scholarly journals Usage of ferrum (ІІІ) and manganese (IV) ions as electron acceptors by Desulfuromonas sp. bacteria

2016 ◽  
Vol 24 (1) ◽  
pp. 87-95 ◽  
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
C. I. Bohoslavets ◽  
G. V. Yavorska ◽  
N. V. Truchym
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Anaerobic Respiration ◽  
Biomass Accumulation ◽  
Culture Liquid ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Insignificant Difference ◽  
High Concentrations ◽  
Reducing Bacteria

The toxicity of metal ions to microorganisms, in particular at high concentrations, is one of the main impediments to their usage in remediation technologies. The purpose of this work is to analyze the possibility of usage by bacteria of the Desulfuromonas genus, isolated by us from Yavorivske Lake, of ferrum (ІІІ) and manganese (IV) ions at concentrations in the medium of 1,74–10,41 mM as electron acceptors of anaerobic respiration to assesss resistance of sulphur reducing bacteria strains to heavy metal compounds. Cells of Desulfuromonas acetoxidans ІМV V-7384, Desulfuromonas sp. Yavor-5 and Desulfuromonas sp. Yavor-7 were cultivated for 10 days at 30 °C under anaerobic conditions in Kravtsov-Sorokin’s medium without sulphate ions, sulphur, with cysteine as the sulphur source (0.2 g/l) and sodium lactate or citrate as the electron donor (17.86 g/l), in which were added sterile 1 M solutions of C6H5O7Fe and C4H4O4 (control) and also weights of MnO2 to their terminal concentrations 1.74, 3.47, 5.21, 6.94, 10.41 mM. Biomass was determined by the turbidimetric method. In the culture liquid the presence of Fe3+ and Mn4+ were qualitatively determined, and the content of Fe2+ in reaction with о-phenanthroline was determined quantitatively. It was established that sulphur reducing bacteria used with different intensity ferrum (ІІІ) and manganese (IV) ions as electron acceptors during the process of anaerobic respiration at concentrations of 1.74–10.41 mM C6H5O7Fe and MnO2 in the medium, which demonstrated the important role of the investigated microorganisms in reductive detoxication of natural and technogenic media from oxidized forms of transitional heavy metals. An insignificant difference in biomass accumulation during usage of 5.21–10.41 mM ferrum (ІІІ) ions and fumarate is caused by toxicity of the metal ions to cells since the high redox potential of the Fe(III)/Fe(ІІ) pair with increase in concentrations of electron acceptors in the medium did not lead to increase in the biomass accumulation level. The greatest biomass of the bacteria accumulated on the 8–10th days in the medium with the lowest concentration of C6H5O7Fe – 1.74 mM (up to 2.77 g/l), and the lowest biomass – with highest concentration – 10.41 mM (up to 2.41 g/l). After 10 days of cultivation the bacteria of all strains had fully used the ferrum (ІІІ) ions present in the medium. A biomass yield almost twice as low was revealed after manganese (IV) oxide was used by bacteria compared with its use of ferrum (ІІІ) citrate and fumarate at all studied concentrations of electron acceptors in the medium. The highest biomass of bacteria accumulated in the medium with the lowest MnO2 content – 1.74 mM (up to 1.35 g/l), and the lowest biomass in the medium with the highest content – 10.41 mM (up to 1.15 g/l). After 10 days of cultivation bacteria of all strains had not fully restored the manganese (IV) ions present in the medium. The greatest biomass compared with other strains after growth in medium with different C6H5O7Fe and MnO2 contents was accumulated by the strain Desulfuromonas sp. Yavor-7. Since sulphur reducing bacteria strains proved to be resistant to Fe3+ and Mn4+ high concentrations (up to10.41 mM) they can be successfully used in technologies of environmenal remediation from sulphur and heavy metal compounds. 

The influence of potassium dichromate on dissimilatory reduction of sulfate and nitrate ions by bacteria Desulfovibrio sp.

