scholarly journals Behavioral response of dissimilatory perchlorate-reducing bacteria to different electron acceptors

2009 ◽  
Vol 84 (5) ◽  
pp. 955-963 ◽  
Author(s):  
Yvonne Sun ◽  
Ruth L. Gustavson ◽  
Nadia Ali ◽  
Karrie A. Weber ◽  
Lacey L. Westphal ◽  
...  
Keyword(s):  
Behavioral Response ◽  
Electron Acceptors ◽  
Reducing Bacteria

The influence of potassium dichromate on dissimilatory reduction of sulfate and nitrate ions by bacteria Desulfovibrio sp.

2017 ◽  
Vol 28 (1-2) ◽  
pp. 84-95
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
Ch. I. Bohoslavets ◽  
T. M. Hrytsun’ ◽  
B. M. Borsukevych
Keyword(s):  
Electron Acceptor ◽  
Potassium Dichromate ◽  
Anaerobic Respiration ◽  
Sulfate Reducing Bacteria ◽  
Negative Influence ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Nitrate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate reducing bacteria, capable to reductive transformation of different nature pollutants, used in biotechnologies of purification of sewage, contaminated by carbon, sulfur, nitrogen and metal compounds. H2S formed by them sediment metals to form of insoluble sulfides. Number of metals can be used by these microorganisms as electron acceptors during anaerobic respiration. Because under the influence of metal compounds observed slowing of bacteria metabolism, selection isolated from technologically modified ecotops resistant to pollutions strains is important task to create a new biotechnologies of purification. That’s why the purpose of this work was to study the influence of potassium dichromate, present in medium, on reduction of sulfate and nitrate ions by sulfate reducing bacteria Desulfovibrio desulfuricans IMV K-6, Desulfovibrio sp. Yav-6 and Desulfovibrio sp. Yav-8, isolated from Yavorivske Lake, to estimate the efficiency of possible usage of these bacteria in technologies of complex purification of environment from dangerous pollutants. Bacteria were cultivated in modified Kravtsov-Sorokin medium without SO42- and FeCl2×4H2O for 10 days. To study the influence of K2Cr2O7 on usage by bacteria SO42- or NO3- cells were seeded to media with Na2SO4×10H2O or NaNO3 and K2Cr2O7 at concentrations of 1.74 mM for total content of electron acceptors in medium 3.47 mM (concentration of SO42- in medium of standard composition). Cells were also seeded to media with 3.47 mM Na2SO4×10H2O, NaNO3 or K2Cr2O7 to investigate their growth in media with SO42-, NO3- or Cr2O72- as sole electron acceptor (control). Biomass was determined by turbidymetric method, content of sulfate, nitrate, dichromate, chromium (III) ions, hydrogen sulfide or ammonia ions in cultural liquid – by spectrophotometric method. It was found that K2Cr2O7 inhibits growth (2.2 and 1.3 times) and level of reduction by bacteria sulfate or nitrate ions (4.2 and 3.0 times, respectively) at simultaneous addition into cultivation medium of 1.74 mM SO42- or NO3- and 1.74 mM Cr2O72-, compared with growth and level of reduction of sulfate or nitrate ions in medium only with SO42- or NO3- as sole electron acceptor. Revealed that during cultivation of bacteria in presence of equimolar amount of SO42- or NO3- and Cr2O72-, last used by bacteria faster, content of Cr3+ during whole period of bacteria cultivation exceeded content H2S or NH4+. K2Cr2O7 in medium has most negative influence on dissimilatory reduction by bacteria SO42- than NO3-, since level of nitrate ions reduction by cells in medium with NO3- and Cr2O72- was a half times higher than level of sulfate ions reduction by it in medium with SO42- and Cr2O72-. The ability of bacteria Desulfovibrio sp. to priority reduction of Cr2O72- and after their exhaustion − NO3- and SO42- in the processes of anaerobic respiration can be used in technologies of complex purification of environment from toxic compounds.

scholarly journals Effect of abiotic factors on sulfidogenic activity of bacteria Desulfuromonas sp.

