Improving the catalytic activity of hyperthermophilic Pyrococcus prolidases for detoxification of organophosphorus nerve agents over a broad range of temperatures

2010 ◽  
Vol 87 (5) ◽  
pp. 1715-1726 ◽  
Author(s):  
Casey M. Theriot ◽  
Xuelian Du ◽  
Sherry R. Tove ◽  
Amy M. Grunden
Keyword(s):  
Catalytic Activity ◽  
Nerve Agents ◽  
Organophosphorus Nerve Agents

scholarly journals Improving the Catalytic Activity of HyperthermophilicPyrococcus horikoshiiProlidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures

Archaea ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Casey M. Theriot ◽  
Rebecca L. Semcer ◽  
Saumil S. Shah ◽  
Amy M. Grunden
Keyword(s):  
Catalytic Activity ◽  
Nerve Agents ◽  
Nerve Agent ◽  
Structural Factors ◽  
Wild Type ◽  
Protein Thermostability ◽  
Pyrococcus Horikoshii ◽  
Organophosphorus Nerve Agents ◽  
Op Nerve Agents ◽  
Ph Range

Prolidases hydrolyze Xaa-Pro dipeptides and can also cleave the P-F and P-O bonds found in organophosphorus (OP) compounds, including the nerve agents soman and sarin.Ph1prol (PH0974) has previously been isolated and characterized fromPyrococcus horikoshiiand was shown to have higher catalytic activity over a broader pH range, higher affinity for metal, and increased thermostability compared toP. furiosusprolidase,Pfprol (PF1343). To obtain a better enzyme for OP nerve agent decontamination and to investigate the structural factors that may influence protein thermostability and thermoactivity, randomly mutatedPh1prol enzymes were prepared. FourPh1prol mutants (A195T/G306S-, Y301C/K342N-, E127G/E252D-, and E36V-Ph1prol) were isolated which had greater thermostability and improved activity over a broader range of temperatures against Xaa-Pro dipeptides and OP nerve agents compared to wild typePyrococcusprolidases.

Catalytic Detoxification of Organophosphorus Nerve Agents by Butyrylcholinesterase-Polymer-Oxime Bioscavengers

2020 ◽  
Vol 21 (9) ◽  
pp. 3867-3877
Author(s):  
Libin Zhang ◽  
Hironobu Murata ◽  
Gabriel Amitai ◽  
Paige N. Smith ◽  
Krzysztof Matyjaszewski ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Organophosphorus Nerve Agents

ChemInform Abstract: Design, Synthesis and Evaluation of New α-Nucleophiles for the Hydrolysis of Organophosphorus Nerve Agents: Application to the Reactivation of Phosphorylated Acetylcholinesterase.

ChemInform ◽  
2011 ◽  
Vol 42 (52) ◽  
pp. no-no
Author(s):  
Geraldine Saint-Andre ◽  
Maria Kliachyna ◽  
Sanjeevarao Kodepelly ◽  
Ludivine Louise-Leriche ◽  
Emilie Gillon ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Design Synthesis ◽  
Organophosphorus Nerve Agents ◽  
Hydrolysis Of

Phosphylated tyrosine in albumin as a biomarker of exposure to organophosphorus nerve agents

2007 ◽  
Vol 81 (9) ◽  
pp. 627-639 ◽  
Author(s):  
Nichola H. Williams ◽  
John M. Harrison ◽  
Robert W. Read ◽  
Robin M. Black
Keyword(s):  
Nerve Agents ◽  
Biomarker Of Exposure ◽  
Organophosphorus Nerve Agents ◽  
Phosphylated Tyrosine

Development of a Guinea Pig Model for Low-Dose, Long-Term Exposure to Organophosphorus Nerve Agents

2004 ◽  
Vol 14 (3) ◽  
pp. 183-194 ◽  
Author(s):  
Chessley R. Atchison ◽  
Robert E. Sheridan ◽  
Steven M. Duniho ◽  
Tsung-Ming Shih
Keyword(s):  
Guinea Pig ◽  
Low Dose ◽  
Nerve Agents ◽  
Pig Model ◽  
Organophosphorus Nerve Agents ◽  
Guinea Pig Model

Zirconium-Based Metal–Organic Frameworks for the Catalytic Hydrolysis of Organophosphorus Nerve Agents

2020 ◽  
Vol 12 (13) ◽  
pp. 14702-14720 ◽  
Author(s):  
Kent O. Kirlikovali ◽  
Zhijie Chen ◽  
Timur Islamoglu ◽  
Joseph T. Hupp ◽  
Omar K. Farha
Keyword(s):  
Metal Organic Frameworks ◽  
Nerve Agents ◽  
Catalytic Hydrolysis ◽  
Organophosphorus Nerve Agents ◽  
Metal Organic ◽  
Hydrolysis Of

Analogues with Fluorescent Leaving Groups for Screening and Selection of Enzymes That Efficiently Hydrolyze Organophosphorus Nerve Agents

2006 ◽  
Vol 49 (1) ◽  
pp. 246-255 ◽  
Author(s):  
Luis Briseño-Roa ◽  
Jim Hill ◽  
Stuart Notman ◽  
David Sellers ◽  
Andy P. Smith ◽  
...  
Keyword(s):  
Nerve Agents ◽  
Organophosphorus Nerve Agents ◽  
Leaving Groups ◽  
Selection Of
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