2017 ◽  
Vol 28 (1-2) ◽  
pp. 84-95
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
Ch. I. Bohoslavets ◽  
T. M. Hrytsun’ ◽  
B. M. Borsukevych
Keyword(s):  
Electron Acceptor ◽  
Potassium Dichromate ◽  
Anaerobic Respiration ◽  
Sulfate Reducing Bacteria ◽  
Negative Influence ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Nitrate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate reducing bacteria, capable to reductive transformation of different nature pollutants, used in biotechnologies of purification of sewage, contaminated by carbon, sulfur, nitrogen and metal compounds. H2S formed by them sediment metals to form of insoluble sulfides. Number of metals can be used by these microorganisms as electron acceptors during anaerobic respiration. Because under the influence of metal compounds observed slowing of bacteria metabolism, selection isolated from technologically modified ecotops resistant to pollutions strains is important task to create a new biotechnologies of purification. That’s why the purpose of this work was to study the influence of potassium dichromate, present in medium, on reduction of sulfate and nitrate ions by sulfate reducing bacteria Desulfovibrio desulfuricans IMV K-6, Desulfovibrio sp. Yav-6 and Desulfovibrio sp. Yav-8, isolated from Yavorivske Lake, to estimate the efficiency of possible usage of these bacteria in technologies of complex purification of environment from dangerous pollutants. Bacteria were cultivated in modified Kravtsov-Sorokin medium without SO42- and FeCl2×4H2O for 10 days. To study the influence of K2Cr2O7 on usage by bacteria SO42- or NO3- cells were seeded to media with Na2SO4×10H2O or NaNO3 and K2Cr2O7 at concentrations of 1.74 mM for total content of electron acceptors in medium 3.47 mM (concentration of SO42- in medium of standard composition). Cells were also seeded to media with 3.47 mM Na2SO4×10H2O, NaNO3 or K2Cr2O7 to investigate their growth in media with SO42-, NO3- or Cr2O72- as sole electron acceptor (control). Biomass was determined by turbidymetric method, content of sulfate, nitrate, dichromate, chromium (III) ions, hydrogen sulfide or ammonia ions in cultural liquid – by spectrophotometric method. It was found that K2Cr2O7 inhibits growth (2.2 and 1.3 times) and level of reduction by bacteria sulfate or nitrate ions (4.2 and 3.0 times, respectively) at simultaneous addition into cultivation medium of 1.74 mM SO42- or NO3- and 1.74 mM Cr2O72-, compared with growth and level of reduction of sulfate or nitrate ions in medium only with SO42- or NO3- as sole electron acceptor. Revealed that during cultivation of bacteria in presence of equimolar amount of SO42- or NO3- and Cr2O72-, last used by bacteria faster, content of Cr3+ during whole period of bacteria cultivation exceeded content H2S or NH4+. K2Cr2O7 in medium has most negative influence on dissimilatory reduction by bacteria SO42- than NO3-, since level of nitrate ions reduction by cells in medium with NO3- and Cr2O72- was a half times higher than level of sulfate ions reduction by it in medium with SO42- and Cr2O72-. The ability of bacteria Desulfovibrio sp. to priority reduction of Cr2O72- and after their exhaustion − NO3- and SO42- in the processes of anaerobic respiration can be used in technologies of complex purification of environment from toxic compounds.

Facile one-pot synthesis of highly monodisperse nickel microspheres with raised nickel dots and their adsorption performance for heavy metal ions

2016 ◽  
Vol 45 (23) ◽  
pp. 9704-9711 ◽  
Author(s):  
Yue Teng ◽  
Le Xin Song ◽  
Wei Liu ◽  
Li Zhao ◽  
Juan Xia ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Solvothermal Synthesis ◽  
Heavy Metal Ions ◽  
One Pot ◽  
Adsorption Performance ◽  
One Pot Synthesis ◽  
High Concentrations

Monodispersed Ni microspheres with surfaces uniformly covered by Ni dots were constructed through a facile solvothermal synthesis, showing an improved ability to remove Cd2+ ions even at high concentrations in water.

scholarly journals Heavy Non-ferrous Metals Adsorption from Water Solutions with Granular Filtering Materials Based on Silicon Rock

2018 ◽  
Author(s):  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Ph Value ◽  
Salt Content ◽  
Human Beings ◽  
Waste Waters ◽  
Metal Compounds ◽  
Physical Chemical ◽  
Siliceous Rocks

