Evaluation of soil bacterial biomass using environmental DNA extracted by slow-stirring method

2006 ◽  
Vol 71 (6) ◽  
pp. 875-880 ◽  
Author(s):  
H. Aoshima ◽  
A. Kimura ◽  
A. Shibutani ◽  
C. Okada ◽  
Y. Matsumiya ◽  
...  
Keyword(s):  
Bacterial Biomass ◽  
Environmental Dna ◽  
Soil Bacterial

scholarly journals Soil bacterial biomass and diversity are affected by different furrow-ridge mulched management systems during potato continuous cropping

2018 ◽  
Author(s):  
Weina Zhang ◽  
Shuhao Qin ◽  
Xuexue Xu ◽  
Junlian Zhang ◽  
Yuhui Liu
Keyword(s):  
Physicochemical Properties ◽  
Cropping Systems ◽  
Bacterial Biomass ◽  
The Other ◽  
Management Systems ◽  
Economic Losses ◽  
Continuous Cropping ◽  
Soil Physicochemical Properties ◽  
Bacterial Composition ◽  
Soil Bacterial

AbstractThe soil bacterial composition is vital for sustainable agriculture due to its importance in biogeochemical processes in the soil environment. Multiple management systems, such as different furrow-ridge mulched cropping systems, have been established to reduce the damage caused by continuous cropping of potato (Solanum tuberosumL.). However, little is known about the responses of soil bacterial biomass and diversity to these systems. In this study, six different ridge-furrow film planting patterns were tested in a 2-year continuous cropping potato field: flat plot without mulch (CK), flat plot with mulch (T1), on-ridge planting with full mulch (T2), on-furrow planting with full mulch (T3), on-ridge planting with half mulch (T4), and on-furrow planting with half mulch (T5). The soil physicochemical properties and bacterial composition were significantly affected by the planting pattern. Mulched soils, especially T2, maintained better soil physicochemical properties than controls. Fully mulched soil maintained higher bacterial biomass and diversity. Among the dominant genera, the abundances ofNitrosomonadaceaein T2 and T4 were higher than those in the other treatments. Consequently, compared with the other treatments, on-ridge with mulching patterns resulted in better soil physicochemical properties and high bacterial biomass and diversity, which could reduce the economic losses due to potato production by continuous cropping.

scholarly journals Resistance, Resilience, and Recovery of Dryland Soil Bacterial Communities Across Multiple Disturbances

2021 ◽  
Vol 12 ◽  
Author(s):  
Blaire Steven ◽  
Michala L. Phillips ◽  
Jayne Belnap ◽  
La Verne Gallegos-Graves ◽  
Cheryl R. Kuske ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Soil Structure ◽  
Bacterial Biomass ◽  
Natural Resistance ◽  
Rrna Gene ◽  
Community Recovery ◽  
Soil Bacterial Communities ◽  
Dryland Ecosystems ◽  
Soil Bacterial ◽  
And Function

Dryland ecosystems are sensitive to perturbations and generally slow to recover post disturbance. The microorganisms residing in dryland soils are especially important as they contribute to soil structure and nutrient cycling. Disturbance can have particularly strong effects on dryland soil structure and function, yet the natural resistance and recovery of the microbial components of dryland soils has not been well documented. In this study, the recovery of surface soil bacterial communities from multiple physical and environmental disturbances is assessed. Samples were collected from three field sites in the vicinity of Moab, UT, United States, 6 to 7 years after physical and climate disturbance manipulations had been terminated, allowing for the assessment of community recovery. Additionally, samples were collected in a transect that included three habitat patches: the canopy zone soils under the dominant shrubs, the interspace soils that are colonized by biological soil crusts, and edge soils at the plot borders. Field site and habitat patch were significant factors structuring the bacterial communities, illustrating that sites and habitats harbored unique soil microbiomes. Across the different sites and disturbance treatments, there was evidence of significant bacterial community recovery, as bacterial biomass and diversity were not significantly different than control plots. There was, however, a small number of 16S rRNA gene amplicon sequence variants that distinguished particular treatments, suggesting that legacy effects of the disturbances still remained. Taken together, these data suggest that dryland bacterial communities may possess a previously unappreciated potential to recover within years of the original disturbance.

