Temporal and spatial distribution of Bacillus and Clostridium histolyticum in swine manure composting by fluorescent in situ hybridization (FISH)

Background: Long-range transport of dust aerosol has intense impacts on theatmospheric environment over wide areas. Methods: The annual and seasonal changes inmeteorological parameters associated with the occurrence of dust storms were studied. Thefeatures of an intense dust storm and its transport characteristics were studied during June 7thto June 9th 2010 in Ahvaz city. Temporal and spatial distribution of Middle Eastern dust stormevent was analyzed by models of HYSPLIT and WRF/Chem, and in- situ observations. Results:A disagreement between the occurrences of dust storms, temperature, relative humidity andrainfall, show the major source of dust storms over Ahvaz city are neighboring countries. UsingHYSPLIT results, the dust particles are mainly transported from north western region of Iraqand eastern Syria to downward areas including Ahvaz city. The arrived Dust aerosols mixedwith local anthropogenic emissions, led to the highest PM10 concentration of 4200 ppm. Themodel results were found to well reproduce temporal and spatial distribution of mineral dustconcentrations according to in-situ measurements. Conclusion: The performance of WRF/Chemwas acceptable for simulation of temporal and spatial distributions of dust storm events.Therefore, it can be taken as a reference in daily air quality forecasting.

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Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes

Applied and Environmental Microbiology ◽

10.1128/aem.64.9.3336-3345.1998 ◽

1998 ◽

Vol 64 (9) ◽

pp. 3336-3345 ◽

Cited By ~ 736

Author(s):

Alison H. Franks ◽

Hermie J. M. Harmsen ◽

Gerwin C. Raangs ◽

Gijsbert J. Jansen ◽

Frits Schut ◽

...

Keyword(s):

In Situ Hybridization ◽

16S Rrna ◽

Fluorescent In Situ Hybridization ◽

Blot Hybridization ◽

Dry Weight ◽

Oligonucleotide Probes ◽

Fecal Flora ◽

Dot Blot ◽

Clostridium Histolyticum

ABSTRACT Six 16S rRNA-targeted oligonucleotide probes were designed, validated, and used to quantify predominant groups of anaerobic bacteria in human fecal samples. A set of two probes was specific for species of the Bacteroides fragilis group and the speciesBacteroides distasonis. Two others were designed to detect species of the Clostridium histolyticum and theClostridium lituseburense groups. Another probe was designed for the genera Streptococcus andLactococcus, and the final probe was designed for the species of the Clostridium coccoides-Eubacterium rectalegroup. The temperature of dissociation of each of the probes was determined. The specificities of the probes for a collection of target and reference organisms were tested by dot blot hybridization and fluorescent in situ hybridization (FISH). The new probes were used in initial FISH experiments to enumerate human fecal bacteria. The combination of the two Bacteroides-specific probes detected a mean of 5.4 × 1010 cells per g (dry weight) of feces; the Clostridium coccoides-Eubacterium rectalegroup-specific probe detected a mean of 7.2 × 1010cells per g (dry weight) of feces. The Clostridium histolyticum, Clostridium lituseburense, andStreptococcus-Lactococcus group-specific probes detected only numbers of cells ranging from 1 × 107 to 7 × 108 per g (dry weight) of feces. Three of the newly designed probes and three additional probes were used in further FISH experiments to study the fecal flora composition of nine volunteers over a period of 8 months. The combination of probes was able to detect at least two-thirds of the fecal flora. The normal biological variations within the fecal populations of the volunteers were determined and indicated that these variations should be considered when evaluating the effects of agents modulating the flora.

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Temporal and spatial distribution of phytoplankton as detected by in vivo et in situ fluorometry

SIL Proceedings 1922-2010 ◽

10.1080/03680770.1980.11897083 ◽

1981 ◽

Vol 21 (2) ◽

pp. 756-762

Author(s):

E. A. Nusch ◽

P. Koppe

Keyword(s):

Spatial Distribution ◽

Temporal And Spatial Distribution ◽

Temporal And Spatial

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Ultrastructural analysis of the spatial distribution of MRNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123167 ◽

1992 ◽

Vol 50 (1) ◽

pp. 552-553

Author(s):

Gary Bassell ◽

Robert H. Singer

Keyword(s):

Electron Microscopy ◽

Spatial Distribution ◽

In Situ Hybridization ◽

High Resolution ◽

Nucleic Acids ◽

Colloidal Gold ◽

Dna Probe ◽

Nucleotide Analogs ◽

Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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