Over-expression of native Saccharomyces cerevisiae exocytic SNARE genes increased heterologous cellulase secretion

2014 ◽  
Author(s):  
John Henry D. Van Zyl ◽  
Riaan Den Haan ◽  
Willem H. Van Zyl
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Over Expression ◽  
Cellulase Secretion

scholarly journals Expression of human glutathione S-transferases in Saccharomyces cerevisiae confers resistance to the anticancer drugs adriamycin and chlorambucil

1990 ◽  
Vol 268 (2) ◽  
pp. 309-315 ◽  
Author(s):  
S M Black ◽  
J D Beggs ◽  
J D Hayes ◽  
A Bartoszek ◽  
M Muramatsu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Anticancer Drugs ◽  
Direct Evidence ◽  
Evolutionary Process ◽  
Resistance Mechanisms ◽  
Detoxification Enzymes ◽  
Yeast Saccharomyces Cerevisiae ◽  
Cytotoxic Effects ◽  
Over Expression ◽  
Glutathione S Transferases

Adaptation and resistance to chemicals in the environment is a critical part of the evolutionary process. As a result, a wide variety of defence systems that protect cells against chemical insult have evolved. Such chemical resistance mechanisms appear to play a central role in determining the sensitivity of human tumours to treatment with chemotherapeutic drugs. The glutathione S-transferases (GST) are important detoxification enzymes whose over-expression has been associated with drug-resistance. In order to evaluate this possibility we have expressed the human Alpha-class and Pi-class GST cDNAs that encode GST B1B1 and GST pi in the yeast Saccharomyces cerevisiae. The expression of GST B1B1 or GST pi resulted in a marked reduction in the cytotoxic effects of chlorambucil, a bifunctional alkylating agent, and an anthracycline, adriamycin. These data provide direct evidence that the over-expression of GST in cells can confer resistance to anticancer drugs.

YAP1 over-expression in Saccharomyces cerevisiae enhances glutathione accumulation at its biosynthesis and substrate availability levels

2011 ◽  
Vol 7 (4) ◽  
pp. 566-568 ◽  
Author(s):  
Kerti Orumets ◽  
Kaspar Kevvai ◽  
Ildar Nisamedtinov ◽  
Tiina Tamm ◽  
Toomas Paalme
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Substrate Availability ◽  
Over Expression
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