Contribution of large bacteria to bacterial biomass in a deep freshwater lake (Lake Biwa, Japan)

2020 ◽  
Vol 85 ◽  
pp. 131-139
Author(s):  
S Shen ◽  
Y Shimizu
Keyword(s):  
Cell Volume ◽  
Bacterial Cell ◽  
Lake Biwa ◽  
Bacterial Biomass ◽  
Spatial Variations ◽  
Freshwater Lake ◽  
Heterotrophic Nanoflagellates ◽  
Bacterial Productivity ◽  
Transmission Electron ◽  
Seasonal And Spatial Variations

Despite the importance of bacterial cell volume in microbial ecology in aquatic environments, literature regarding the effects of seasonal and spatial variations on bacterial cell volume remains scarce. We used transmission electron microscopy to examine seasonal and spatial variations in bacterial cell size for 18 mo in 2 layers (epilimnion 0.5 m and hypolimnion 60 m) of Lake Biwa, Japan, a large and deep freshwater lake. During the stratified period, we found that the bacterial cell volume in the hypolimnion ranged from 0.017 to 0.12 µm3 (median), whereas that in the epilimnion was less variable (0.016 to 0.033 µm3, median) and much lower than that in the hypolimnion. Additionally, in the hypolimnion, cell volume during the stratified period was greater than that during the mixing period (up to 5.7-fold). These differences in cell volume resulted in comparable bacterial biomass in the hypolimnion and epilimnion, despite the fact that there was lower bacterial abundance in the hypolimnion than in the epilimnion. We also found that the biomass of larger bacteria, which are not likely to be grazed by heterotrophic nanoflagellates, increased in the hypolimnion during the stratified period. Our data suggest that estimation of carbon flux (e.g. bacterial productivity) needs to be interpreted cautiously when cell volume is used as a constant parametric value. In deep freshwater lakes, a difference in cell volume with seasonal and spatial variation may largely affect estimations.

Removal of Chromium (VI) From Leachate Using Bacterial Biomass

2013 ◽  
Vol 11 (11) ◽  
pp. 63-65 ◽  
Author(s):  
Ramesh Pun ◽  
Prakash Raut ◽  
Boj Raj Pant
Keyword(s):  
Bacterial Biomass ◽  
Chromium Vi ◽  
Scientific World

Scientific World, Vol. 11, No. 11, July 2013, page 63-65 DOI: http://dx.doi.org/10.3126/sw.v11i11.8554

The application of environmental DNA in the monitoring of the Yangtze finless porpoise, Neophocaena phocaenoides asaeorientalis

2019 ◽  
Vol 26 (1) ◽  
pp. 124
Author(s):  
Yunsheng WU ◽  
Yongkai TANG ◽  
Jianlin LI ◽  
Kai LIU ◽  
Hongxia LI ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Finless Porpoise ◽  
Yangtze Finless Porpoise ◽  
Neophocaena Phocaenoides

A flux-based stoichiometric model of enhanced biological phosphorus removal metabolism

1998 ◽  
Vol 37 (4-5) ◽  
pp. 609-613
Author(s):  
J. Pramanik ◽  
P. L. Trelstad ◽  
J. D. Keasling
Keyword(s):  
Phosphorus Removal ◽  
Reaction Network ◽  
Bacterial Biomass ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Stoichiometric Model ◽  
Aerobic Reactor ◽  
Complete Set ◽  
Biological Phosphorus

Enhanced biological phosphorus removal (EBPR) in wastewater treatment involves metabolic cycling through the biopolymers polyphosphate (polyP), polyhydroxybutyrate (PHB), and glycogen. This cycling is induced through treatment systems that alternate between carbon-rich anaerobic and carbon-poor aerobic reactor basins. While the appearance and disappearance of these biopolymers has been documented, the intracellular pressures that regulate their synthesis and degradation are not well understood. Current models of the EBPR process have examined a limited number of metabolic pathways that are frequently lumped into an even smaller number of “reactions.” This work, on the other hand, uses a stoichiometric model that contains a complete set of the pathways involved in bacterial biomass synthesis and energy production to examine EBPR metabolism. Using the stoichiometric model we were able to analyze the role of EBPR metabolism within the larger context of total cellular metabolism, as well as predict the flux distribution of carbon and energy fluxes throughout the total reaction network. The model was able to predict the consumption of PHB, the degradation of polyP, the uptake of acetate and the release of Pi. It demonstrated the relationship between acetate uptake and Pi release, and the effect of pH on this relationship. The model also allowed analysis of growth metabolism with respect to EBPR.

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