Water factor plays an important role in formation of healthy environment for human beings. Due to unfavorable anthropogenic impact upon environment certain surface and groundwater water supply sources are contaminated with heavy metal compounds. Clints (siliceous rocks) are considered the most promising materials for the natural and waste waters treatment from such ingredients. The paper presents the results of the siliceous rocks’ physical/chemical and operational properties studying. It has been shown that these siliceous rocks satisfy all requirements to filtering materials. Methods of physical/chemical analysis were applied for studying the adsorption processes nature. It was for the first time stated (with methods of potentiometric titration and infra-red spectroscopy) that siliceous rocks included the fixed functional ion-exchange groups in their composition and were subacid cationits. Beside chemical adsorption heavy metal ions interact with siliceous rocks due to the action of their physical nature forces. Physical adsorption contributes the most in the heavy metal compounds up-taking by filtering materials, this is supported by the fact of low values of the process activation energy: from 3 to 8 kJ/gram-molecule. Parameters of the process of heavy metal ions adsorption with siliceous rocks in static and dynamic conditions have been calculated in accordance with the experimental results. Influence of salt content and the liquid phase pH value on the adsorption process has been stated. Optimal conditions for the worked siliceous rocks regeneration have been determined. New data on heavy metals cations adsorption with nuclear filtering materials confirm principal possibility of their use for natural and waste waters treatment.

PREPARATION AND PROPERTIES OF TRANSKETOLASE FROM PORK LIVER

1960 ◽  
Vol 38 (1) ◽  
pp. 115-124 ◽  
Author(s):  
F. J. Simpson
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Thiamine Pyrophosphate ◽  
Magnesium Ions ◽  
Optimum Activity ◽  
Pork Liver ◽  
High Concentrations ◽  
The Stability ◽  
Ph Range

Transketolase of pork liver has been purified 90-fold and separated from ribulose 5-phosphate 3-epimerase. The transketolase is most stable between pH 7.5 and 8.5 and below 40 °C. The pH range for optimum activity is between 7.6 and 8.1. Activation by magnesium ions or thiamine pyrophosphate could not be demonstrated, but thiamine pyrophosphate increased the stability of the enzyme. Sulphydryl agents, such as p-chloromercuriphenyl sulphonic acid and N-ethylmaleimide, and heavy metal ions, such as cupric, mercuric, and zinc, at relatively high concentrations inhibited the enzyme.

scholarly journals Secondary pollution of water during the biosorption of heavy metal ions by pristine and sugar beet shreds from bioethanol production

2018 ◽  
pp. 81-91 ◽  
Author(s):  
Dragana Kukic ◽  
Marina Sciban ◽  
Vesna Vasic ◽  
Jelena Prodanovic
Keyword(s):  
Heavy Metal ◽  
Sugar Beet ◽  
Metal Ions ◽  
Adsorption Capacity ◽  
Heavy Metal Ions ◽  
Bioethanol Production ◽  
Limit Values ◽  
Secondary Pollution ◽  
Emission Limit ◽  
High Concentrations

When biomaterials are used as adsorbents, some compounds present in their structure can be extracted and cause secondary pollution of the treated water. Depending on the type and amount of the secondary pollution, some materials can be unacceptable as adsorbents despite of their good adsorption capacity. Although, it is not a newly discovered phenomenon and it is recognized as a problem, leaching from adsorbents is neglected. The aim of our study is to investigate the secondary pollution of the water during the biosorption of heavy metal ions by pristine and modified sugar beet shreds from bioethanol production. The presence of different elements in the mentioned adsorbents, secondary pollution of the water during the adsorption of heavy metal ions and amount of reducing sugars in the water treated by pristine sugar beet shreds were characterized by the SEM-EDS method, the amount of the leached inorganic and organic matter from the adsorbents (atomic absorption spectroscopy and permanganate index) and Bertrand's method, respectively. Furthermore, the obtained results were compared to the available literature data. Our results showed that according to the valid regulations of emission limit values of the polluting substances in the water and adsorption capacity, the investigated adsorbents have a great potential as biosorbents for practical treatment of wastewaters which contain high concentrations of heavy metal ions.

scholarly journals Sulfidogenic activity of sulfate and sulfur reducing bacteria under the influence of metal compounds

2018 ◽  
Vol 26 (1) ◽  
pp. 3-10
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
O. V. Tarabas ◽  
C. I. Bohoslavets ◽  
G. V. Yavorska ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Hydrogen Sulfide ◽  
Metal Ions ◽  
Spectrophotometric Method ◽  
Biological Diversity ◽  
Natural Environments ◽  
Metal Compounds ◽  
Bacteria Growth ◽  
Production Of Hydrogen ◽  
Reducing Bacteria

