Improvement of specific growth rate of Pichia pastoris for effective porcine interferon-α production with an on-line model-based glycerol feeding strategy

2011 ◽  
Vol 93 (4) ◽  
pp. 1437-1445 ◽  
Author(s):  
Min-Jie Gao ◽  
Zhi-Yong Zheng ◽  
Jian-Rong Wu ◽  
Shi-Juan Dong ◽  
Zhen Li ◽  
...  
Keyword(s):  
Growth Rate ◽  
Pichia Pastoris ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Feeding Strategy ◽  
Interferon Α ◽  
Line Model ◽  
Model Based ◽  
On Line ◽  
Glycerol Feeding

Effect of density on growth and survival of ornate rock lobster, Panulirus ornatus (Fabricius, 1798), in a flow-through raceway system

2001 ◽  
Vol 52 (8) ◽  
pp. 1425 ◽  
Author(s):  
C. M. Jones ◽  
L. Linton ◽  
D. Horton ◽  
W. Bowman
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Feeding Strategy ◽  
Experimental Unit ◽  
Rock Lobster ◽  
Growth And Survival ◽  
Mean Size ◽  
Monthly Sample ◽  
Flow Through

Juvenile ornate rock lobsters (Panulirus ornatus) (3.240.09 g; 13.80.13 mm CL) captured from the wild were stocked at three densities (14, 29, and 43 m–2) within each of four 4000-L fibreglass raceway tanks with flow-through seawater supply. Lobsters were provided with shelters consisting of opaque polyethylene platforms, 600 mm × 600 mm, supported on six 100-mm legs and were fed continually through the night with a commercial penaeid prawn (P. japonicus) pellet supplemented with prawn flesh once per day. Growth and survival were monitored by means of a monthly sample of 20 lobsters from each experimental unit. After 272 days, density treatments did not differ significantly in survival, which averaged 52.5% (2.8). Lobster size was also unaffected by density, and mean size for all lobsters was 225.34.68 g (61.84.7 mm CL) at harvest. Mortality was consistent through time and was almost entirely attributable to cannibalism of postmoult individuals. The cannibalism may have been due to inappropriate shelter and feeding strategy. Despite higher mortality than anticipated, growth was rapid, representing a specific growth rate of 1.56% day–1, sufficient to permit growth from 3 g to 1 kg within 18 months. The experiment confirmed the excellent potential of P. ornatus for commercial aquaculture.

An algorithmic approach to constructing the on-line estimation system for the specific growth rate

1989 ◽  
Vol 33 (3) ◽  
pp. 354-364 ◽  
Author(s):  
Hiroshi Shimizu ◽  
Takeichiro Takamatsu ◽  
Suteaki Shioya ◽  
Ken-Ichi Suga
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Algorithmic Approach ◽  
On Line ◽  
Estimation System

Automated fed-batch culture of Kluyveromyces fragilis based on a novel method for on-line estimation of cell specific growth rate

2001 ◽  
Vol 9 (3) ◽  
pp. 221-231 ◽  
Author(s):  
Zairossani M. Nor ◽  
Melih I. Tamer ◽  
Jeno M. Scharer ◽  
Murray Moo-Young ◽  
Eric J. Jervis
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Batch Culture ◽  
Specific Growth ◽  
Fed Batch ◽  
Kluyveromyces Fragilis ◽  
On Line ◽  
Novel Method ◽  
Fed Batch Culture

scholarly journals Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Javier Garrigós-Martínez ◽  
Miguel Angel Nieto-Taype ◽  
Arnau Gasset-Franch ◽  
José Luis Montesinos-Seguí ◽  
Xavier Garcia-Ortega ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Rate ◽  
Pichia Pastoris ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Gene Dosage ◽  
Fed Batch ◽  
Komagataella Phaffii ◽  
Production Kinetics ◽  
Kinetics Of

Abstract Background The PAOX1-based expression system is the most widely used for producing recombinant proteins in the methylotrophic yeast Pichia pastoris (Komagataella phaffii). Despite relevant recent advances in regulation of the methanol utilization (MUT) pathway have been made, the role of specific growth rate (µ) in AOX1 regulation remains unknown, and therefore, its impact on protein production kinetics is still unclear. Results The influence of heterologous gene dosage, and both, operational mode and strategy, on culture physiological state was studied by cultivating the two PAOX1-driven Candida rugosa lipase 1 (Crl1) producer clones. Specifically, a clone integrating a single expression cassette of CRL1 was compared with one containing three cassettes over broad dilution rate and µ ranges in both chemostat and fed-batch cultivations. Chemostat cultivations allowed to establish the impact of µ on the MUT-related MIT1 pool which leads to a bell-shaped relationship between µ and PAOX1-driven gene expression, influencing directly Crl1 production kinetics. Also, chemostat and fed-batch cultivations exposed the favorable effects of increasing the CRL1 gene dosage (up to 2.4 fold in qp) on Crl1 production with no significant detrimental effects on physiological capabilities. Conclusions PAOX1-driven gene expression and Crl1 production kinetics in P. pastoris were successfully correlated with µ. In fact, µ governs MUT-related MIT1 amount that triggers PAOX1-driven gene expression—heterologous genes included—, thus directly influencing the production kinetics of recombinant protein.

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