2020 ◽  
Vol 11 (2) ◽  
pp. 170-174
Author(s):  
O. M. Сhaіka ◽  
T. B. Peretyatko
Keyword(s):  
Hydrogen Sulfide ◽  
Cell Density ◽  
Elemental Sulfur ◽  
Abiotic Factors ◽  
Electron Acceptors ◽  
Stress Factors ◽  
Sulfide Concentration ◽  
Sulfur Concentration ◽  
Reducing Bacteria ◽  
Hydrogen Sulfide Concentration

Sulfur-reducing bacteria are promising agents for the development of new methods of wastewater treatment with the removal of ions of heavy metals and organic compounds. Study of the effect of various environmental factors on the growth and sulfidogenic activity of sulfur-reducing bacteria allows one to investigate the adaptability of these microorganisms to stress factors. The paper deals with the effect of рН, different concentrations of elemental sulfur, hydrogen sulfide and presence of various electron acceptors on the growth and sulfidogenic activity of bacteria Desulfuromonas sp. YSDS-3. The calculation of C/S ratio for sulfur-reducing bacteria Desulfuromonas sp. YSDS-3 was made, with the comparison with similar parameters of sulfate-reducing bacteria. In the medium with elemental sulfur, concentration of hydrogen sulfide increased with the concentration of elemental sulfur. Bacteria Desulfuromonas sp. YSDS-3 accumulated their biomass in the most effective way at the concentration of elemental sulfur of 10–100 mM. In the medium with polysulfide form of sulfur at the neutral pH, bacteria produced hydrogen sulfide and accumulated biomass the best. Hydrogen sulfide at the concentration of 3 mM did not inhibit the bacterial growth, but further increase in the hydrogen sulfide concentration inhibited the growth of bacteria. The bacteria did not grow at the hydrogen sulfide concentration of 25 mM and above. As the concentration of elemental sulfur and cell density increases, sulfidogenic activity of the bacteria grows. Presence of two electron acceptors (S and K2Cr2O7, S and MnO2, S and Fe (III)) did not affect the accumulation of biomass of the bacteria Desulfuromonas sp. YSDS-3. However, under such conditions the bacteria accumulated 1.5–2.5 times less hydrogen sulfide than in the test medium. After 12–24 h of cultivation, different concentrations of elemental sulfur had a significant effect on the sulfidogenic activity. However, during 3–16 days of cultivation, the percentage of effect of elemental sulfur concentration decreased to 31%, while the percentage of effect of cell density increased threefold. Presence in the medium of the electron acceptors (Cr (VI), MnO2, Fe (III)) alternative to elemental sulfur led to a significant decrease in the content of hydrogen sulfide produced by sulfur-reducing bacteria.

scholarly journals Usage of metals as the terminal electron acceptors by the sulfate-reducing bacteria

2009 ◽  
Vol 3 (3) ◽  
pp. 141-158 ◽  
Author(s):  
T. B. Peretyatko ◽  
◽  
A. A. Halushka ◽  
S. P. Gudz ◽  
◽  
...  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Electron Acceptors ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Reduction of azo dyes by desulfovibrio desulfuricans

2000 ◽  
Vol 41 (12) ◽  
pp. 15-22 ◽  
Author(s):  
E. S. Yoo ◽  
J. Libra ◽  
U. Wiesmann
Keyword(s):  
Azo Dyes ◽  
Desulfovibrio Desulfuricans ◽  
Chemical Processes ◽  
Electron Acceptors ◽  
Potential Toxicity ◽  
Sulfate Reducing ◽  
Textile Finishing ◽  
Reactive Orange ◽  
Reducing Bacteria ◽  
Electron Carriers

