Biodegradation of phenanthrene by Pseudomonas sp. strain PP2: novel metabolic pathway, role of biosurfactant and cell surface hydrophobicity in hydrocarbon assimilation

2003 ◽  
Vol 61 (4) ◽  
pp. 342-351 ◽  
Author(s):  
Y. Prabhu ◽  
P. S. Phale
Keyword(s):  
Cell Surface ◽  
Metabolic Pathway ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Pseudomonas Sp

The role of an extracellular polysaccharide produced by the marine Pseudomonas sp. S9 in cellular detachment during starvation

1989 ◽  
Vol 35 (2) ◽  
pp. 309-312 ◽  
Author(s):  
Michael Wrangstadh ◽  
Patricia L. Conway ◽  
Staffan Kjelleberg
Keyword(s):  
Cell Surface ◽  
Immunofluorescence Microscopy ◽  
Cell Surface Hydrophobicity ◽  
Hydrophobic Surface ◽  
Extracellular Polysaccharide ◽  
Surface Hydrophobicity ◽  
Bulk Phase ◽  
Bacterial Cells ◽  
Pseudomonas Sp

An exopolysaccharide polymer is produced by the marine Pseudomonas sp. S9 in response to complete energy and nutrient starvation. The presence of this polysaccharide on the cell surface and its subsequent release have been shown to be associated with both adhesion and detachment of the bacterial cells. Detachment from a hydrophobic surface was correlated to the presence of the exopolysaccharide on detached S9 cells. The exopolysaccharide was detected, using immunofluorescence microscopy, on surface-bound cells after only 15 min of exogenous energy and nutrient deprivation. This technique did not reveal any significant amounts of exopolysaccharide on starving bulk phase cells prior to 3 h of starvation. Cells that detached after 5.5 h of starvation had low cell surface hydrophobicity values and increased amounts of cell-bound exopolysaccharide. In contrast, cells that became detached during the first 5.5 h of starvation showed increasing hydrophobicity values during prolonged bulk phase starvation.

Role of lactobacillus cell surface hydrophobicity as probed by AFM in adhesion to surfaces at low and high ionic strength

2005 ◽  
Vol 41 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Virginia Vadillo-Rodríguez ◽  
Henk J. Busscher ◽  
Henny C. van der Mei ◽  
Joop de Vries ◽  
Willem Norde
Keyword(s):  
Ionic Strength ◽  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
High Ionic Strength

scholarly journals Factors associated with adherence to and biofilm formation on polystyrene byStenotrophomonas maltophilia: the role of cell surface hydrophobicity and motility

2008 ◽  
Vol 287 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Arianna Pompilio ◽  
Raffaele Piccolomini ◽  
Carla Picciani ◽  
Domenico D'Antonio ◽  
Vincenzo Savini ◽  
...  
Keyword(s):  
Cell Surface ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Factors Associated

scholarly journals Role of Cell Surface Hydrophobicity in the Pathogenesis of Medically-Significant Fungi

2021 ◽  
Vol 10 ◽  
Author(s):  
Carina Danchik ◽  
Arturo Casadevall
Keyword(s):  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Pathogenic Fungi ◽  
Surface Hydrophobicity ◽  
Dimorphic Fungi ◽  
Biophysical Parameter ◽  
Filamentous Cell ◽  
Cell Surface Interactions ◽  
Mold And Yeast

Cell surface hydrophobicity (CSH) is an important cellular biophysical parameter which affects both cell-cell and cell-surface interactions. In dimorphic fungi, multiple factors including the temperature-induced shift between mold and yeast forms have strong effects on CSH with higher hydrophobicity more common at the lower temperatures conducive to filamentous cell growth. Some strains of Cryptococcus neoformans exhibit high CSH despite the presence of the hydrophilic capsule. Among individual yeast colonies from the same isolate, distinct morphologies can correspond to differences in CSH. These differences in CSH are frequently associated with altered virulence in medically-significant fungi and can impact the efficacy of antifungal therapies. The mechanisms for the maintenance of CSH in pathogenic fungi remain poorly understood, but an appreciation of this fundamental cellular parameter is important for understanding its contributions to such phenomena as biofilm formation and virulence.

Role of cell surface hydrophobicity in Candida albicans biofilm

2013 ◽  
Vol 8 (3) ◽  
pp. 259-262 ◽  
Author(s):  
Helena Bujdáková ◽  
Miroslava Didiášová ◽  
Hana Drahovská ◽  
Lucia Černáková
Keyword(s):  
Gene Expression ◽  
Candida Albicans ◽  
Cell Surface ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Quantitative Real Time Pcr ◽  
Planktonic Cells ◽  
Planktonic Culture

AbstractOverall cell surface hydrophobicity (CSH) is predicted to play an important role during biofilm formation in Candida albicans but is the result of many expressed proteins. This study compares the CSH status and CSH1 gene expression in C. albicans planktonic cells, sessile biofilm, and dispersal cells. Greater percentages of hydrophobic cells were found in non-adhered (1.5 h) and dispersal forms (24 or 48 h) (41.34±4.17% and 39.52±7.45%, respectively), compared with overnight planktonic cultures (21.69±3.60%). Results from quantitative real-time PCR confirmed greater up-regulation of the CSH1 gene in sessile biofilm compared with both planktonic culture and dispersal cells. Up-regulation was also greater in dispersal cells compared with planktonic culture. The markedly increased CSH found both in C. albicans biofilm, and in cells released during biofilm formation could provide an advantage to dispersing cells building new biofilm.

scholarly journals UafB is a serine-rich repeat adhesin of Staphylococcus saprophyticus that mediates binding to fibronectin, fibrinogen and human uroepithelial cells

Microbiology ◽  
2011 ◽  
Vol 157 (4) ◽  
pp. 1161-1175 ◽  
Author(s):  
Nathan P. King ◽  
Scott A. Beatson ◽  
Makrina Totsika ◽  
Glen C. Ulett ◽  
Richard A. Alm ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Cell Surface ◽  
Molecular Mechanisms ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Factor B ◽  
Staphylococcus Saprophyticus ◽  
Uroepithelial Cells ◽  
Encoding Gene

Staphylococcus saprophyticus is an important cause of urinary tract infection (UTI), particularly among young women, and is second only to uropathogenic Escherichia coli as the most frequent cause of UTI. The molecular mechanisms of urinary tract colonization by S. saprophyticus remain poorly understood. We have identified a novel 6.84 kb plasmid-located adhesin-encoding gene in S. saprophyticus strain MS1146 which we have termed uro-adherence factor B (uafB). UafB is a glycosylated serine-rich repeat protein that is expressed on the surface of S. saprophyticus MS1146. UafB also functions as a major cell surface hydrophobicity factor. To characterize the role of UafB we generated an isogenic uafB mutant in S. saprophyticus MS1146 by interruption with a group II intron. The uafB mutant had a significantly reduced ability to bind to fibronectin and fibrinogen. Furthermore, we show that a recombinant protein containing the putative binding domain of UafB binds specifically to fibronectin and fibrinogen. UafB was not involved in adhesion in a mouse model of UTI; however, we observed a striking UafB-mediated adhesion phenotype to human uroepithelial cells. We have also identified genes homologous to uafB in other staphylococci which, like uafB, appear to be located on transposable elements. Thus, our data indicate that UafB is a novel adhesin of S. saprophyticus that contributes to cell surface hydrophobicity, mediates adhesion to fibronectin and fibrinogen, and exhibits tropism for human uroepithelial cells.

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