance of the cell periphery on the substrate occurs in two stages: a passive phase in which some cortical MFs are dragged outward by the force acting to expand the cell periphery, and an active phase in which additional cortical MFs are pulled forward by interaction with the first set. Subsequent interactions between peripheral microfilament bundles and filaments in the deeper cytoplasm could then transmit the advance gained by lamellar expansion to the bulk of the cytoplasm.

Traffic incident detection system based on video image processing

2008 ◽  
Vol 28 (7) ◽  
pp. 1886-1889 ◽  
Author(s):  
Qin WANG ◽  
Shan HUANG ◽  
Hong-bin ZHANG ◽  
Quan YANG ◽  
Jian-jun ZHANG
Keyword(s):  
Image Processing ◽  
Detection System ◽  
Video Image ◽  
Incident Detection ◽  
Traffic Incident ◽  
Video Image Processing

scholarly journals Application of digital image processing in slope surface runoff velocity analysis under simulated rainfall conditions

2017 ◽  
Vol 49 (4) ◽  
pp. 1304-1312
Author(s):  
Tiexiong Gong ◽  
Yuanjun Zhu
Keyword(s):  
Image Processing ◽  
Surface Runoff ◽  
Lateral Diffusion ◽  
Ease Of Use ◽  
Velocity Analysis ◽  
Slope Gradient ◽  
Tracer Method ◽  
Dye Tracer ◽  
The Mean ◽  
High Runoff

Abstract To have accurate runoff velocity, there is need to improve dye tracer method for estimating surface runoff velocity. This can enhance the calculations of relevant hydrologic parameters that will lead to a better understanding of hydrological processes and soil erosion. In this study, an integrated dye tracer and image processing method (IPV) and dye tracer method (AOV), respectively, were used to estimate runoff velocity under three slope gradients (5°, 10°, and 15°) and three slope positions (up-slope, mid-slope, and down-slope). The results showed more variation in runoff velocity under IPV than AOV. Both IPV and AOV were positively correlated with slope gradient. IPV values were close to AOV ones for slope gradients ≤5°, but were significantly different for slope gradients ≥10°. The mean AOV value was 10.6% higher than that of IPV. Regression analysis showed that compared with AOV, IPV overestimated and underestimated runoff under low and high runoff velocity conditions, respectively. The use of image processing in IPV was advantageous because of its ease of use with fewer artificial errors and its suitability for lateral diffusion of runoff. Irrespectively, additional studies are needed to verify and/or improve further the use of this method in runoff velocity analysis.

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