Azo dyes are widely used in textile finishing, and have become of concern in wastewater treatment because of their color, bio-recalcitrance, and potential toxicity to animals and humans. Thus, wastewater with azo dyes must be decolorized and furthermore mineralized in appropriate systems combining biological and chemical processes. In this study, the potential for sulfate reducing bacteria (SRB) to decolorize azo dyes was studied, employing the pure culture of Desulfovibrio desulfuricans (D. desulfuricans) with varying sulfate levels. Under sulfate-rich conditions, the sulfide produced from sulfate respiration with pyruvate (electron donor) by D. desulfuricans chemically decolorized the azo dyes C. I. Reactive Orange 96 (RO 96) and C. I. Reactive Red 120 (RR 120). Under sulfate-depleted conditions (≤0.1 mmol/L), the decolorization of RO 96 and RR 120 occurred in correlation with the fermentation of pyruvate by D. desulfuricans. It is suggested that the electrons liberated from the pyruvate oxidation were transferred via enzymes and/or coenzymes (electron carriers) to the dyes as alternative terminal electron acceptors, giving rise to decolorization, instead of to the protons (H+), resulting in the production of H2. Both decolorization pathways were compared in light of bioenergetics and engineering aspects.

scholarly journals Shewanella oneidensis MR-1 Restores Menaquinone Synthesis to a Menaquinone-Negative Mutant

2004 ◽  
Vol 70 (9) ◽  
pp. 5415-5425 ◽  
Author(s):  
Charles R. Myers ◽  
Judith M. Myers
Keyword(s):  
Shewanella Oneidensis ◽  
Electron Acceptors ◽  
Intensive Study ◽  
Feeding Experiments ◽  
Acid Analog ◽  
Previous Hypothesis ◽  
Redox Shuttle ◽  
Insoluble Electron Acceptors ◽  
Reducing Bacteria ◽  
Negative Mutant

ABSTRACT The mechanisms underlying the use of insoluble electron acceptors by metal-reducing bacteria, such as Shewanella oneidensis MR-1, are currently under intensive study. Current models for shuttling electrons across the outer membrane (OM) of MR-1 include roles for OM cytochromes and the possible excretion of a redox shuttle. While MR-1 is able to release a substance that restores the ability of a menaquinone (MK)-negative mutant, CMA-1, to reduce the humic acid analog anthraquinone-2,6-disulfonate (AQDS), cross-feeding experiments conducted here showed that the substance released by MR-1 restores the growth of CMA-1 on several soluble electron acceptors. Various strains derived from MR-1 also release this substance; these include mutants lacking the OM cytochromes OmcA and OmcB and the OM protein MtrB. Even though strains lacking OmcB and MtrB cannot reduce Fe(III) or AQDS, they still release a substance that restores the ability of CMA-1 to use MK-dependent electron acceptors, including AQDS and Fe(III). Quinone analysis showed that this released substance restores MK synthesis in CMA-1. This ability to restore MK synthesis in CMA-1 explains the cross-feeding results and challenges the previous hypothesis that this substance represents a redox shuttle that facilitates metal respiration.

scholarly journals Reduction of perchlorate ions by the sulfate-reducing bacteria Desulfotomaculum sp. and Desulfovibrio desulfuricans

2020 ◽  
Vol 11 (2) ◽  
pp. 278-282
Author(s):  
N. S. Verkholiak ◽  
T. B. Peretyatko ◽  
A. A. Halushka
Keyword(s):  
Sewage Treatment ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Inhibitory Effect ◽  
Electron Acceptors ◽  
Wood Chips ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Perchlorate Ion ◽  
Reducing Bacteria

The usage of microorganisms to clean the environment from xenobiotics, in particular chlorine-containing ones, is a promising method of detoxifying the contaminated environment. Sulfate-reducing bacteria Desulfovibrio desulfuricans Ya-11, isolated from Yavoriv Lake, and Desulfotomaculum AR1, isolated from the Lviv sewage treatment system, are able to grow under conditions of environmental contamination by aromatic compounds and chlorine-containing substances. Due to their high redox potential, chlorate and perchlorate ions can be ideal electron acceptors for the metabolism of microorganisms. To test the growth of the tested microorganisms under the influence of perchlorate ions, bacteria were cultured in modified Postgate C medium with ClO4–. Biomass was determined turbidimetrically, the content of sulfate ions and hydrogen sulfide – photoelectrocolorimetrically, the content of perchlorate ions – permanganatometrically. The study of the ability of sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 to grow in a medium with perchlorate ions as electron acceptors showed the inhibitory effect of ClO4– on sulfate ion reduction by bacteria. Bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to grow in environments with aromatic hydrocarbons, in particular toluene. The possibility of the growth of sulfate-reducing bacteria in the presence of toluene as an electron donor and perchlorate ions as an electron acceptor was investigated. The efficiency of perchlorate ion utilization by sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was about 90 %. The effect of molybdenum on the reduction of perchlorate ions by Desulfotomaculum AR1 is shown in the paper. Immobilization of bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was carried out in 3% agar and on wood chips. The ability of bacteria, immobilized on these media, to purify the aqueous medium from perchlorate ions was investigated. Reduction of perchlorate ions is more efficiently performed by cells of Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria immobilized in agar than on wood chips. Sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to use perchlorate ions as electron acceptors, purifying the polluted aquatic environment from these pollutants.

