Durian Locule (Endocarp) Water Immersion Drinking Effect to Reduce Heaty Sensation after Flesh Consumption: A Preliminary Study

Durian is said to have a “heaty” effect on the people who have eaten it that can raise their body temperature and blood pressure. The locule water immersion is the water that is drunk using the durian’s inner skin (endocarp) that contains the durian flesh and it is said (mainly via local hearsay) that it can lower the body temperature right after consuming the flesh. The aim of this research is to investigate a myth about the effect of D24 durian locule water immersion that can possibly reduce body temperature after eating durian via oral temperature assessment. In order to explore the reliability of this myth, an experimental research was carried out with five different respondents to undergo with 3 different set of condition which are: a) consumed the same amount of durian, but they did not have to drink the immersed-locule water; b) consumed the durian and they had to drink the immersed-locule water and c) consumed the durian and they had to drink a cup of water. The changes in their body temperature (oral reading) were recorded and analysed for significant changes (n = 3). Overall, the immersed-locule water exhibited a mild affect in the changes of body temperature (p<0.05) on a short period of time (<30 mins after consumption). For that reason, the availability of pectin in the locule water-immersion might have help facilitates the natural homeostasis mechanism faster as to suppress of any sudden body heating after eating durian.

MicroRNA-705 regulates the differentiation of mouse mandible bone marrow mesenchymal stem cells

The craniofacial skeleton is the foundation of most stomatological treatments, including prosthodontics and maxillofacial surgery. Although histologically similar to the appendicular skeleton, the craniofacial skeleton manifests many unique properties in response to external stimuli and signals. However, the mandibular or maxillary bone marrow mesenchyme, which is the intrinsic foundation of the functions of craniofacial skeleton, has not been well studied, and its homeostasis mechanism remains elusive. Osteoporosis is a systemic disease that affects all skeletons and is characterized by bone mass loss. Osteoporotic bone marrow mesenchymal stem cells (BMMSCs) exhibit disturbed homeostasis and distorted lineage commitment. Many reports have shown that microRNAs (miRNAs) play important roles in regulating MSCs homeostasis. Here, to obtain a better understanding of mandibular bone marrow MSCs homeostasis, we isolated and cultured mandible marrow MSCs from mouse mandibles. Using miR-705 mimics and an inhibitor, we demonstrated that miR-705 played a vital role in shifting the mandibular MSCs lineage commitment in vitro. Utilizing an osteoporosis mouse model, we demonstrated that MSCs from ovariectomized (OVX) mouse mandibular bone marrow exhibited impaired osteogenic and excessive adipogenic differentiation. miR-705 was found overexpressed in OVX mandibular MSCs. The knock down of miR-705 in vitro partially attenuated the differentiation disorder of the OVX mandibular MSCs by upregulating the expression of osteogenic marker genes but suppressing adipogenic genes. Taken together, our findings provide a better understanding of the homeostasis mechanism of mandibular BMMSCs and a novel potential therapeutic target for treating mandibular osteoporosis.