scholarly journals UafB is a serine-rich repeat adhesin of Staphylococcus saprophyticus that mediates binding to fibronectin, fibrinogen and human uroepithelial cells

Microbiology ◽  
2011 ◽  
Vol 157 (4) ◽  
pp. 1161-1175 ◽  
Author(s):  
Nathan P. King ◽  
Scott A. Beatson ◽  
Makrina Totsika ◽  
Glen C. Ulett ◽  
Richard A. Alm ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Cell Surface ◽  
Molecular Mechanisms ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Factor B ◽  
Staphylococcus Saprophyticus ◽  
Uroepithelial Cells ◽  
Encoding Gene

Staphylococcus saprophyticus is an important cause of urinary tract infection (UTI), particularly among young women, and is second only to uropathogenic Escherichia coli as the most frequent cause of UTI. The molecular mechanisms of urinary tract colonization by S. saprophyticus remain poorly understood. We have identified a novel 6.84 kb plasmid-located adhesin-encoding gene in S. saprophyticus strain MS1146 which we have termed uro-adherence factor B (uafB). UafB is a glycosylated serine-rich repeat protein that is expressed on the surface of S. saprophyticus MS1146. UafB also functions as a major cell surface hydrophobicity factor. To characterize the role of UafB we generated an isogenic uafB mutant in S. saprophyticus MS1146 by interruption with a group II intron. The uafB mutant had a significantly reduced ability to bind to fibronectin and fibrinogen. Furthermore, we show that a recombinant protein containing the putative binding domain of UafB binds specifically to fibronectin and fibrinogen. UafB was not involved in adhesion in a mouse model of UTI; however, we observed a striking UafB-mediated adhesion phenotype to human uroepithelial cells. We have also identified genes homologous to uafB in other staphylococci which, like uafB, appear to be located on transposable elements. Thus, our data indicate that UafB is a novel adhesin of S. saprophyticus that contributes to cell surface hydrophobicity, mediates adhesion to fibronectin and fibrinogen, and exhibits tropism for human uroepithelial cells.

Role of lactobacillus cell surface hydrophobicity as probed by AFM in adhesion to surfaces at low and high ionic strength

2005 ◽  
Vol 41 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Virginia Vadillo-Rodríguez ◽  
Henk J. Busscher ◽  
Henny C. van der Mei ◽  
Joop de Vries ◽  
Willem Norde
Keyword(s):  
Ionic Strength ◽  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
High Ionic Strength

scholarly journals Factors associated with adherence to and biofilm formation on polystyrene byStenotrophomonas maltophilia: the role of cell surface hydrophobicity and motility

2008 ◽  
Vol 287 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Arianna Pompilio ◽  
Raffaele Piccolomini ◽  
Carla Picciani ◽  
Domenico D'Antonio ◽  
Vincenzo Savini ◽  
...  
Keyword(s):  
Cell Surface ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Factors Associated

scholarly journals An Approach to Uropathogenic Escherichia Coli in Urinary Tract Infections

2010 ◽  
Vol 2 (02) ◽  
pp. 070-073 ◽  
Author(s):  
K Prabhat Ranjan ◽  
Neelima Ranjan ◽  
Arindam Chakraborty ◽  
D R Arora
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Cell Surface ◽  
Urinary Tract Infections ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Uropathogenic Escherichia Coli ◽  
Virulence Markers ◽  
The Difference ◽  
Tract Infections

ABSTRACT Purpose: To study the occurrence and characterization of Uropathogenic Escherichia coli (UPEC) in cases with urinary tract infections. Materials and Methods: A total of 220 symptomatic cases from urinary tract infections and 50 stool samples from apparently healthy individuals were included. The colonies identified as Escherichia coli were screened for virulence factors, that is, hemolysin, Mannose Resistant and Mannose Sensitive Hemagglutination (MRHA, MSHA), Cell surface hydrophobicity, and Serum resistance. Results: Among the 220 cases 91 (41.36%) were hemolytic, 68 (30.90%) showed MRHA, 58 (26.36%) were cell surface hydrophobicity positive, and 72 (32.72%) were serum-resistant. In 50 controls, three (6%) were hemolytic, six (12%) showed MRHA, nine (18%) showed cell surface hydrophobicity, and 12 (24%) were serum-resistant. The difference between cases and controls for hemolysis and MRHA were significant (P<0.001 and P<0.01, respectively). A total of 14 atypical E. coli were isolated from the urine and all showed the presence of one or the other virulence markers. Out of the 18 mucoid E.coli isolated, 10 were serum-resistant. Conclusions: The present study revealed that out of 220 urinary isolates, 151 could be labeled as UPEC.

scholarly journals Role of Cell Surface Hydrophobicity in the Pathogenesis of Medically-Significant Fungi