scholarly journals (Per)Chlorate-Reducing Bacteria Can Utilize Aerobic and Anaerobic Pathways of Aromatic Degradation with (Per)Chlorate as an Electron Acceptor

mBio ◽  
2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Charlotte I. Carlström ◽  
Dana Loutey ◽  
Stefan Bauer ◽  
Iain C. Clark ◽  
Robert A. Rohde ◽  
...  
Keyword(s):  
Aromatic Compound ◽  
Electron Acceptor ◽  
Electron Acceptors ◽  
Low Oxygen ◽  
Content Type ◽  
Reducing Conditions ◽  
Concurrent Use ◽  
Anoxic Environments ◽  
Aromatic Degradation ◽  
Reducing Bacteria

ABSTRACTThe pathways involved in aromatic compound oxidation under perchlorate and chlorate [collectively known as (per)chlorate]-reducing conditions are poorly understood. Previous studies suggest that these are oxygenase-dependent pathways involving O2biogenically produced during (per)chlorate respiration. Recently, we describedSedimenticola selenatireducensCUZ and Dechloromarinus chlorophilus NSS, which oxidized phenylacetate and benzoate, two key intermediates in aromatic compound catabolism, coupled to the reduction of perchlorate or chlorate, respectively, and nitrate. While strain CUZ also oxidized benzoate and phenylacetate with oxygen as an electron acceptor, strain NSS oxidized only the latter, even at a very low oxygen concentration (1%, vol/vol). Strains CUZ and NSS contain similar genes for both the anaerobic and aerobic-hybrid pathways of benzoate and phenylacetate degradation; however, the key genes (paaABCD) encoding the epoxidase of the aerobic-hybrid phenylacetate pathway were not found in either genome. By using transcriptomics and proteomics, as well as by monitoring metabolic intermediates, we investigated the utilization of the anaerobic and aerobic-hybrid pathways on different electron acceptors. For strain CUZ, the results indicated utilization of the anaerobic pathways with perchlorate and nitrate as electron acceptors and of the aerobic-hybrid pathways in the presence of oxygen. In contrast, proteomic results suggest that strain NSS may use a combination of the anaerobic and aerobic-hybrid pathways when growing on phenylacetate with chlorate. Though microbial (per)chlorate reduction produces molecular oxygen through the dismutation of chlorite (ClO2−), this study demonstrates that anaerobic pathways for the degradation of aromatics can still be utilized by these novel organisms.IMPORTANCES. selenatireducens CUZ and D. chlorophilus NSS are (per)chlorate- and chlorate-reducing bacteria, respectively, whose genomes encode both anaerobic and aerobic-hybrid pathways for the degradation of phenylacetate and benzoate. Previous studies have shown that (per)chlorate-reducing bacteria and chlorate-reducing bacteria (CRB) can use aerobic pathways to oxidize aromatic compounds in otherwise anoxic environments by capturing the oxygen produced from chlorite dismutation. In contrast, we demonstrate that S. selenatireducens CUZ is the first perchlorate reducer known to utilize anaerobic aromatic degradation pathways with perchlorate as an electron acceptor and that it does so in preference over the aerobic-hybrid pathways, regardless of any oxygen produced from chlorite dismutation. D. chlorophilus NSS, on the other hand, may be carrying out anaerobic and aerobic-hybrid processes simultaneously. Concurrent use of anaerobic and aerobic pathways has not been previously reported for other CRB or any microorganisms that encode similar pathways of phenylacetate or benzoate degradation and may be advantageous in low-oxygen environments.