Due to their high content in natural environments, heavy metals exhibit toxic effects on living organisms, which leads to a decrease in the biological diversity and productivity of ecosystems. In niches with low oxidation reducing potential, sulfate and sulfur reducing bacteria carry out the reducing transformation of oxidized sulfur compounds with the formation of significant amounts of hydrogen sulfide. H2S produced by bacteria interacts with metal ions, precipitating them in the form of sulfides. The aim of this work was to investigate the influence of lead, cuprum (II), iron (II) and manganese (II) salts on the production of hydrogen sulfide by bacteria of the Desulfovibrio and Desulfuromonas genera, isolated from Yavorivske Lake, and to evaluate the efficiency of their use for purifying media, enriched with organic compounds, from hydrogen sulfide and heavy metals. The content of heavy metal ions in the water of Yavorivske Lake was determined by the spectrophotometric method. The bacteria were grown for 10 days at 30 °C in the Kravtsov-Sorokin medium under anaerobic conditions. To study the influence of metal ions on bacteria growth and their H2S production, cells were incubated with metal salts (0.5–4.0 mM), washed and grown in media with SO42– or S0. To determine the level of metal ions binding by H2S, produced by bacteria, cells were grown in media with metal compounds (0.5–4.0 mM), SO42– or S0. Biomass was determined by turbidimetric method. In the cultural liquid the content of H2S was determined quantitatively by spectrophotometric method, and qualitatively by the presence of metal cations. The content of metal sulfides in the growth medium was determined by weight method. Sulfate and sulfur-reducing bacteria were resistant to 2.0 mM Pb(NO3)2, 2.5 mM CuCl2, 2.5 mM FeCl2 × 4H2O and 2.0 mM MnCl2 × 4H2O, therefore they are promising for the development of biotechnologies for the purification of water resources contaminated by sulfur and metal compounds. When present in a medium with sulfates or sulfur of 1.0–1.5 mM lead, cuprum (II), iron (II) or manganese (II) ions, they almost completely bind with the H2S produced by bacteria in the form of insoluble sulfides, which confirms the negative results of qualitative reactions to their presence in the cultural liquid.

PREPARATION AND PROPERTIES OF TRANSKETOLASE FROM PORK LIVER

1960 ◽  
Vol 38 (2) ◽  
pp. 115-124 ◽  
Author(s):  
F. J. Simpson
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Thiamine Pyrophosphate ◽  
Magnesium Ions ◽  
Optimum Activity ◽  
Pork Liver ◽  
High Concentrations ◽  
The Stability ◽  
Ph Range

Transketolase of pork liver has been purified 90-fold and separated from ribulose 5-phosphate 3-epimerase. The transketolase is most stable between pH 7.5 and 8.5 and below 40 °C. The pH range for optimum activity is between 7.6 and 8.1. Activation by magnesium ions or thiamine pyrophosphate could not be demonstrated, but thiamine pyrophosphate increased the stability of the enzyme. Sulphydryl agents, such as p-chloromercuriphenyl sulphonic acid and N-ethylmaleimide, and heavy metal ions, such as cupric, mercuric, and zinc, at relatively high concentrations inhibited the enzyme.

The Effect of Ammonium Sulfate on the Sulphate Reducing Bacteria Bio-Derived Material

2016 ◽  
Vol 723 ◽  
pp. 529-533
Author(s):  
Guang Yuan Wang ◽  
Xing Yu Liu ◽  
Qi Yuan Gu ◽  
Ming Jiang Zhang ◽  
Jian Kang Wen
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Anaerobic Metabolism ◽  
Inorganic Nitrogen ◽  
Inorganic Nitrogen Source ◽  
Sulphate Reducing Bacteria ◽  
Autotrophic Bacteria ◽  
Lead Zinc ◽  
Reducing Bacteria

The lead-zinc tailings, a kind of hazardous wastes, containing large amount of heavy metals with high extraction toxicity, which would be released into solution with water, oxygen, microorganism and make the environment contaminated. Sulfide ion and alkaline substance were produced with the growth and anaerobic metabolism of sulphate reducing bacteria, inhibiting the oxidation and release of heavy metal ions from the lead-zinc tailings. Sulphate reducing bacteria attached on the calcined activated carbon while growing in the nutrient solution, which could be used to decline heavy metal ions as bio-derived material. Inorganic nitrogen source could be used by autotrophic bacteria effectively and was negative for the sulphate reducing bacteria to deposit toxic elements in the solution.

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