2021 ◽  
Vol 10 ◽  
Author(s):  
Carina Danchik ◽  
Arturo Casadevall
Keyword(s):  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Pathogenic Fungi ◽  
Surface Hydrophobicity ◽  
Dimorphic Fungi ◽  
Biophysical Parameter ◽  
Filamentous Cell ◽  
Cell Surface Interactions ◽  
Mold And Yeast

Cell surface hydrophobicity (CSH) is an important cellular biophysical parameter which affects both cell-cell and cell-surface interactions. In dimorphic fungi, multiple factors including the temperature-induced shift between mold and yeast forms have strong effects on CSH with higher hydrophobicity more common at the lower temperatures conducive to filamentous cell growth. Some strains of Cryptococcus neoformans exhibit high CSH despite the presence of the hydrophilic capsule. Among individual yeast colonies from the same isolate, distinct morphologies can correspond to differences in CSH. These differences in CSH are frequently associated with altered virulence in medically-significant fungi and can impact the efficacy of antifungal therapies. The mechanisms for the maintenance of CSH in pathogenic fungi remain poorly understood, but an appreciation of this fundamental cellular parameter is important for understanding its contributions to such phenomena as biofilm formation and virulence.

Role of cell surface hydrophobicity in Candida albicans biofilm

2013 ◽  
Vol 8 (3) ◽  
pp. 259-262 ◽  
Author(s):  
Helena Bujdáková ◽  
Miroslava Didiášová ◽  
Hana Drahovská ◽  
Lucia Černáková
Keyword(s):  
Gene Expression ◽  
Candida Albicans ◽  
Cell Surface ◽  
Biofilm Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Quantitative Real Time Pcr ◽  
Planktonic Cells ◽  
Planktonic Culture

AbstractOverall cell surface hydrophobicity (CSH) is predicted to play an important role during biofilm formation in Candida albicans but is the result of many expressed proteins. This study compares the CSH status and CSH1 gene expression in C. albicans planktonic cells, sessile biofilm, and dispersal cells. Greater percentages of hydrophobic cells were found in non-adhered (1.5 h) and dispersal forms (24 or 48 h) (41.34±4.17% and 39.52±7.45%, respectively), compared with overnight planktonic cultures (21.69±3.60%). Results from quantitative real-time PCR confirmed greater up-regulation of the CSH1 gene in sessile biofilm compared with both planktonic culture and dispersal cells. Up-regulation was also greater in dispersal cells compared with planktonic culture. The markedly increased CSH found both in C. albicans biofilm, and in cells released during biofilm formation could provide an advantage to dispersing cells building new biofilm.

scholarly journals A study of virulence factors of E. coli strains isolated from cases of urinary tract infection

2021 ◽  
Vol 8 (2) ◽  
pp. 163-167
Author(s):  
Sanjeev Dimri ◽  
Krati Varshney
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Cell Surface ◽  
Virulence Factors ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Clinical Microbiology Laboratory ◽  
E Coli ◽  
Tract Infections

The aim of this study was to demonstrate virulence factors namely adhesins, hemolysin and cell surface hydrophobicity of E. coli strains isolated from cases of urinary tract infection.A total of 210 E. coli strains were isolated from 602 culture positive cases of urinary tract infections. UPEC strains were screened for virulence factors namely hemolysin, hemagglutination and cell surface hydrophobicity by recommended methods. Out of 210 E. coli strains tested, 70 (33.34%) were hemolytic, 94 (44.76%) showed hemagglutination and 46 (21.90%) were cell surface hydrophobicity positive. Our study shows that a large number of uropathogenic E. coli strains had one or more virulence factors. The methods of detection of above-mentioned virulence factors are reasonably easy and screening them in a clinical microbiology laboratory is a worthwhile exercise.

scholarly journals Cell-surface hydrophobicity ofStaphylococcus saprophyticus

1991 ◽  
Vol 106 (1) ◽  
pp. 71-75 ◽  
Author(s):  
P. F. Schneider ◽  
T. V. Riley
Keyword(s):  
General Population ◽  
Cell Surface ◽  
Clinical Significance ◽  
Virulence Factor ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Coagulase Negative Staphylococci ◽  
Two Phase ◽  
Staphylococcus Saprophyticus ◽  
Urinary Isolates

SUMMARYThe cell-surface hydrophobicity of 100 urinary isolates ofStaphylococcus saprophyticus, cultured from symptomatic females in the general population, was assessed using a two-phase aqueous: hydrocarbon system. Relatively strong cell-surface hydrophobicity was exhibited by 79 isolates using the criteria employed, while only 2 of the remaining 21 isolates failed to demonstrate any detectable hydrophobicity. Cell-surface hydrophobicity may be a virulence factor ofS. saprophyticus. important in adherence of the organism to uroepithelia. Additionally, the data support the concept that cell-surface hydrophobicity may be a useful predictor of clinical significance of coagulase-negative staphylococci isoated from clinical sources.

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