scholarly journals Usage of ferrum (ІІІ) and manganese (IV) ions as electron acceptors by Desulfuromonas sp. bacteria

2016 ◽  
Vol 24 (1) ◽  
pp. 87-95 ◽  
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
C. I. Bohoslavets ◽  
G. V. Yavorska ◽  
N. V. Truchym
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Anaerobic Respiration ◽  
Biomass Accumulation ◽  
Culture Liquid ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Insignificant Difference ◽  
High Concentrations ◽  
Reducing Bacteria

The toxicity of metal ions to microorganisms, in particular at high concentrations, is one of the main impediments to their usage in remediation technologies. The purpose of this work is to analyze the possibility of usage by bacteria of the Desulfuromonas genus, isolated by us from Yavorivske Lake, of ferrum (ІІІ) and manganese (IV) ions at concentrations in the medium of 1,74–10,41 mM as electron acceptors of anaerobic respiration to assesss resistance of sulphur reducing bacteria strains to heavy metal compounds. Cells of Desulfuromonas acetoxidans ІМV V-7384, Desulfuromonas sp. Yavor-5 and Desulfuromonas sp. Yavor-7 were cultivated for 10 days at 30 °C under anaerobic conditions in Kravtsov-Sorokin’s medium without sulphate ions, sulphur, with cysteine as the sulphur source (0.2 g/l) and sodium lactate or citrate as the electron donor (17.86 g/l), in which were added sterile 1 M solutions of C6H5O7Fe and C4H4O4 (control) and also weights of MnO2 to their terminal concentrations 1.74, 3.47, 5.21, 6.94, 10.41 mM. Biomass was determined by the turbidimetric method. In the culture liquid the presence of Fe3+ and Mn4+ were qualitatively determined, and the content of Fe2+ in reaction with о-phenanthroline was determined quantitatively. It was established that sulphur reducing bacteria used with different intensity ferrum (ІІІ) and manganese (IV) ions as electron acceptors during the process of anaerobic respiration at concentrations of 1.74–10.41 mM C6H5O7Fe and MnO2 in the medium, which demonstrated the important role of the investigated microorganisms in reductive detoxication of natural and technogenic media from oxidized forms of transitional heavy metals. An insignificant difference in biomass accumulation during usage of 5.21–10.41 mM ferrum (ІІІ) ions and fumarate is caused by toxicity of the metal ions to cells since the high redox potential of the Fe(III)/Fe(ІІ) pair with increase in concentrations of electron acceptors in the medium did not lead to increase in the biomass accumulation level. The greatest biomass of the bacteria accumulated on the 8–10th days in the medium with the lowest concentration of C6H5O7Fe – 1.74 mM (up to 2.77 g/l), and the lowest biomass – with highest concentration – 10.41 mM (up to 2.41 g/l). After 10 days of cultivation the bacteria of all strains had fully used the ferrum (ІІІ) ions present in the medium. A biomass yield almost twice as low was revealed after manganese (IV) oxide was used by bacteria compared with its use of ferrum (ІІІ) citrate and fumarate at all studied concentrations of electron acceptors in the medium. The highest biomass of bacteria accumulated in the medium with the lowest MnO2 content – 1.74 mM (up to 1.35 g/l), and the lowest biomass in the medium with the highest content – 10.41 mM (up to 1.15 g/l). After 10 days of cultivation bacteria of all strains had not fully restored the manganese (IV) ions present in the medium. The greatest biomass compared with other strains after growth in medium with different C6H5O7Fe and MnO2 contents was accumulated by the strain Desulfuromonas sp. Yavor-7. Since sulphur reducing bacteria strains proved to be resistant to Fe3+ and Mn4+ high concentrations (up to10.41 mM) they can be successfully used in technologies of environmenal remediation from sulphur and heavy metal compounds. 

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