scholarly journals Current advances in molecular methods for detection of nitrite-dependent anaerobic methane oxidizing bacteria in natural environments

2016 ◽  
Vol 100 (23) ◽  
pp. 9845-9860 ◽  
Author(s):  
Jing Chen ◽  
Richard Dick ◽  
Jih-Gaw Lin ◽  
Ji-Dong Gu
Author(s):  
L. P. Hardie ◽  
D. L. Balkwill ◽  
S. E. Stevens

Agmenellum quadruplicatum is a unicellular, non-nitrogen-fixing, marine cyanobacterium (blue-green alga). The ultrastructure of this organism, when grown in the laboratory with all necessary nutrients, has been characterized thoroughly. In contrast, little is known of its ultrastructure in the specific nutrient-limiting conditions typical of its natural habitat. Iron is one of the nutrients likely to limit this organism in such natural environments. It is also of great importance metabolically, being required for both photosynthesis and assimilation of nitrate. The purpose of this study was to assess the effects (if any) of iron limitation on the ultrastructure of A. quadruplicatum. It was part of a broader endeavor to elucidate the ultrastructure of cyanobacteria in natural systemsActively growing cells were placed in a growth medium containing 1% of its usual iron. The cultures were then sampled periodically for 10 days and prepared for thin sectioning TEM to assess the effects of iron limitation.


Author(s):  
T.A. Fassel ◽  
M.J. Schaller ◽  
M.E. Lidstrom ◽  
C.C. Remsen

Methylotrophic bacteria play an Important role in the environment in the oxidation of methane and methanol. Extensive intracytoplasmic membranes (ICM) have been associated with the oxidation processes in methylotrophs and chemolithotrophic bacteria. Classification on the basis of ICM arrangement distinguishes 2 types of methylotrophs. Bundles or vesicular stacks of ICM located away from the cytoplasmic membrane and extending into the cytoplasm are present in Type I methylotrophs. In Type II methylotrophs, the ICM form pairs of peripheral membranes located parallel to the cytoplasmic membrane. Complex cell wall structures of tightly packed cup-shaped subunits have been described in strains of marine and freshwater phototrophic sulfur bacteria and several strains of methane oxidizing bacteria. We examined the ultrastructure of the methylotrophs with particular view of the ICM and surface structural features, between representatives of the Type I Methylomonas albus (BG8), and Type II Methylosinus trichosporium (OB-36).


2013 ◽  
Vol 18 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Emmanuel Kuntsche ◽  
Florian Labhart

Ecological Momentary Assessment (EMA) is a way of collecting data in people’s natural environments in real time and has become very popular in social and health sciences. The emergence of personal digital assistants has led to more complex and sophisticated EMA protocols but has also highlighted some important drawbacks. Modern cell phones combine the functionalities of advanced communication systems with those of a handheld computer and offer various additional features to capture and record sound, pictures, locations, and movements. Moreover, most people own a cell phone, are familiar with the different functions, and always carry it with them. This paper describes ways in which cell phones have been used for data collection purposes in the field of social sciences. This includes automated data capture techniques, for example, geolocation for the study of mobility patterns and the use of external sensors for remote health-monitoring research. The paper also describes cell phones as efficient and user-friendly tools for prompt manual data collection, that is, by asking participants to produce or to provide data. This can either be done by means of dedicated applications or by simply using the web browser. We conclude that cell phones offer a variety of advantages and have a great deal of potential for innovative research designs, suggesting they will be among the standard data collection devices for EMA in the coming years.


2008 ◽  
Vol 37 (3) ◽  
Author(s):  
Jacek Urbański ◽  
Agata Ślimak

Assessing flood risk and detecting changes of salt water inflow in a coastal micro-tidal brackish marsh using GISIn order to assess changes in salt water inflow and potential flood risks due to sea level rise in a micro-tidal Beka brackish marsh on the Polish Baltic Coast GIS was used. Such wetlands are important elements of coastal zone natural environments. Creating a geodatabase within a GIS system makes it possible to carry out broad analyses of complex systems, such as coastal wetlands. The results indicate that a 40 cm sea-level rise would considerably increase the frequency of flooding in the investigated area, in part because of the small range of the annual sea level oscillations there. A map of the index of changes in saltwater inflow, created with the help of cost-weighted distance (functions), shows that changes which have occurred along the shore, consisting of filling in the drainage channel outlets, have likely had a significant impact on the vegetation of the area.


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